Abstract:
:1. Renin mRNA is present in the kidney and, in lower concentrations, in many extrarenal tissues and serves as an index of renin gene activity, as well as potential renin or prorenin synthesis in cell populations within those tissues. Unfortunately the quantity can be very low. 2. A new, highly sensitive technique is described for detection of renin mRNA that involves the enzyme Taq polymerase for both reverse transcription of renin mRNA into renin cDNA and for amplification of the 769-1099 nucleotide segment by the polymerase chain reaction (PCR), all of which involves a single reaction mixture. 3. In this way renin mRNA was detected in kidney and several extrarenal tissues as a PCR product of approximately 330 base pairs on agarose gels by ethidium-bromide staining or hybridization probing. The region of renin mRNA chosen for amplification spanned several intron sites in the coding sequence so that the presence of amplicants derived from genomic DNA could be readily discriminated, as a band of approximately 1.5 kilobases. 4. Thus single-step PCR offers a powerful new approach to detection of renin mRNA.
journal_name
Clin Exp Pharmacol Physioljournal_title
Clinical and experimental pharmacology & physiologyauthors
Lou YK,Smith DL,Robinson BG,Morris BJdoi
10.1111/j.1440-1681.1991.tb01463.xsubject
Has Abstractpub_date
1991-05-01 00:00:00pages
357-62issue
5eissn
0305-1870issn
1440-1681journal_volume
18pub_type
杂志文章abstract::1. We set out to investigate whether the administration of naloxone alone, naloxone plus vasopressin (AVP) or naloxone plus alprazolam to patients with Cushing's syndrome would result in a blunted dynamic response of the pituitary-adrenal axis compared with normal volunteers. Cushing's syndrome is often difficult to d...
journal_title:Clinical and experimental pharmacology & physiology
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doi:10.1111/j.1440-1681.1996.tb02785.x
更新日期:1996-06-01 00:00:00
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journal_title:Clinical and experimental pharmacology & physiology
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doi:10.1111/j.1440-1681.1989.tb01614.x
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abstract::1. It is a central thesis of this review that in clinical and experimental pharmacology and physiology the goal of statistical analysis should be to minimize the risk of making any false-positive inferences from the results of an experiment (experimentwise Type I error). 2. It is common in clinical and experimental ph...
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doi:10.1111/j.1440-1681.1991.tb01468.x
更新日期:1991-06-01 00:00:00
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更新日期:1990-04-01 00:00:00
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更新日期:2015-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1046/j.1440-1681.2002.03762.x
更新日期:2002-11-01 00:00:00
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journal_title:Clinical and experimental pharmacology & physiology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2014-04-01 00:00:00
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journal_title:Clinical and experimental pharmacology & physiology
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更新日期:1995-01-01 00:00:00
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journal_title:Clinical and experimental pharmacology & physiology
pub_type: 杂志文章
doi:10.1111/1440-1681.13091
更新日期:2019-07-01 00:00:00
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