Abstract:
BACKGROUND:The clinical use of an enamel matrix derivative (EMD) has been shown to promote formation of new cementum, periodontal ligament (PDL), and bone and to significantly enhance the clinical outcomes after regenerative periodontal surgery. It is currently unknown to what extent the bleeding during periodontal surgery may compete with EMD adsorption to root surfaces. The aim of this study is to evaluate the effect of blood interactions on EMD adsorption to root surfaces mimicking various clinical settings and to test their ability to influence human PDL cell attachment and proliferation. METHODS:Teeth extracted for orthodontic reasons were subjected to ex vivo scaling and root planing and treated with 24% EDTA, EMD, and/or human blood in six clinically related settings to determine the ability of EMD to adsorb to root surfaces. Surfaces were analyzed for protein adsorption via scanning electron microscopy and immunohistochemical staining with an anti-EMD antibody. Primary human PDL cells were seeded on root surfaces and quantified for cell attachment and cell proliferation. RESULTS:Plasma proteins from blood samples altered the ability of EMD to adsorb to root surfaces on human teeth. Samples coated with EMD lacking blood demonstrated a consistent even layer of EMD adsorption to the root surface. In vitro experiments with PDL cells demonstrated improved cell attachment and proliferation in all samples coated with EMD (irrespective of EDTA) when compared to samples containing human blood. CONCLUSION:Based on these findings, it is advised to minimize blood interactions during periodontal surgeries to allow better adsorption of EMD to root surfaces.
journal_name
J Periodontoljournal_title
Journal of periodontologyauthors
Miron RJ,Bosshardt DD,Laugisch O,Katsaros C,Buser D,Sculean Adoi
10.1902/jop.2011.110404subject
Has Abstractpub_date
2012-07-01 00:00:00pages
885-92issue
7eissn
0022-3492issn
1943-3670journal_volume
83pub_type
杂志文章abstract::The research reviewed in this paper constitutes a series of investigations intended to develop and evaluate a new membrane technique, which provides improved conditions for osteogenesis during healing of bone defects and restitution of earlier existing bone. The technique has also been shown to aid in bone grafting as...
journal_title:Journal of periodontology
pub_type: 杂志文章,评审
doi:10.1902/jop.1993.64.11s.1116
更新日期:1993-11-01 00:00:00
abstract::Weary-Kindler syndrome is a rare and poorly understood genetic disorder that has manifestations of both epidermolysis bullosa and poikiloderma congenitale. There are approximately 70 cases documented in the past 40 years but no cases appear in the dental literature, although dental findings have been discussed superfi...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1996.67.10.1004
更新日期:1996-10-01 00:00:00
abstract:BACKGROUND:The aim of this study was to compare the clinical outcome of intrabony periodontal defects following treatment with a novel nanocrystalline hydroxyapatite (NHA) paste to open flap debridement. METHODS:Twenty-eight subjects, each displaying one intrabony defect with probing depth (PD) > or =6 mm and radiogra...
journal_title:Journal of periodontology
pub_type: 杂志文章,随机对照试验
doi:10.1902/jop.2008.070378
更新日期:2008-03-01 00:00:00
abstract:BACKGROUND:Little information is available about the effects of the cessation of cigarette smoking on oral health, although cigarette smoking has been shown to be associated with a variety of oral diseases. The aim of this study was to compare periodontal status, salivary proteolytic activity, especially collagenase-2 ...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1999.70.11.1361
更新日期:1999-11-01 00:00:00
abstract::A multitude of new procedures and materials are being tested for their ability to promote periodontal wound healing. This review provides an overview of the most well-accepted methods for evaluating periodontal wound healing procedures/materials. Topics discussed include appropriate patient selection, general principl...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1992.63.12s.1085
更新日期:1992-12-01 00:00:00
abstract:BACKGROUND:Previous studies have demonstrated that enamel matrix derivative (EMD) has the ability to improve clinical parameters when used to treat intraosseous defects. The purpose of the present study was to compare at 12 months postsurgery sites treated with open flap debridement (OFD) alone to those treated with OF...
journal_title:Journal of periodontology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1902/jop.2001.72.1.25
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:The purpose of this clinical study was to evaluate the possible influence of testosterone hormone on common clinical measurements of periodontal disease in men with hypergonadotropic hypogonadism. METHODS:Twenty-four hypergonadotropic hypogonadal men (H) and 24 systemically healthy men (S) were divided into...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2006.050286
更新日期:2006-07-01 00:00:00
abstract:BACKGROUND:Cannabis sativa (marijuana) can interfere with bone physiopathology because of its effect on osteoblast and osteoclast activity. However, its impact on periodontal tissues is still controversial. The present study evaluates whether marijuana smoke affects bone loss (BL) on ligature-induced periodontitis in r...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2011.100362
更新日期:2011-11-01 00:00:00
abstract::To date, there are no ultrastructural studies that have examined untreated chronic periodontal pockets immediately following dental debridement with an oral irrigation device. This study used both scanning electron microscopic and transmission electron microscopic methodology to examine previously untreated human peri...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1988.59.3.155
更新日期:1988-03-01 00:00:00
abstract::In order to compare the effect of partial demineralization with root planing and partial demineralization of cementum with that of dentin on healing, the ultrastructural morphology of the interface between the layer of human periodontal ligament-derived, fibroblast-like cells (HPF) and the treated root surface was stu...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1995.66.4.267
更新日期:1995-04-01 00:00:00
abstract:BACKGROUND:Previous findings of an association between 25-hydroxyvitamin D [25(OH)D] concentrations and periodontal disease may be partially explained by the antimicrobial properties of vitamin D. To the best of the authors' knowledge, no study has investigated the association between 25(OH)D and pathogenic oral bacter...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2013.130518
更新日期:2014-07-01 00:00:00
abstract:BACKGROUND:Bisphosphonates (BPs) are an important group of drugs used for the treatment of metabolic and oncologic pathologies involving the skeletal system. Osteonecrosis of the jaw (ONJ) is a complication observed in patients using oral or intravenous (IV) BPs. It was suggested that all patients undergoing BP therapy...
journal_title:Journal of periodontology
pub_type: 杂志文章,评审
doi:10.1902/jop.2009.090587
更新日期:2010-04-01 00:00:00
abstract::This cross-sectional study examined the gingivitis occurring at puberty in a population of insulin-dependent juvenile diabetics. Seventy-seven children between the ages of 6 and 15 years were examined for gingivitis levels, stages of pubertal maturation and blood levels of glucose and glycosylated hemoglobin. Bacteria...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1983.54.12.714
更新日期:1983-12-01 00:00:00
abstract::A study was done in an attempt to develop a simple test for the severity of periodontal disease. Gingival fluid collected on filter paper was tested for protein content, and the resulting color was compared to standard color filters. Tissue was excised and prepared for histologic examination. The inflammatory cell inf...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1978.49.8.391
更新日期:1978-08-01 00:00:00
abstract::Ceramic grafting material was used to treat a vertical osseous defect associated with a maxillary left lateral incisor tooth. The area was monitored at maintenance appointments. Root resorption was detected radiographically 12 months after grafting. At 18 months, resorption was evident clinically in the presence of mo...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1985.56.7.419
更新日期:1985-07-01 00:00:00
abstract:BACKGROUND:In patients with severe peridontitis, lower incisors are prone to terminal breakdown. This study assessed the longevity of composite-bonded resin/natural teeth (reinforced only with a stainless steel mesh) as replacements for periodontally lost lower incisors. METHODS:Besides the longevity of the restoratio...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1999.70.2.205
更新日期:1999-02-01 00:00:00
abstract:BACKGROUND:Periodontitis is an infection with an episodic pattern of tissue-support destruction. During the generation of a primary CD4(+) T helper 1 (Th1) response, interferon-gamma (IFN-gamma) acts as a positive regulator by selectively inducing Th1 differentiation through increased transcription of T-bet. The aims o...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2009.080287
更新日期:2009-02-01 00:00:00
abstract::A review of the 2006 and past American Dental Association (ADA) studies on the distribution of dentists provides information on the evolving number of periodontists. Despite an overall increase in the number of periodontists, major differences continue in practitioner/population ratios in different geographic areas. A...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2009.080592
更新日期:2009-05-01 00:00:00
abstract:BACKGROUND:Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia (previously T. forsythensis), which are regarded as the principal periodontopathogenic bacteria, exist as a consortium in subgingival biofilms. We aimed to examine quantitative relationships between P. gingivalis, T. denticola, and T. fo...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2008.070398
更新日期:2008-04-01 00:00:00
abstract:BACKGROUND:Some reports suggest that the prevalence and severity of gingival overgrowth (GO) is lower in patients taking tacrolimus (Tcr) than in those taking cyclosporin A (CsA). The present study was conducted to determine the prevalence and severity of GO in a group of renal transplant recipients treated with Tcr in...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2006.050327
更新日期:2006-06-01 00:00:00
abstract::The inter-incisal distance and dimension of the interdental papilla between the mandibular incisors were examined in cyclosporin A (CSA) fed rats over 6 weeks. Rats in the test group received CSA daily in mineral oil by gastric feeding (30 mg/kg body weight); the control group received mineral oil only. The inter-inci...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1997.68.12.1231
更新日期:1997-12-01 00:00:00
abstract:BACKGROUND:The analysis of samplings from periodontal pockets is important in the diagnosis and therapy of periodontitis. In this study, three different sampling techniques were compared to determine whether one method yielded samples suitable for the reproducible and simultaneous determination of bacterial load, cytok...
journal_title:Journal of periodontology
pub_type: 杂志文章,随机对照试验
doi:10.1902/jop.2011.100565
更新日期:2011-07-01 00:00:00
abstract::Strict control of dental plaque accumulation remains the cornerstone of successful maintenance of the treated patient. However, some recurrences of periodontitis appear to take place despite good oral hygiene habits. A proportion of these are likely due to qualitative features of the periodontal microbiota rather than...
journal_title:Journal of periodontology
pub_type: 杂志文章,评审
doi:10.1902/jop.1988.59.7.439
更新日期:1988-07-01 00:00:00
abstract:BACKGROUND:A 43-year-old African American male initially presented for a dental evaluation of a recurrent swelling on the buccal aspect of tooth #12. His medical history was unremarkable except for a 20-pack year history of smoking. He was eventually diagnosed as having a necrotic pulp #12, and received root canal trea...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2010.090418
更新日期:2010-05-01 00:00:00
abstract::A new technique for the removal of phenytoin hyperplasia using the carbon dioxide (CO2) surgical laser is presented as well as a review of laser physics and the current uses of the CO2 laser in dentistry. Twelve cases of phenytoin hyperplasia removed surgically by the CO2 laser are presented; hence a laser gingivectom...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1985.56.8.492
更新日期:1985-08-01 00:00:00
abstract:BACKGROUND:Valuable decision making for periodontal treatment success in situations where there is ambiguous or conflicting information was previously reported using a consensus building method, the Delphi survey, for posterior teeth with chronic periodontitis. This study focuses on outcome measures relevant in assessi...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2005.76.9.1508
更新日期:2005-09-01 00:00:00
abstract::Extensive data collected over the past decade demonstrate clearly that diseaseactive and disease-inactive periodontal pockets exist, disease progression is infrequent and episodic, and most progression occurs in a small proportion of highly susceptible individuals. Furthermore, traditionally used diagnostic procedures...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1992.63.4s.356
更新日期:1992-04-01 00:00:00
abstract::A new flap design for placement of implants into osseous defects has been described. The flap design can be used in anterior and posterior areas of human subjects. Photographs of representative cases are presented. Wound healing always occurred by primary intention and without evidence of immediate graft exfoliation. ...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1985.56.4.204
更新日期:1985-04-01 00:00:00
abstract::Explants of bovine gingival mucosa were cultured for four days on scaled and citric acid-conditioned root surfaces. Demineralization of the hard tissue with citric acid exposed the collagenous matrix of the root. Undemineralized islands were frequently seen among the collagen fibers of the treated roots. When cultured...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1988.59.2.95
更新日期:1988-02-01 00:00:00
abstract:BACKGROUND:This article reports a rare case of metastasis of salivary duct carcinoma of the parotid gland to the gingiva and reviews the occurrence of metastatic processes to the oral mucosa. METHODS:A 67-year-old white male presented with a chief complaint of a painless nodular tissue growth on the gingiva with repor...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2008.070283
更新日期:2008-04-01 00:00:00