Abstract：
:Intravital multiphoton microscopy is a powerful tool to study kidney physiology in living animals. However, certain technical issues have curbed its usage to date, including limited depth of tissue penetration and high background emission of endogenous signals. Most previous studies have used the excitation range 700–1000 nm. Since newer longer wavelength excitation lasers may provide solutions to these problems we constructed a microscope coupled to a laser tunable up to 1300 nm and optimized for kidney imaging. This set-up offers substantial advantages for intravital studies, especially when coupled with newly available far-red probes. First, the background at longer wavelengths is markedly reduced, thus increasing the signal to background ratio. Second, the depth of tissue penetration is significantly increased, enabling detailed imaging of previously inaccessible structures, such as deeper glomeruli. Third, using a combination of two- and three-photon excitation, multiple different fluorescent probes can be imaged simultaneously in the same animal, with clear spectral separation. Application of these techniques helped visualize pathological aspects of tubular cell function in a well-established model of acute kidney injury (maleate toxicity). Thus, utilizing long wavelength excitation offers substantial advantages for intravital kidney imaging, which together enhance the capabilities of this powerful and increasingly used research technique.
journal_name
Kidney Intjournal_title
Kidney internationalauthors
Schuh CD,Haenni D,Craigie E,Ziegler U,Weber B,Devuyst O,Hall AMdoi
10.1038/ki.2015.323subject
Has Abstractpub_date
2016-03-01 00:00:00pages
712-9issue
3eissn
0085-2538issn
1523-1755pii
S0085-2538(15)00046-0journal_volume
89pub_type
杂志文章abstract：:Severe sepsis is often accompanied by acute kidney injury (AKI) and albuminuria. Here we studied whether the AKI and albuminuria associated with lipopolysaccharide (LPS) treatment in mice reflects impairment of the glomerular endothelium with its associated endothelial surface layer. LPS treatment decreased the abunda...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2013.286
更新日期：2014-01-01 00:00:00
abstract：:The Standardized Outcomes in Nephrology (SONG) initiative is designed to improve research productivity by building consensus. Stakeholders involved with peritoneal dialysis (PD) have put life participation on top of their list, along with treatment-related infection, cardiovascular complications, and patient and techn...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1016/j.kint.2019.05.002
更新日期：2019-09-01 00:00:00
abstract：:The neonatal proximal tubule has a lower rate of bicarbonate absorption than that of adults. This is due, in part, to a lower rate of apical membrane Na+/H+ antiporter activity. The purpose of these studies was to examine if thyroid hormone could be a factor in the maturational increase in Na+/H+ antiporter activity. ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.1998.00879.x
更新日期：1998-05-01 00:00:00
abstract：:To test the hypothesis that systemic administration of immunoglobulin might reduce glomerular injury in membranous nephropathy through mechanisms involving inhibition of complement activation, we studied the passive Heymann nephritis (PHN) model of membranous nephropathy in rats. The daily administration of immunoglob...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1996.529
更新日期：1996-12-01 00:00:00
abstract：:The purpose of these studies was to test the hypothesis that Randall's plaque develops in unique anatomical sites of the kidney and their formation is conditioned by specific stone-forming pathophysiologies. We performed intraoperative papillary biopsies from kidneys of idiopathic-calcium oxalate (CaOx), intestinal by...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/sj.ki.5000238
更新日期：2006-04-01 00:00:00
abstract：:The natural course of idiopathic membranous nephropathy is variable, with some patients slowly progressing to renal failure while others maintain normal renal function over the entire time. Whether to treat this disease or not is controversial due to the lack of controlled data about the long-term effects of treatment...
journal_title：Kidney international
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1038/ki.1995.453
更新日期：1995-11-01 00:00:00
abstract：:Monoclonal antibodies (mAb) reactive against complement components involved in the classical activation pathway were applied in an indirect immunoperoxidase technique for the histological study of normal and diseased human renal tissues. Prominent staining with antibodies against the C4d fragment was seen in all glome...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1989.302
更新日期：1989-12-01 00:00:00
abstract：:Hyperhomocysteinemia, an arteriosclerotic risk factor, persists in 75% of dialysis patients despite routine low dose supplementation with the B-vitamin co-factors/substrates for homocysteine (Hcy) metabolism, and normal or supernormal plasma status of these vitamins (Atherosclerosis 114:93, 1995). We conducted a place...
journal_title：Kidney international
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1038/ki.1996.19
更新日期：1996-01-01 00:00:00
abstract：:We previously demonstrated that urinary endothelin excretion is increased in rats with extensive renal mass reduction, a model of progressive renal disease. Here we explored whether the increased urinary endothelin in this model were due to induction of renal pre-pro-endothelin-1 gene and whether changes in endothelin...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1993.53
更新日期：1993-02-01 00:00:00
abstract：BACKGROUND:Although atherosclerotic renovascular disease is increasingly recognized in chronic kidney disease, few national level studies have examined its clinical epidemiology. METHODS:Claims data from a 5% random sample of the United States Medicare population were used to select patients without atherosclerotic re...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1111/j.1523-1755.2005.00406.x
更新日期：2005-07-01 00:00:00
abstract：BACKGROUND:Aldosterone has been suggested to play a role in the initiation and progression of diabetic nephropathy. Currently recommended treatment with angiotensin-converting enzyme (ACE) inhibitors or angiotensin II receptor blockers [renin-angiotensin system (RAS) blockade] does not suppress circulating aldosterone ...
journal_title：Kidney international
pub_type： 杂志文章,随机对照试验
doi：10.1111/j.1523-1755.2005.00756.x
更新日期：2005-12-01 00:00:00
abstract：BACKGROUND:Wegener's granulomatosis (WG) is a rare systemic autoimmune disease characterized by small-vessel vasculitis leading to organ damage and the presence of antineutrophil cytoplasmic autoantibodies (ANCAs). ANCAs were shown to be involved in the pathogenesis of the disease by increasing adhesion of polymorphonu...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2000.00430.x
更新日期：2000-12-01 00:00:00
abstract：:We compared the effects of calcitriol and doxercalciferol, in combination with either calcium carbonate or sevelamer, on bone, mineral, and fibroblast growth factor-23 (FGF-23) metabolism in patients with secondary hyperparathyroidism. A total of 60 pediatric patients treated with peritoneal dialysis were randomized t...
journal_title：Kidney international
pub_type： 杂志文章,随机对照试验
doi：10.1038/ki.2010.352
更新日期：2011-01-01 00:00:00
abstract：:Sirtuin 6 (SIRT6) is a NAD+-dependent deacetylase associated with numerous aspects of health and physiology. Overexpression of SIRT6 has emerged as a protector in cardiac tissues against pathologic cardiac hypertrophy. However, the mechanism of this protective effect is not fully understood. Here, both in vivo and in ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2017.10.021
更新日期：2018-04-01 00:00:00
abstract：:The international guideline from Kidney Disease: Improving Global Outcomes suggests a hemoglobin target of 11 to 12 g/dl for children with chronic kidney disease. However, information to support this proposal is very limited in the pediatric population. Because of controversy, the unmet need is the establishment of an...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1016/j.kint.2016.10.006
更新日期：2017-01-01 00:00:00
abstract：:LECT2 amyloidosis is the latest systemic type of amyloidosis to be described. It was discovered in patients with nephrotic syndrome and renal failure and is characterized by amyloid deposition in glomeruli, renal vessels, and interstitium. Clinical and pathological features of earlier phases of this type of amyloidosi...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/ki.2010.18
更新日期：2010-05-01 00:00:00
abstract：:The relationship between leukopenia and the complement system during hemodialysis was re-examined by studying not only the in vivo effects of four different dialyzer membranes (cellulose hydrate, cuprophan, cellulose acetate, and polyacrilonitrile) on leukocyte counts and complement levels, but especially by investiga...
journal_title：Kidney international
pub_type： 临床试验,杂志文章
doi：10.1038/ki.1983.227
更新日期：1983-12-01 00:00:00
abstract：:Donor pretreatment of 100 mg/kg each of cyclophosphamide (CY) and methylprednisolone (P) infused 5 hours before nephrectomy invariably prolongs the survival of DLA mismatched, MLC incompatible nonlittermate Beagle renal allografts as well as the survival of mongrel renal allografts. The effect of donor pretreatment ap...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1982.25
更新日期：1982-02-01 00:00:00
abstract：:[Arg(8)]-vasopressin (AVP) has several functions via its three distinct receptors, V1a, V1b, and V2. The V1a vasopressin receptor (V1aR) is expressed in blood vessels and involved in vascular contraction. Recently, we generated V1a receptor-deficient (V1aR(-/-)) mice and found that they were hypotensive. In addition, ...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/ki.2009.319
更新日期：2009-11-01 00:00:00
abstract：BACKGROUND:Tubulointerstitial inflammation and fibrosis are pathologic hallmarks of end-stage renal disease (ESRD). Here we have used DNA microarray technology to monitor the transcriptomic responses to murine unilateral ureteral obstruction (UUO) with a view to identifying molecular modulators of tubulointerstitial fi...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2003.00306.x
更新日期：2003-12-01 00:00:00
abstract：:Atypical hemolytic uremic syndrome (aHUS) is a rare, genetic, life-threatening disease. The Global aHUS Registry collects real-world data on the natural history of the disease. Here we characterize end-stage renal disease (ESRD)-free survival, the rate of thrombotic microangiopathy, organ involvement and the genetic b...
journal_title：Kidney international
pub_type： 杂志文章,多中心研究
doi：10.1016/j.kint.2018.02.029
更新日期：2018-08-01 00:00:00
abstract：:Defects in the primary cilium/basal body complex of renal tubular cells cause polycystic kidney disease (PKD). To uncover pathways associated with disease progression, we determined the kidney transcriptome of 10-day-old severely and mildly affected cpk mice, a model of recessive PKD. In the severe phenotype, the most...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.5002627
更新日期：2008-01-01 00:00:00
abstract：:The beta-amino acid transport capabilities of rat renal epithelium were assessed using brushborder membrane vesicles (BBMV). Taurine, a metabolically inert sulfur-containing amino acid, was studied with emphasis on the renal adaptation to dietary sulfur amino acid alteration. Three isoproteinic diets were given to Spr...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1983.198
更新日期：1983-11-01 00:00:00
abstract：:Lysophosphatidic acid (LPA) is known to regulate various biological responses by binding to LPA receptors. The serum level of LPA is elevated in diabetes, but the involvement of LPA in the development of diabetes and its complications remains unknown. Therefore, we studied LPA signaling in diabetic nephropathy and the...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2016.11.010
更新日期：2017-06-01 00:00:00
abstract：:The soluble receptor of advanced glycation end product (sRAGE) prevents vascular damage in experimental animal models, and observational studies in the general population support the hypothesis that sRAGE may exert a protective role on the vasculature. To test this in patients with chronic kidney disease, we determine...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2009.419
更新日期：2010-02-01 00:00:00
abstract：:Chronic cyclosporine (CsA) nephrotoxicity is a major complication of heart, bone marrow, and renal transplantation, and is characterized in humans by striped interstitial fibrosis, tubular dilatation and atrophy, and hyalinization of hilar arterioles. This last feature is highly specific for cyclosporine injury and ha...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1995.311
更新日期：1995-08-01 00:00:00
abstract：BACKGROUND:Increased venous pressure (VP) and decreased access flow (Qa) are predictors of dialysis access graft thrombosis. VP is easily obtainable. Qa assessment requires a special device and takes more time. The aims of our randomized multicenter studies were to compare outcome in patients with grafts monitored by V...
journal_title：Kidney international
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1046/j.1523-1755.2001.0590041551.x
更新日期：2001-04-01 00:00:00
abstract：:Recent evidence suggests that glomerular hypertrophy is a key event in the development of focal and segmental glomerulosclerosis and hyalinosis (FSGS) in humans and in many experimental models of FSGS. The initial aim of the present study was to determine if glomerular hypertrophy occurs in a puromycin aminonucleoside...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1996.375
更新日期：1996-09-01 00:00:00
abstract：BACKGROUND:The alteration of renal cell growth is one of the early abnormalities in the diabetic nephropathy. However, the effects of high glucose and its action mechanism in renal proximal tubule cell (PTC) proliferation have not been elucidated. METHODS:The effects of 25 mmol/L glucose on cell proliferation, thymidi...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2001.0590051695.x
更新日期：2001-05-01 00:00:00
abstract：:The ankyrin repeat and sterile α motif (SAM) domain-containing six gene (Anks6) is a candidate for polycystic kidney disease (PKD). Originally identified in the PKD/Mhm(cy/+) rat model of PKD, the disease is caused by a mutation (R823W) in the SAM domain of the encoded protein. Recent studies support the etiological r...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2015.122
更新日期：2015-08-01 00:00:00
