Complete genome sequence of and proposal of Thermofilum uzonense sp. nov. a novel hyperthermophilic crenarchaeon and emended description of the genus Thermofilum.

Abstract:

:A strain of a hyperthermophilic filamentous archaeon was isolated from a sample of Kamchatka hot spring sediment. Isolate 1807-2 grew optimally at 85 °C, pH 6.0-6.5, the parameters being close to those at the sampling site. 16S rRNA gene sequence analysis placed the novel isolate in the crenarchaeal genus Thermofilum; Thermofilum pendens was its closest valid relative (95.7 % of sequence identity). Strain 1807-2 grew organothrophically using polysaccharides (starch and glucomannan), yeast extract or peptone as substrates. The addition of other crenarchaea culture broth filtrates was obligatory required for growth and could not be replaced by the addition of these organisms' cell wall fractions, as it was described for T. pendens. The genome of strain 1807-2 was sequenced using Illumina and PGM technologies. The average nucleotide identities between genome of strain 1807-2 and T. pendens strain HRK 5(T) and "T. adornatus" strain 1910b were 85 and 82 %, respectively. On the basis of 16S rRNA gene sequence phylogeny, ANI calculations and phenotypic differences we propose a novel species Thermofilum uzonense with the type strain 1807-2(T) (= DSM 28062(T) = JCM 19810(T)). Project information and genome sequence was deposited in Genbank under IDs PRJNA262459 and CP009961, respectively.

journal_name

Stand Genomic Sci

authors

Toshchakov SV,Korzhenkov AA,Samarov NI,Mazunin IO,Mozhey OI,Shmyr IS,Derbikova KS,Taranov EA,Dominova IN,Bonch-Osmolovskaya EA,Patrushev MV,Podosokorskaya OA,Kublanov IV

doi

10.1186/s40793-015-0105-y

subject

Has Abstract

pub_date

2015-12-09 00:00:00

pages

122

issn

1944-3277

pii

105

journal_volume

10

pub_type

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