Abstract:
:The antifungal ionophore nystatin dissipates the Na(+) and K(+) gradients across the cell membrane, leading to cellular gain of Na(+) and cellular loss of K(+) . The increase of cellular Na(+) concentration may result in Ca(2+) accumulation in exchange for Na(+) . Increase of cytosolic Ca(2+) activity ([Ca(2+) ]i ) and loss of cellular K(+) foster apoptosis-like suicidal erythrocyte death or eryptosis, which is characterised by cell shrinkage and cell membrane scrambling leading to phosphatidylserine exposure at the erythrocyte surface. The present study explored whether nystatin stimulates eryptosis. Cell volume was estimated from forward scatter (FSC), phosphatidylserine exposure from annexin V binding and [Ca(2+) ]i from Fluo3-fluorescence in flow cytometry. A 48-hr exposure to nystatin (15 μg/ml) was followed by a significant increase of [Ca(2+) ]i , a significant increase of annexin V binding and a significant decrease of FSC. The annexin V binding after nystatin treatment was significantly blunted in the nominal absence of extracellular Ca(2+) . Partial replacement of extracellular Na(+) with extracellular K(+) blunted the nystatin-induced erythrocyte shrinkage but increased [Ca(2+) ]i and annexin V binding. Nystatin triggers cell membrane scrambling, an effect at least partially due to entry of extracellular Ca(2+) .
journal_name
Basic Clin Pharmacol Toxicoljournal_title
Basic & clinical pharmacology & toxicologyauthors
Malik A,Bissinger R,Jilani K,Lang Fdoi
10.1111/bcpt.12279subject
Has Abstractpub_date
2015-01-01 00:00:00pages
47-52issue
1eissn
1742-7835issn
1742-7843journal_volume
116pub_type
杂志文章abstract::Vitamin K antagonists (VKA) are highly effective anticoagulants but their use is hampered by multiple interactions with food and medicine and a narrow therapeutic range. The large variation in dose requirements has led to the development of several dosing algorithms based on pharmacogenetic and clinical variables. In ...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/j.1742-7843.2011.00789.x
更新日期:2012-02-01 00:00:00
abstract::The present study investigated the improving effect of cucurbitacin B on liver fibrosis induced by concanavalin A in mice and explored its possible mechanism. AST, ALT and TB were detected by kits. ELISA was performed to detect the levels of IL 5, IL 6, IL 13 and TNF-α in serum. Haematoxylin-eosin (HE) staining and Ma...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/bcpt.13446
更新日期:2020-11-01 00:00:00
abstract::Effect of carbon tetrachloride (CCl(4)) pretreatment on the biotransformation and elimination of acetaminophen were examined in male mice. A 24 hr initial dose of CCl(4) (0.05 ml/kg, intraperitioneally) reduced the induction of hepatotoxicity resulting from acetaminophen treatment (350 mg/kg, intraperitoneally) as det...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/j.1742-7843.2006.pto_308.x
更新日期:2006-02-01 00:00:00
abstract::This work demonstrates how full modelling power in statistically mixed models can be used to study generalizability (reliability) coefficients of advanced data from human experimental pain studies utilizing placebo data from drug screening trials. This can be used to help optimizing outcome parameters from existing da...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1742-7843.2009.00408.x
更新日期:2009-08-01 00:00:00
abstract::The endothelium plays crucial roles in modulating vascular tone by synthesizing and releasing endothelium-derived relaxing factors (EDRFs), including vasodilator prostaglandins, nitric oxide (NO) and endothelium-dependent hyperpolarization (EDH) factors. Thus, endothelial dysfunction is the hallmark of atherosclerotic...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章,评审
doi:10.1111/bcpt.13377
更新日期:2020-08-01 00:00:00
abstract::Tamoxifen has been suggested to produce beneficial cardiovascular effects, although the mechanisms for these effects are not fully known. Moreover, although tamoxifen metabolites may exhibit 30-100 times higher potency than the parent drug, no previous study has compared the effects produced by tamoxifen and its metab...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/j.1742-7843.2009.00377.x
更新日期:2009-05-01 00:00:00
abstract::Genetic polymorphisms influence the metabolism of ethanol and methanol, but the potential effects of genetic predisposition on the clinical course, outcome and short-term health sequelae of acute methanol poisoning are unknown. To evaluate the role of the MEOS system in methanol poisoning, we analysed the effect of th...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/bcpt.12310
更新日期:2015-02-01 00:00:00
abstract::Newly developed acetylcholinesterase reactivators K117 [1,5-bis(4-hydroxyiminomethylpyridinium)-3-oxapentane dichloride] and K127 [(1-(4-hydroxyiminomethylpyridinium)-5-(4-carbamoylpyridinium)-3-oxapentane dibromide)] were tested for their potency to reactivate tabun-inhibited human brain cholinesterases. Pralidoxime ...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/j.1742-7843.2009.00421.x
更新日期:2009-09-01 00:00:00
abstract::In this study, we tested the hypothesis that fasudil, a Rho kinase inhibitor, would protect against contrast-induced acute kidney injury (CI-AKI) in a mouse model and attempted to elucidate the mechanism involved. Mice subjected to unilateral ligation of the left anterior renal pedicle were divided into four groups: (...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/bcpt.12895
更新日期:2018-02-01 00:00:00
abstract::The Danish Poison Information Centre (DPIC) regularly receives inquiries about nursing home residents who have been exposed to a medication error. The aim of this prospective cohort study was to describe and discuss the types and consequences of these errors. Data were collected from 1 March 2018 to 31 March 2019. Reg...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/bcpt.13529
更新日期:2020-11-04 00:00:00
abstract::Cigarette smoking is the principal cause of chronic obstructive pulmonary disease (COPD), especially emphysema, which is characterized by alveolar wall destruction and airspace enlargement. Apoptosis of lung structural cells is involved in the pathogenesis of COPD. Xanthine derivatives (aminophylline or theophylline) ...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/j.1742-7843.2011.00673.x
更新日期:2011-07-01 00:00:00
abstract::Simvastatin (SV), a HMG-CoA reductase inhibitor, is widely used for the treatment of hyperlipidaemia. The objectives of the present study were to develop a population pharmacokinetic-pharmacodynamic (PK-PD) model for simvastatin and to evaluate its usefulness in predicting the dose-response relationship of simvastatin...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/j.1742-7843.2011.00700.x
更新日期:2011-09-01 00:00:00
abstract::Quercetin is a plant flavonoid with several biological activities. This study aimed to describe quercetin effects on contractile and electrophysiological properties of the cardiac muscle as well as on calcium handling. Quercetin elicited positive inotropism that was significantly reduced by propranolol indicating an i...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/bcpt.12743
更新日期:2017-06-01 00:00:00
abstract::Upper gastrointestinal bleeding is a feared complication of using non-steroidal anti-inflammatory drugs (NSAIDs) or aspirin. Studies predicting the incidence rate for individuals with a given set of characteristics are lacking. The aim of this study was to develop a risk model to predict the incidence rate of upper ga...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/bcpt.13370
更新日期:2020-05-01 00:00:00
abstract::Seijo-bofu-to, a traditional medicine used to treat acne in Asian countries, contains twelve herbal components, including Angelica dahurica root, a source of furanocoumarin derivatives. In this study, we investigated potential herb-drug interactions of seijo-bofu-to in healthy male volunteers. Thirty-two young, health...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/bcpt.12224
更新日期:2014-10-01 00:00:00
abstract::The use of herbal or natural medicines for the treatment of various disorders has a long and extensive history. Many of these herbal medicines are finding their way onto the world market as alternatives to prescribed drugs currently available to treat various disorders/ailments. In particular, hyperlipidaemia is a maj...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章,评审
doi:10.1111/j.1742-7843.2005.pto960102.x
更新日期:2005-01-01 00:00:00
abstract::Trimidox (3,4,5-trihydroxybenzamidoxime) has been shown to reduce the activity of ribonucleotide reductase accompanied by growth inhibition and the differentiation of mammalian cells. Here we examine the induction of apoptosis by trimidox in several human leukaemia cell lines, focusing on the release of cytochrome c a...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/j.1742-7843.2006.pto_188.x
更新日期:2006-01-01 00:00:00
abstract::N-methyl-D-aspartate (NMDA) receptors are ionotropic glutamate receptors widely distributed in the central nervous system, and have been extensively investigated for their roles in embryonic development, synaptic plasticity and neuroexcitoxicity. Their functions in the peripheral nervous system and non-neural tissues ...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/j.1742-7843.2010.00572.x
更新日期:2010-09-01 00:00:00
abstract::Melatonin possesses chronobiotic properties, which affects sleep/wake rhythms. We investigated a 7-day administration of melatonin (0.25, 2.5 and 5 mg/kg body weight) on the activity/rest rhythms of a diurnal animal (the ringdove, Streptopelia risoria), aged 2-3 (young) and 10-12 (old) years, and its possible relation...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/j.1742-7843.2006.00032.x
更新日期:2007-04-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is the third most common cause of cancer-related death. In patients for whom HCC could not be detected early, current treatments show poor tolerance and low efficacy. So, alternative therapies with good efficacy are urgently needed. The aim of this research was to evaluate the selective ...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/bcpt.12572
更新日期:2016-09-01 00:00:00
abstract::In view of paediatric drug development, regulatory authorities often request safety studies in juvenile animals, including minipigs. Unfortunately, knowledge on the ontogeny of the biotransformation processes in animal models remains scarce and impedes a correct interpretation of the toxicity findings. CYP3A4 is one o...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/bcpt.12410
更新日期:2015-11-01 00:00:00
abstract::Retinal arachidonic acid (ARA) levels in form-deprived eyes decline in guinea pigs. As prostaglandin F2α (PGF2α) is an ARA metabolite and endogenous agonist of prostaglandin F receptor (FP), we have been suggested that down-regulation of PGF2α-FP receptor signalling pathway contributes to myopia onset. To test this hy...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/bcpt.13017
更新日期:2018-09-01 00:00:00
abstract::δ-Containing GABAA receptors are located extrasynaptically and mediate tonic inhibition. Their involvement in brain physiology positions them as interesting drug targets. There is thus a continued interest in establishing reliable recombinant expression systems for δ-containing GABAA receptors. Inconveniently, the rec...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/bcpt.12778
更新日期:2017-08-01 00:00:00
abstract::Young mice (2 weeks old) were given topiramate daily for 1 month, and sudomotor function was evaluated utilizing impression mould techniques to determine the number of sweat glands reactive to heat exposure and sweat output per gland on the plantar surface of mice hind-paws. Immunohistochemical quantitation of protein...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/j.1742-7843.2007.00062.x
更新日期:2007-06-01 00:00:00
abstract::Extrasynaptic δ subunit-containing γ-aminobutyric acid type A receptors (δ-GABAA Rs) are emerging as targets for a number of neuropsychopharmacological drugs, including the direct GABA site agonist gaboxadol and neuroactive steroids. Among other regions, these δ-GABAA Rs are functionally expressed in the ventral tegme...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/bcpt.13043
更新日期:2018-11-01 00:00:00
abstract::The potent neurotoxin saxitoxin (STX) belongs to a group of structurally related analogues produced by both marine and freshwater phytoplankton. The toxins act by blocking voltage-gated sodium channels stopping the inflow of sodium ions and the generation of action potentials. Exposure from marine sources occurs as a ...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/bcpt.12701
更新日期:2017-04-01 00:00:00
abstract::Snake venom components, acting in concert in the prey, cause their immobilization and initiate digestion. To achieve this, several hydrolytic enzymes of snake venom have evolved to interfere in various physiological processes, which are well defined. However, hydrolytic enzymes such as phosphatases (acid and alkaline ...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章,评审
doi:10.1111/j.1742-7843.2010.00630.x
更新日期:2011-02-01 00:00:00
abstract::Previous studies suggest that flavonol icariin protects against neuron injury after oxygen and glucose deprivation by increasing SIRT1. This study demonstrates that icariin can inhibit H(2) O(2) -induced neurotoxicity. The neuroprotection of icariin enhances the antioxidant capacity through both a direct scavenging ef...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/j.1742-7843.2010.00595.x
更新日期:2010-11-01 00:00:00
abstract::The aim was to investigate systemic exposure after administration of a novel bupivacaine lozenge in healthy individuals with normal mucosa and in head and neck cancer (HNC) patients with oral mucositis. A lozenge containing 5, 10, 25 and 50 mg bupivacaine, respectively, was administered as single dose to 10 healthy in...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/bcpt.12644
更新日期:2017-01-01 00:00:00
abstract::There is an obvious urgent need to find effective and safe therapies to prevent both recurrence and progression of bladder cancer. In the present study, we report that fisetin-induced apoptosis in human bladder cancer is mediated via modulation of two related pathways: up-regulation of p53 and down-regulation of NF-ka...
journal_title:Basic & clinical pharmacology & toxicology
pub_type: 杂志文章
doi:10.1111/j.1742-7843.2010.00613.x
更新日期:2011-02-01 00:00:00