Abstract:
:Malaria is caused by five species of Plasmodium in humans. Microscopy is currently used for pathogen detection, requiring considerable training and technical expertise as the parasites are often difficult to differentiate morphologically. Rapid diagnostic tests are as reliable as microscopy and offer faster diagnoses but possess lower detection limits and are incapable of distinguishing among the parasitic species. To improve global health efforts towards malaria control, a rapid, sensitive, species-specific, and economically viable diagnostic method is needed. In this study, we designed a malaria diagnostic method involving a multiplex single-tube nested PCR targeting Plasmodium mitochondrial cytochrome c oxidase III and single-stranded tag hybridization chromatographic printed-array strip. The detection sensitivity was found to be at least 40 times higher than that of agarose gel electrophoresis with ethidium bromide. This system also enables the identification of both single- and mixed-species malaria infections. The assay was validated with 152 Kenyan samples; using nested PCR as the standard, the assay's sensitivity and specificity were 88.7% and 100.0%, respectively. The turnaround time required, from PCR preparation to signal detection, is 90min. Our method should improve the diagnostic speed, treatment efficacy, and control of malaria, in addition to facilitating surveillance within global malaria eradication programs.
journal_name
Parasitol Intjournal_title
Parasitology internationalauthors
Saito T,Kikuchi A,Kaneko A,Isozumi R,Teramoto I,Kimura M,Hirasawa N,Hiratsuka Mdoi
10.1016/j.parint.2018.01.005subject
Has Abstractpub_date
2018-06-01 00:00:00pages
277-283issue
3eissn
1383-5769issn
1873-0329pii
S1383-5769(17)30377-Xjournal_volume
67pub_type
杂志文章abstract::Human fascioliasis cases in Japan have been reported infrequently and the most appropriate therapy for this disease remains to be determined. This case report describes a patient with the infection unsuccessfully treated with multiple high doses of praziquantel for over 1 year and eventually cured by the administratio...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/s1383-5769(02)00004-1
更新日期:2002-06-01 00:00:00
abstract::Malaria is a disease caused by Plasmodium parasites that affects hundreds of millions of people. Plasmodium proteases are involved in invasion, erythrocyte egress and degradation of host proteins. Falcipains are well-studied cysteine peptidases located in P. falciparum food vacuoles that participate in hemoglobin degr...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2017.12.005
更新日期:2018-04-01 00:00:00
abstract::Taeniasis/cysticercosis has been reported from several provinces of Indonesia: Papua (=former Irian Jaya), Bali, North Sumatra, East Nusa Tenggara, South East Sulawesi, Lampung, North Sulawesi, Jakarta, West Kalimantan, and East Java. The highest level of endemicity of taeniasis/cysticercosis has been found in Papua. ...
journal_title:Parasitology international
pub_type: 杂志文章,评审
doi:10.1016/j.parint.2005.11.025
更新日期:2006-01-01 00:00:00
abstract::Some historical remarks are made on the background information leading to the international symposium on taeniasis/cysticercosis and echincoccosis with focus on Asia and the Pacific. ...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2005.11.002
更新日期:2006-01-01 00:00:00
abstract::Based on specimens from the gill cavities of one Platichthys stellatus individual collected in the Sea of Japan, we investigated the taxonomic status of the enigmatic caligid genus Pseudolepeophtheirus and its type species, Pseudolepeophtheirus longicauda. In a maximum likelihood (ML) tree based on 18S rRNA gene seque...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2020.102135
更新日期:2020-10-01 00:00:00
abstract::Three different monoclonal antibodies were produced against Trypanosona cruzi proteasomes. These antibodies were shown to react with a single 27-kDa band on immunoblots of purified proteasomes. Using a 7E5 monoclonal antibody (IgG1) that recognized the alpha5 subunit of protozoan protease we have studied the intracell...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2009.07.012
更新日期:2009-12-01 00:00:00
abstract::The advances in microscopy combined to the invaluable progress carried by the utilization of molecular, immunological or immunochemical markers and the implementation of more powerful imaging technologies have yielded great improvements to the knowledge of the interaction between microorganisms and their hosts, notabl...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2013.07.011
更新日期:2014-02-01 00:00:00
abstract::Two hyperapolytic species of Acanthobothrium Blanchard, 1848 have been collected from Discopyge tschudii Heckel, 1846 and Zapteryx brevirostris (Müller et Henle) along the coast of Argentina. Acanthobothrium stefaniae sp. n. from D. tschudii is a category one species (i.e., it is less than 15 mm in total length, posse...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2018.04.001
更新日期:2018-08-01 00:00:00
abstract::Tick-borne protozoan and rickettsial diseases are a major threat to livestock in tropical and sub-tropical regions of Africa. In this study we investigated the presence and distribution of Theileria spp., Babesia ovis, Anaplasma ovis, Anaplasma phagocytophilum, Ehrlichia ruminantium and SFG Rickettsia in sheep and goa...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2017.11.002
更新日期:2018-04-01 00:00:00
abstract::The third-stage (L3) larvae of Anisakis, which are the etiological agents of human anisakiasis, have been categorized morphologically into Anisakis Type I larvae and Anisakis Type II larvae. Genetic analysis has allowed easy identification of these larvae: Anisakis Type I larvae include the species Anisakis simplex se...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2011.02.008
更新日期:2011-06-01 00:00:00
abstract::The pathogen Borrelia burgdorferi causes Lyme Borreliosis in human and animals world-wide. In Europe the pathogen is transmitted to the host by the vector Ixodes ricinus. The nymph is the primary instar for transmission to humans. We here study the infection rate of five Borrelia genospecies: B. burgdorferi sensu stri...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2007.07.004
更新日期:2008-03-01 00:00:00
abstract::The asexual erythrocytic cycle of the protozoan parasite Plasmodium falciparum is responsible for the pathogenesis of malaria and causes the overwhelming majority of malaria deaths. Rapidly increasing parasitaemia during this 48hour cycle threatens the survival of the human host and the parasite prior to transmission ...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2016.07.007
更新日期:2016-12-01 00:00:00
abstract::Infections with parasites, such as Strongyloides stercoralis, typically cause elevated levels of serum immunoglobulin E (IgE) and eosinophils; however, co-infection with human T cell lymphotropic virus type 1 (HTLV-1) can cause lower levels of serum IgE during S. stercoralis infection. We conducted this study to deter...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2016.10.004
更新日期:2017-02-01 00:00:00
abstract::The male of Phlebotomus (Larroussius) betisi is described from Malayan caves. Several males have been caught in association with P. betisi females. Males and females have been associated by ecology, biogeography, morphology and molecular biology (homology of the ND4 mtDNA sequences). ...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2008.01.003
更新日期:2008-09-01 00:00:00
abstract::In murine leishmaniasis, the induction of the T-helper type 1 (Th1) response contributes to infection resistance, whereas the establishment of the Th2 response makes the mice susceptible to infection. Interleukin-12 (IL-12) plays a pivotal role in the diversification of immune responses to the Th1 type. In this study,...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/s1383-5769(01)00070-8
更新日期:2001-07-01 00:00:00
abstract::We describe the cloning of a full length calpain-encoding cDNA constructed from two truncated cDNAs isolated from a cDNA library prepared with mRNA isolated from adult worms of the Philippine strain of Schistosoma japonicum. The cDNA sequence is 2.456 kb in length and predicts a protein of 758 residues with a molecula...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/s1383-5769(99)00021-5
更新日期:2000-01-01 00:00:00
abstract::Neosporosis caused by Neospora caninum is a serious disease in cattle and dogs worldwide. It is the major cause of abortion and neonatal mortality in cattle. In this study, we evaluated the anti-N. caninum activity of Chinese medicine extracts (curcumin, artemether), herbicides (atrazine, glyphosate), anticoccidiosis ...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2015.08.002
更新日期:2015-12-01 00:00:00
abstract::Brazilian sardine is emerging as a promising species in Aquaculture. This study describes for the first time a case of parasitic infestation by Amyloodinium in Brazilian sardines Sardinella brasiliensis obtained from natural spawning in captivity. The sardines kept in nurseries were naturally parasitized by the amylod...
journal_title:Parasitology international
pub_type:
doi:10.1016/j.parint.2020.102091
更新日期:2020-06-01 00:00:00
abstract::Paragonimiasis is a typical food-borne parasitic disease, endemic in most parts of Asia, with sporadic case reports from American and African countries. The major source of infection is undercooked freshwater crab or crayfish, though consumption of wild boar meat is also responsible for the infection in Japan, because...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2016.09.009
更新日期:2016-12-01 00:00:00
abstract::Except for imported cases, we have had no new Schistosoma japonicum infection in Japan since 1977. But there are still two habitats of the intermediate snail host: Oncomelania nosophora in the previous endemic areas of Kofu Basin and Obitsu. O. nosophora from Kofu Basin and Obitsu are susceptible to Chinese and Philip...
journal_title:Parasitology international
pub_type: 杂志文章,评审
doi:10.1016/s1383-5769(03)00058-8
更新日期:2003-12-01 00:00:00
abstract::Due to the increasing development of anthelmintic resistance in nematodes worldwide, it is important to search for anthelmintic compounds with new modes of action and also to investigate the possibility to combine compounds with possible synergistic effects. There might also be the chance to take advantage of the fact...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2013.05.006
更新日期:2013-12-01 00:00:00
abstract::Coding regions of double stranded RNA molecules from 3 human faecal samples containing Cryptosporidium hominis, C. felis and C. meleagridis were characterised by sequencing and compared with that previously obtained for C. parvum. Sequences outside the coding regions were also obtained. Overall similarities of between...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2006.06.006
更新日期:2006-12-01 00:00:00
abstract::The antimalarial activity of the hot-water extract of Hydrangea macrophylla var. Otaksa leaves was evaluated against Plasmodium yoelii 17XL in mice. Non-treated control mice died from 6 to 7 days after infection, but mice treated with the leaf extract survived during the experiment. Mice given the extract orally showe...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/s1383-5769(00)00069-6
更新日期:2001-03-01 00:00:00
abstract::The complete mitochondrial (mt) genome sequence of Triodontophorus brevicauda, an intestinal equine nematode parasite was determined for the first time. The circular T. brevicauda mt genome is 14,305 bp in length and contains 36 genes, of which 12 code for protein, 22 for transfer RNA, and two for ribosomal RNA, and l...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2015.06.006
更新日期:2015-10-01 00:00:00
abstract::Hemoplasmosis caused by Mycoplasma spp. have been associated with major economic losses in the global dairy production. Hemoplasma studies in the Philippines are limited despite its potential impact. This study mainly aimed to detect the presence of hemoplasma species in dairy water buffaloes and cattle and know their...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2019.02.004
更新日期:2019-06-01 00:00:00
abstract::The var genes encode a family of adhesion receptor proteins, Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1), which profoundly influence malaria pathogenesis. Only a single var gene is transcribed and one PfEMP1 expressed per P.falciparum parasite. Here we present the in vivo transcript distribution of v...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2009.07.004
更新日期:2009-12-01 00:00:00
abstract::Haintestinum amplum n. g., n. sp. is described from the scrawled cowfish, Acanthostracion quadricornis, collected in the Eastern Gulf of Mexico off Florida, USA. The new species is relatively large and shares characters of the Apocreadiidae and Megaperidae but conforms to the diagnosis of neither. It belongs in a new ...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2013.11.007
更新日期:2014-04-01 00:00:00
abstract::Bovine trypanosomosis is a problem in the livestock industry in Nigeria. A longitudinal survey of cattle sampled during the wet and dry seasons was conducted from April 2016 to March 2017. Blood samples were collected by random sampling from 745 cattle in southwest Nigeria and screened for trypanosomes by internal tra...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2018.09.005
更新日期:2019-02-01 00:00:00
abstract::The association between liver fluke infection caused by Opisthorchis viverrini and cholangiocarcinoma (CCA - hepatic cancer of the bile duct epithelium) has been well established. Multiple mechanisms play a role in the development of CCA, but the excretory/secretory products released by O. viverrini (OvES) represent t...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2016.02.001
更新日期:2017-08-01 00:00:00
abstract::To verify the hypothesis that different pathology of Clonorchis sinensis infection by mouse strains is determined by different responses of cytokines and chemokines, we compared those responses of FVB with those of BALB/c mice. All of FVB mice infected with 30 metacercariae of C. sinensis showed cystic dilatation in t...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2011.07.001
更新日期:2012-03-01 00:00:00