Abstract:
:Objective: The aim of this study was to investigate the molecular mechanism of inflammasome activation in response to Streptococcus suis serotype 2 (SS2) infection and its contribution to the development of streptococcal toxic shock-like syndrome (STSS). Methods: To verify the role of suilysin (SLY) in STSS, we infected bone-marrow-derived macrophages (BMDMs) in vitro and C57BL/6J mice intraperitoneally (IP) with the SS2 wild-type (WT) strain or isogenic sly mutant (∆SLY) to measure the interleukin (IL)-1β release and survival rate. To determine the role of inflammasome activation and pyroptosis in STSS, we infected BMDMs from WT and various deficient mice, including Nlrp3-deficient (Nlrp3-/-), Nlrc4-deficient (Nlrc4-/-), Asc-deficient (Asc-/-), Aim2-deficient (Aim2-/-), Caspase-1/11-deficient (Caspase-1/11-/-), and Gsdmd-deficient (Gsdmd-/-) ex vivo, and IP injected WT, Nlrp3-/-, Caspase-1/11-/-, and Gsdmd-/- mice with SS2, to compare the IL-1β releases and survival rate in vivo. Results: The SS2-induced IL-1β production in mouse macrophages is mediated by SLY ex vivo. The survival rate of WT mice infected with SS2 was significantly lower than that of mice infected with the ∆SLY strain in vivo. Furthermore, SS2-triggered IL-1β releases, and the cytotoxicity in the BMDMs required the activation of the NOD-Like Receptors Family Pyrin Domain Containing 3 (Nlrp3), Caspase-1/11, and gasdermin D (Gsdmd) inflammasomes, but not the Nlrc4 and Aim2 inflammasomes ex vivo. The IL-1β production and survival rate of WT mice infected with SS2 were significantly lower than those of the Nlrp3-/-, Caspase-1/11-/-, and Gsdmd-/- mice in vivo. Finally, the inhibitor of the Nlrp3 inflammasome could reduce the IL-1β release and cytotoxicity of SS2-infected macrophages ex vivo and protect SS2-infected mice from death in vivo. Conclusion: Nlrp3 inflammasome activation triggered by SLY in macrophages played an important role in the pathogenesis of STSS.
journal_name
Front Microbioljournal_title
Frontiers in microbiologyauthors
Song L,Li X,Xiao Y,Huang Y,Jiang Y,Meng G,Ren Zdoi
10.3389/fmicb.2020.01788subject
Has Abstractpub_date
2020-08-14 00:00:00pages
1788issn
1664-302Xjournal_volume
11pub_type
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