Introduction of a mixture of β-tricalcium phosphate into a complex of bone marrow mesenchymal stem cells and type I collagen can augment the volume of alveolar bone without impairing cementum regeneration.


BACKGROUND:The purpose of this study is to evaluate whether β-tricalcium phosphate (β-TCP) could be a promising modality to help augment alveolar bone in periodontal tissue regeneration by bone marrow mesenchymal stem cells (BMMSCs). METHODS:Expanded BMMSCs and atelocollagen (Col) were mixed together (MSC/Col). A combination of β-TCP with MSC/Col was also prepared (MSC/Col/TCP). MSC/Col/TCP or MSC/Col was transplanted into experimental periodontal Class III furcation defects that had been exposed to inflammation in beagle dogs. Periodontal tissue regeneration was evaluated by histologic and morphometric analyses at 4 and 8 weeks after transplantation. RESULTS:MSC/Col and MSC/Col/TCP enhanced periodontal tissue regeneration compared to Col and TCP/Col according to hematoxylin and eosin staining. The percentage of new cementum length in the MSC/Col/TCP group was not significantly different from that in the MSC/Col group at 4 and 8 weeks. On the other hand, the percentage of new bone area in the MSC/Col/TCP group was much higher than that in the MSC/TCP group at 4 weeks. However, at 8 weeks, no significant difference in new bone area was found between the two groups. In the MSC/Col/TCP group, β-TCP was surrounded by newly formed bone. Multinucleated cells, which were positive for osteopontin and tartrate-resistant acid phosphatase, were present in the interconnected macropores of β-TCP. CONCLUSION:These findings suggest that β-TCP is applicable as a scaffold for BMMSCs transplantation and helps augment alveolar bone without impairing regeneration of cementum.


J Periodontol


Nagahara T,Yoshimatsu S,Shiba H,Kawaguchi H,Takeda K,Iwata T,Mizuno N,Fujita T,Kurihara H




Has Abstract


2015-03-01 00:00:00












  • Association between periodontitis and gestational diabetes mellitus: a case-control study.

    abstract:BACKGROUND:Some studies have reported an association between gestational diabetes mellitus (GDM) and periodontitis. The aim of the present study is to analyze this potential association and the influence of risk variables associated with GDM. METHODS:This case-control study includes 360 women, 90 with GDM and 270 cont...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Esteves Lima RP,Miranda Cota LO,Costa FO

    更新日期:2013-09-01 00:00:00

  • One stage full- versus partial-mouth disinfection in the treatment of chronic adult or generalized early-onset periodontitis. II. Long-term impact on microbial load.

    abstract:BACKGROUND:Recent studies showed the clinical benefits of a one stage full-mouth disinfection, when compared to the worldwide standard treatment strategy of consecutive root planings per quadrant without proper disinfection of the remaining intraoral niches. The purpose of this study was to investigate the microbiologi...

    journal_title:Journal of periodontology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Quirynen M,Mongardini C,Pauwels M,Bollen CM,Van Eldere J,van Steenberghe D

    更新日期:1999-06-01 00:00:00

  • Transcriptomes in healthy and diseased gingival tissues.

    abstract:BACKGROUND:Clinical and radiographic measures are gold standards for diagnosing periodontitis but offer little information regarding the pathogenesis of the disease. We hypothesized that a comparison of gene expression signatures between healthy and diseased gingival tissues would provide novel insights in the pathobio...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Demmer RT,Behle JH,Wolf DL,Handfield M,Kebschull M,Celenti R,Pavlidis P,Papapanou PN

    更新日期:2008-11-01 00:00:00

  • Hepatocellular carcinoma metastatic to the oral mucosa: report of a case with multiple gingival localizations.

    abstract:BACKGROUND:Metastases to the oral mucosa are rare, representing less than 1% of the tumors at this site. Most of these metastatic neoplasms originate in the lungs, kidneys, and liver. METHODS:The clinicopathologic features of an occult hepatocellular carcinoma, metastatic to the oral mucosa, are reported. The patient,...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Maiorano E,Piattelli A,Favia G

    更新日期:2000-04-01 00:00:00

  • Coping with stress: its influence on periodontal disease.

    abstract:BACKGROUND:Various forms of stress behavior were documented and in patients with periodontitis their relationship with periodontal disease was investigated. METHODS:Eighty-nine patients with different forms of chronic periodontitis were included in this retrospective case-control study. They were all undergoing period...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Wimmer G,Janda M,Wieselmann-Penkner K,Jakse N,Polansky R,Pertl C

    更新日期:2002-11-01 00:00:00

  • Comparisons of various anthropometric indexes with localized Stage II/III periodontitis in young adults: The CHIEF oral health study.

    abstract:BACKGROUND:A J-shaped relationship of body mass index (BMI) with severe periodontitis has been reported. However, it is unknown for other anthropometric indexes in young adults. METHODS:A cross-sectional study examined the relationships in 325 military men and women, aged 20 to 45 years in Taiwan. Anthropometric index...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Tsai KZ,Huang RY,Cheng WC,Su FY,Lin YP,Chang CY,Lin GM

    更新日期:2020-11-03 00:00:00

  • Periodontitis is associated with systemic inflammation and vascular endothelial dysfunction in patients with lacunar infarct.

    abstract:BACKGROUND:Periodontitis has been associated with lacunar infarct (LI), a type of cerebral small vessel disease. The objective of this study was to ascertain whether periodontitis is associated with increased circulating levels of systemic inflammation and endothelial dysfunction biomarkers in patients with LI. METHOD...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Leira Y,Rodríguez-Yáñez M,Arias S,López-Dequidt I,Campos F,Sobrino T,D'Aiuto F,Castillo J,Blanco J

    更新日期:2019-05-01 00:00:00

  • Use of bovine-derived hydroxyapatite in the treatment of edentulous ridge defects: a human clinical and histologic case report.

    abstract::The advent of AIDS has created patients fears regarding human tissue transfers, including bone grafts in dental surgery. In the face of these fears, alternative allograft materials are of increasing interest to clinicians. One such alternative is naturally derived xenogeneic hydroxyapatite (HA). In this study, two pat...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Callan DP,Rohrer MD

    更新日期:1993-06-01 00:00:00

  • Early soft tissue healing around one-stage dental implants: clinical and microbiologic parameters.

    abstract:BACKGROUND:Despite the potential significance of early soft tissue healing to long-term outcomes, this aspect of one-stage dental implants has not been investigated. The purpose of this prospective study was to characterize clinical and microbiologic parameters of early soft tissue healing around dental implants placed...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: DeAngelo SJ,Kumar PS,Beck FM,Tatakis DN,Leblebicioglu B

    更新日期:2007-10-01 00:00:00

  • Non-Invasive Periodontal Probing Through Fourier-Domain Optical Coherence Tomography.

    abstract:BACKGROUND:Periodontitis is a multifactorial and infectious disease that may result in significant debilitation. The aim of this study is to exploit two optical coherence tomography (OCT) systems operating in the Fourier domain at different wavelengths, 930 and 1,325 nm, for structural analysis of periodontal tissue in...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Mota CC,Fernandes LO,Cimões R,Gomes AS

    更新日期:2015-09-01 00:00:00

  • The association between Porphyromonas gingivalis-specific maternal serum IgG and low birth weight.

    abstract:BACKGROUND:In Alabama, low birth weight (LBW) infants are about 20 times more likely to die before their first birthday compared to normal birth weight infants. While the rate of LBW has been consistently higher among African Americans compared to whites, there has been a gradual increase in LBW for both African Americ...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Dasanayake AP,Boyd D,Madianos PN,Offenbacher S,Hills E

    更新日期:2001-11-01 00:00:00

  • Morbidity and economic complications following mucogingival surgery in a hemophiliac HIV-infected patient: a case report.

    abstract:BACKGROUND:This report describes the surgical treatment of a gingival recession in a hemophiliac HIV-infected patient. To our knowledge, mucogingival surgery has not been described previously in these patients. METHODS:Under the supervision of the patient's hematologist, a subepithelial connective tissue graft procedu...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Blanco-Carrion J,Liñares-Gonzalez A,Batalla-Vazquez P,Diz-Dios P

    更新日期:2004-10-01 00:00:00

  • Assessment of total antioxidant capacity and the use of vitamin C in the treatment of non-smokers with chronic periodontitis.

    abstract:BACKGROUND:Several recent studies have indicated that total antioxidant capacity (TAOC) of plasma seems to be compromised in chronic periodontitis (ChP). The aim of this study is to investigate plasma TAOC in patients with ChP and to assess the effects of vitamin C as an adjunct to non-surgical periodontal treatment. ...

    journal_title:Journal of periodontology

    pub_type: 杂志文章,随机对照试验


    authors: Abou Sulaiman AE,Shehadeh RM

    更新日期:2010-11-01 00:00:00

  • Systematic errors in estimating prevalence and severity of periodontal disease.

    abstract::Partial-mouth scores are often used in epidemiologic studies to estimate the prevalence and severity of periodontal disease. Such estimates systematically underestimate the prevalence of disease, but the bias can be in either direction for disease severity. Three large data sets containing full-mouth examinations (mes...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Kingman A,Morrison E,Löe H,Smith J

    更新日期:1988-11-01 00:00:00

  • The effect of aspirin intake on bleeding on probing in patients with gingivitis.

    abstract:BACKGROUND:Bleeding indices are used as a screen for periodontal disease activity, a measure of disease prevalence, and a measure of effectiveness in clinical trials. Bleeding on probing (BOP) is widely interpreted as a sign of disease activity whereas its absence is interpreted as both a sign and predictor of health. ...

    journal_title:Journal of periodontology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Royzman D,Recio L,Badovinac RL,Fiorellini J,Goodson M,Howell H,Karimbux N

    更新日期:2004-05-01 00:00:00

  • Pemphigus vulgaris presenting as a gingival lesion. A case report.

    abstract::A 26-year-old hispanic male presented with a nonspecific gingival lesion initially presumed to be of infectious etiology. During continued follow-up over several weeks, the lesion evolved into one typical of desquamative gingivitis. Direct immunofluorescent testing and routine histopathology resulted in a diagnosis of...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Barnett ML

    更新日期:1988-09-01 00:00:00

  • Lamina propria dendritic cells express activation markers and contact lymphocytes in chronic periodontitis.

    abstract:BACKGROUND:Dendritic cells are characterized by shape, structure, and membrane molecule expression; they contact T lymphocytes to present antigens and stimulate plasma cell differentiation in vitro. Dendritic cells are known to be present in healthy human gingiva and to be altered in HIV-associated periodontitis. Here,...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Cirrincione C,Pimpinelli N,Orlando L,Romagnoli P

    更新日期:2002-01-01 00:00:00

  • Interleukin-1β Gene Expression in Human Oral Polymorphonuclear Leukocytes.

    abstract::Oral polymorphonuclear leukocytes (pmn) were obtained from 10 adult donors in good oral health using a method employing repeated mouth rinse collection. Interleukin-1β (IL-1β) mRNA was detected in freshly obtained cells by blot hybridization of total cellular RNA with a biotin labeled cDNA probe. Supernates from oral ...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Hendley TM,Steed RB,Galbraith GMP

    更新日期:1995-09-01 00:00:00

  • Self-Reported Periodontal Disease: Validation in an Epidemiological Survey.

    abstract:BACKGROUND:Evidence is accumulating to support poor oral health as a risk factor for systemic conditions, including cardiovascular diseases, diabetes control, adverse pregnancy outcomes, and pneumonia. Prohibitive costs for clinical assessment of periodontal disease limit information to assess the prevalence and trends...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Taylor GW,Borgnakke WS

    更新日期:2007-07-01 00:00:00

  • The influence of genetic variation on the splenic T cell cytokine and specific serum antibody responses to Porphyromonas gingivalis in mice.

    abstract:BACKGROUND:T cell cytokine profiles in the spleens and anti-Porphyromonas gingivalis antibodies in the sera of P. gingivalis-immunized BALB/c (H-2d), CBA/CaH (H-2k), C57BL6 (H-2b), and DBA/2J (H-2d, C5 deficient) mice were examined. METHODS:Mice were immunized either by intraperitoneal injections of P. gingivalis oute...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Gemmell E,Winning TA,Grieco DA,Bird PS,Seymour GJ

    更新日期:2000-07-01 00:00:00

  • Biomarkers and Bacteria Around Implants and Natural Teeth in the Same Individuals.

    abstract:BACKGROUND:This cross-sectional study assesses cytokine levels in peri-implant crevicular fluid (PICF)/gingival crevicular fluid (GCF) and a selection of subgingival/submucosal plaque bacteria from clinically healthy or diseased sites in the same individuals. METHODS:Samples from 97 implants/teeth (58 implants [19 hea...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Gürlek Ö,Gümüş P,Nile CJ,Lappin DF,Buduneli N

    更新日期:2017-08-01 00:00:00

  • Transforming Growth Factor-β1 Accelerates Resorption of a Calcium Carbonate Biomaterial in Periodontal Defects.

    abstract:BACKGROUND:In a previous study, recombinant human transforming growth factor-beta1 (rhTGF-β1 ) in a calcium carbonate carrier was implanted into critical-size, supraalveolar periodontal defects under conditions for guided tissue regeneration (GTR) to study whether rhTGF-β1 would enhance or accelerate periodontal regene...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Koo KT,Susin C,Wikesjö UME,Choi SH,Kim CK

    更新日期:2007-04-01 00:00:00

  • Use of furcal bone sounding to improve accuracy of furcation diagnosis.

    abstract::Accurate diagnosis of periodontal destruction in the furcation region of multi-rooted teeth is a critical component of treatment planning, with different therapeutic approaches chosen based upon clinical determination of the severity of involvement. The current study assessed both vertical and horizontal depths of 274...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Mealey BL,Neubauer MF,Butzin CA,Waldrop TC

    更新日期:1994-07-01 00:00:00

  • Inhibitory effects of areca nut extracts on phagocytosis of Actinobacillus actinomycetemcomitans ATCC 33384 by neutrophils.

    abstract:BACKGROUND:Areca quid chewers have a higher prevalence of periodontal disease than non-chewers. Little is known about the influence of areca quid on the immune system. This study was to determine the possible effects of the areca nut on phagocytic activity of human neutrophils. METHODS:Aqueous extracts of ripe areca n...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Hung SL,Cheng YY,Peng JL,Chang LY,Liu TY,Chen YT

    更新日期:2005-03-01 00:00:00

  • Tissue response to titanium implants in the rat maxilla: ultrastructural and histochemical observations of the bone-titanium interface.

    abstract:BACKGROUND:The detailed mechanism of osseointegration, the most appropriate implant-bone interface, remains unclear in jaw tissues at the ultrastructural level in contrast to the many reports using long bones. The present study reports on tissue response to titanium-implantation on an animal model using rat maxilla. M...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Futami T,Fujii N,Ohnishi H,Taguchi N,Kusakari H,Ohshima H,Maeda T

    更新日期:2000-02-01 00:00:00

  • Radiographic methods for the detection of progressive alveolar bone loss.

    abstract::Intraoral transmission radiographs have been the primary diagnostic method for the assessment of bone support as well as for the detection and measurement of osseous changes due to periodontitis. The purpose of the present paper is three-fold. The first is to review radiographic techniques for the assessment of period...

    journal_title:Journal of periodontology

    pub_type: 杂志文章,评审


    authors: Jeffcoat MK

    更新日期:1992-04-01 00:00:00

  • Effect of citric acid treatment on the migration of epithelium on root surfaces in vitro.

    abstract::Explants of bovine gingival mucosa were cultured for four days on scaled and citric acid-conditioned root surfaces. Demineralization of the hard tissue with citric acid exposed the collagenous matrix of the root. Undemineralized islands were frequently seen among the collagen fibers of the treated roots. When cultured...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Larjava H,Salonen J,Häkkinen L,Närhi T

    更新日期:1988-02-01 00:00:00

  • Attachment of Porphyromonas gingivalis to corroded commercially pure titanium and titanium-aluminum-vanadium alloy.

    abstract:BACKGROUND:Titanium dental material can become corroded because of electrochemical interaction in the oral environment. The corrosion process may result in surface modification. It was hypothesized that a titanium surface modified by corrosion may enhance the attachment of periodontal pathogens. This study evaluates th...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Barão VA,Yoon CJ,Mathew MT,Yuan JC,Wu CD,Sukotjo C

    更新日期:2014-09-01 00:00:00

  • Rapid progression of bone loss in HIV-associated necrotizing ulcerative stomatitis.

    abstract::This report describes a case of rapidly progressive periodontal tissue breakdown and bone loss in an HIV-infected markedly immunosuppressed homosexual male. Within 6 months of initial presentation with a necrotizing ulcerative gingivitis, the lesion extended to a necrotizing ulcerative stomatitis involving the surroun...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Patton LL,McKaig R

    更新日期:1998-06-01 00:00:00

  • Lymphocyte numbers and function in relation to periodontitis and smoking.

    abstract:BACKGROUND:T and B lymphocytes play important roles in periodontitis. Smoking is considered a risk factor for periodontitis and may exert its negative effects through leukocytes. Taking smoking into consideration, the aim of this study was to analyze numbers of circulating T (CD3+) cells and their CD4+ and CD8+ subpopu...

    journal_title:Journal of periodontology

    pub_type: 杂志文章


    authors: Loos BG,Roos MT,Schellekens PT,van der Velden U,Miedema F

    更新日期:2004-04-01 00:00:00