Abstract:
OBJECTIVES:The purpose of this study is to characterize the effect of KAI1 overexpression on the biological behavior of nasopharyngeal carcinoma (NPC) cells. BACKGROUND:Nasopharyngeal carcinoma is a highly malignant tumor with a high rate of incidence in China. Currently, there are no ideal therapeutic options for patients with NPC, but a targeted therapy would have great potential for treating it. Therefore, there is an urgent need for novel therapeutic targets to provide new options for treating NPC. The KAI1 gene was originally identified as a metastasis suppressor gene for advanced human cancer. In NPC cell lines and tissues, the expression of KAI1 decreased as the metastatic potential of cells increased, but its potential as a therapeutic target has not been elucidated. METHODS:Non-transformed nasopharyngeal epithelium cell NP69 and NPC cell line C666-1 were cultured and KAI1 expression in these cells was detected by qRT-PCR and Western blot. After the transfection of KAI1-pCDNA3.1 to NP69 and C666-1, the KAI1 expression in these cells was detected by qRT-PCR and Western blot, the proliferation was performed by MTS, the cell cycle and apoptosis were performed by flow cytometry, the migration and invasion were examined by transwell. RESULTS:Our results showed that KAI1 was significantly upregulated in C666-1 cells compared to that in NP69 cells. In addition, KAI1 overexpression significantly inhibited the proliferation, cell cycle, migration, and invasion, and promoted apoptosis of C666-1 cells, but had no significant effect on NP69 cells. CONCLUSION:Our findings suggest that KAI1 overexpression promotes apoptosis and inhibits proliferation, cell cycle, migration, and invasion in NPC cells. We hypothesize that KAI1 overexpression could be a potential therapeutic target for NPC.
journal_name
Am J Otolaryngoljournal_title
American journal of otolaryngologyauthors
Guo Z,Wang Y,Yang J,Zhong J,Liu X,Xu Mdoi
10.1016/j.amjoto.2016.09.011subject
Has Abstractpub_date
2017-01-01 00:00:00pages
511-517issue
5eissn
0196-0709issn
1532-818Xpii
S0196-0709(16)30208-3journal_volume
38pub_type
杂志文章abstract::This case report describes a patient who was found to have a cerebrospinal fluid (CSF) leak originating from the petrous apex. The patient initially presented with multiple bouts of meningitis. The patient was treated surgically via a middle cranial fossa approach but presented five years later with recurrent meningit...
journal_title:American journal of otolaryngology
pub_type: 杂志文章
doi:10.1016/j.amjoto.2014.02.013
更新日期:2014-05-01 00:00:00
abstract:OBJECTIVE:The aim of the study is to review the management of petrous apex cholesterol granuloma. The surgical approaches for drainage or total removal and the wait and see policy were analyzed, and outcomes were evaluated. METHODS:Retrospective charts of 27 patients managed for petrous apex cholesterol granuloma with...
journal_title:American journal of otolaryngology
pub_type: 杂志文章
doi:10.1016/j.amjoto.2008.08.007
更新日期:2009-11-01 00:00:00
abstract::Melanotic neuroectodermal tumor of infancy is a rare condition that is most commonly found within the head and neck regions. We present a 4-month-old boy with a melanotic neuroectodermal tumor of infancy of his maxilla. Because of the fairly high recurrence rate, the operating microscope was used after surgical excisi...
journal_title:American journal of otolaryngology
pub_type: 杂志文章
doi:10.1053/ajot.2001.20696
更新日期:2001-01-01 00:00:00
abstract:INTRODUCTION:COVID-19 is a pandemic disease known with one of the symptoms is sudden onset anosmia. This symptom sometimes may be the only sign of the disease, therefore it must be research widely. OBJECTIVE:We aim to evaluate odor dysfunction in COVID-19 patients objectively and safely without any risk of transmittin...
journal_title:American journal of otolaryngology
pub_type: 杂志文章
doi:10.1016/j.amjoto.2020.102761
更新日期:2021-01-01 00:00:00
abstract::The laryngeal mask airway (LMA) is regarded as a safer supraglottic airway for general anesthesia compared with conventional endotracheal tubes. We report a case of bilateral vocal cord palsy temporarily observed after using the LMA, which required urgent tracheotomy. Severe swelling of the arytenoid suggested that bi...
journal_title:American journal of otolaryngology
pub_type: 杂志文章,评审
doi:10.1016/j.amjoto.2006.07.001
更新日期:2007-03-01 00:00:00
abstract:OBJECTIVES:Malignant cutaneous adnexal tumors (MCAT) are rare and comprise a heterogeneous group of cancers. There have been several studies reviewing prognostic factors of these tumors, but no studies focusing on the head and neck. This study aimed to review a large population based database to evaluate prognostic fac...
journal_title:American journal of otolaryngology
pub_type: 杂志文章
doi:10.1016/j.amjoto.2018.09.011
更新日期:2019-01-01 00:00:00
abstract::A bilobed free radial forearm flap was designed to reconstruct a defect in the epiglottis and tongue base in 2 patients who underwent supraglottic laryngectomy. The flap was initially sutured in the shape of the epiglottis to prevent aspiration during deglutition. Six months after surgery, after a full course of radia...
journal_title:American journal of otolaryngology
pub_type: 杂志文章
doi:10.1016/j.amjoto.2008.11.004
更新日期:2010-03-01 00:00:00
abstract:OBJECTIVE:This study identifies how recurrent Zenker's diverticula are treated. METHODS:A retrospective chart review was performed from four tertiary referral academic voice and swallowing centers to identify individuals who underwent surgery for recurrent Zenker's diverticulum. Demographic data, surgical modalities f...
journal_title:American journal of otolaryngology
pub_type: 杂志文章
doi:10.1016/j.amjoto.2020.102755
更新日期:2021-01-01 00:00:00
abstract:OBJECTIVES:To evaluate the efficacy of automatic benign paroxysmal positional vertigo (BPPV) diagnosis and treatment system for BPPV compared with the manual repositioning group. METHODS:Two hundred thirty patients diagnosed as idiopathic BPPV who were admitted from August 2018 to July 2019 in Zhejiang Hospital were i...
journal_title:American journal of otolaryngology
pub_type: 杂志文章
doi:10.1016/j.amjoto.2020.102412
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVE:Cochlear nerve preserving translabyrinthine vestibular schwannoma (VS) resection enables concurrent cochlear implantation. Implantation in patients with VS raises important concerns including the ability to undergo postoperative magnetic resonance imaging (MRI) monitoring of residual tumor growth or tumor rec...
journal_title:American journal of otolaryngology
pub_type: 杂志文章
doi:10.1016/j.amjoto.2020.102518
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:Pneumocephalus is a rare complication of endoscopic sinus surgery (ESS) and microscopic skull base surgery (MSBS). Postoperatively, patients often present with headache and altered mental status. Unrepaired leaks are associated with an increased risk of ascending meningitis. Standard treatment of pneumocepha...
journal_title:American journal of otolaryngology
pub_type: 杂志文章
doi:10.1016/j.amjoto.2009.02.012
更新日期:2010-07-01 00:00:00
abstract::Olfactory neuroblastoma is a rare malignant tumor of the nasal cavity arising from the olfactory neuroepithelium. It usually presents as a polypoid mass high in the nasal vault including the cribriform plate, superior turbinate, and superior portion of nasal septum. An 89-year-old man visited our office with symptoms ...
journal_title:American journal of otolaryngology
pub_type: 杂志文章
doi:10.1016/j.amjoto.2006.07.013
更新日期:2007-05-01 00:00:00
abstract:PURPOSE:To review rates of post-tonsillectomy hemorrhage (PTH) at a quaternary medical center, including the impact of narcotic versus nonsteroidal anti-inflammatory drug (NSAID) postoperative pain management. MATERIALS AND METHODS:A retrospective review was performed of tonsillectomies conducted at a single instituti...
journal_title:American journal of otolaryngology
pub_type: 杂志文章,评审
doi:10.1016/j.amjoto.2018.03.028
更新日期:2018-01-01 00:00:00
abstract::Ten guinea pigs were irradiated with 30 Gy of x-radiation. Five were killed on the eighth day after irradiation, and the remainder were killed at the sixteenth day after irradiation. At the time of death, examination was made of the ciliary activity and the fine structure of the middle ear mucosa. Serous effusion was ...
journal_title:American journal of otolaryngology
pub_type: 杂志文章
doi:10.1016/s0196-0709(87)80008-x
更新日期:1987-07-01 00:00:00
abstract:OBJECTIVES:To evaluate the role of combined therapy using montelukast and intranasal mometasone furoate compared to intranasal mometasone furoate alone in treatment of adenoid hypertrophy regarding efficacy and recurrence rate. METHODS:The study included 100 children with adenoid hypertrophy, they were randomly assign...
journal_title:American journal of otolaryngology
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.amjoto.2020.102723
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVES:Edema of the arytenoid and wound hematoma are principal causes of inspiratory distress after arytenoid adduction and type I thyroplasty. The purpose of the present study was demonstrate esophageal regurgitation one of the causes of inspiratory distress after thyroplastic surgeries. STUDY DESIGN:Two case rep...
journal_title:American journal of otolaryngology
pub_type: 杂志文章
doi:10.1016/j.amjoto.2006.01.017
更新日期:2006-11-01 00:00:00
abstract::Starting eight days after hatching, chicks received daily subcutaneous injections of streptomycin sulfate, either 400 mg/kg for 30 days or 1,200 mg/kg for 15 days. Randomly selected chicks from each group were killed at intervals during the injection period, and the ampullae were examined for signs of vestibulotoxicit...
journal_title:American journal of otolaryngology
pub_type: 杂志文章
doi:10.1016/s0196-0709(84)80053-8
更新日期:1984-11-01 00:00:00
abstract:PURPOSE:To reach a consensus on the preoperative evaluation and management of intraoperative and postoperative tonsillectomy hemorrhage. METHOD:A survey on the preoperative evaluation and the management of intraoperative and postoperative tonsillectomy hemorrhage was given to one of the faculty members at each of the ...
journal_title:American journal of otolaryngology
pub_type: 杂志文章
doi:10.1016/0196-0709(95)90006-3
更新日期:1995-01-01 00:00:00
abstract::A choristoma is a nonneoplastic proliferation of histologically normal tissue that forms at an abnormal site. It is extremely uncommon in the middle ear space. It appears to be a developmental abnormality and may be associated with abnormalities of adjacent structures. It usually occurs with unilateral conductive hear...
journal_title:American journal of otolaryngology
pub_type: 杂志文章,评审
doi:10.1016/s0196-0709(00)85011-5
更新日期:2000-03-01 00:00:00
abstract::Ectopic thyroid is a rare developmental anomaly. It can be found anywhere between the foramen cecum and the normal position of the thyroid gland. Massive bleeding from an ectopic lingual thyroid is unusual and occasionally fatal. We present a case of a 22-year-old woman who had a large mass at the base of the tongue f...
journal_title:American journal of otolaryngology
pub_type: 杂志文章
doi:10.1053/ajot.2002.123432
更新日期:2002-05-01 00:00:00
abstract::Sarcoidosis is a systemic chronic granulomatous disease of unknown etiology. Although it most commonly affects African-Americans, Scandinavians, and the Irish, individuals of all races and ethnicities are susceptible. The otolaryngologist will most frequently encounter sarcoidosis involving the sinonasal region; howev...
journal_title:American journal of otolaryngology
pub_type: 杂志文章
doi:10.1053/ajot.2002.28783
更新日期:2002-01-01 00:00:00
abstract::Volumetric changes of the middle ear and mastoid were measured monthly by the direct volumetric method in 28 ears from 21 patients (12 children and nine adults) with otitis media with effusion. The mean volumetric increase immediately after the insertion of the tubes was 1.56 ml and was attributed to the volume of eff...
journal_title:American journal of otolaryngology
pub_type: 杂志文章
doi:10.1016/s0196-0709(86)80053-9
更新日期:1986-07-01 00:00:00
abstract:IMPORTANCE:Resident fatigue has become a point of emphasis in medical education and its effects on otolaryngology residents and their patients require further study. OBJECTIVE:The purpose of our study was to evaluate the prevalence and nature of fatigue in otolaryngology residents, evaluate various quality of life mea...
journal_title:American journal of otolaryngology
pub_type: 杂志文章
doi:10.1016/j.amjoto.2016.01.017
更新日期:2016-05-01 00:00:00
abstract:PURPOSE:The dizziness handicap inventory (DHI) is widely used to evaluate self-perceived handicap due to dizziness, and is known to correlate with vestibular function tests in chronic dizziness. However, whether DHI reflects subjective symptoms during the acute phase has not been studied. This study aims to investigate...
journal_title:American journal of otolaryngology
pub_type: 杂志文章,多中心研究
doi:10.1016/j.amjoto.2015.07.011
更新日期:2015-11-01 00:00:00
abstract::This article describes a woman with hemorrhagic telangiectasia, or Osler-Weber-Rendu disease, with recurrent nosebleeds despite prior septal dermoplasty and laser treatments. Telangiectasias were clearly shown at the time of angiography. Embolization of abnormal vessels on both sides resulted in cessation of epistaxis...
journal_title:American journal of otolaryngology
pub_type: 杂志文章
doi:10.1016/0196-0709(95)90047-0
更新日期:1995-03-01 00:00:00
abstract:PURPOSE:It has been reported widely on various methods of repairing scalp avulsion/defect, including anastomotic vessels for total scalp avulsion and dermal grafts (skin grafting, latissimus dorsi or anterior serratus flap, "visor flap" repair.). The long-term retrospective study, however, with large sample size remain...
journal_title:American journal of otolaryngology
pub_type: 杂志文章
doi:10.1016/j.amjoto.2020.102752
更新日期:2021-01-01 00:00:00
abstract::Esthesioneuroblastoma is a rare malignant tumor of undifferentiated neuroectodermal origin. Because these tumors are derived from olfactory epithelium, most cases arise in the superior nasal cavity at the level of the cribriform niche. The development of an esthesioneuroblastoma outside of the region in which olfactor...
journal_title:American journal of otolaryngology
pub_type: 杂志文章
doi:10.1016/j.amjoto.2004.03.002
更新日期:2004-09-01 00:00:00
abstract::Branhamella catarrhalis has gained increasing recognition as a pathogen in the respiratory tract. During the past 18 years, since its transfer from the genus Neisseria, it has been associated with infection in cavities of the respiratory tract (sinuses and middle ear). It has been recognized as playing a role in laryn...
journal_title:American journal of otolaryngology
pub_type: 杂志文章
doi:10.1016/0196-0709(91)90046-i
更新日期:1991-03-01 00:00:00
abstract::Castleman disease is a rare lymphoproliferative disorder with two primary subtypes that vary in presentation and course. Unicentric Castleman disease (UCD) presents as a solitary mass, most commonly in the mediastinum, and rarely in the head and neck. In contrast to multicentric Castleman disease (MCD), which features...
journal_title:American journal of otolaryngology
pub_type: 杂志文章,评审
doi:10.1016/j.amjoto.2012.11.007
更新日期:2013-05-01 00:00:00
abstract:PURPOSE:Many head and neck surgical procedures are considered clean-contaminated wounds and antibiotic prophylaxis is recommended. Despite prophylaxis, the incidence of surgical site infections remains significant - especially in the setting of free tissue transfer. The antibiotic course is often of a longer duration a...
journal_title:American journal of otolaryngology
pub_type: 杂志文章
doi:10.1016/j.amjoto.2017.01.009
更新日期:2017-01-01 00:00:00