Abstract：
:A fluorescence enhancement-based immunoassay has been developed for the detection of the polycyclic aromatic hydrocarbons (PAH), benzo[a]pyrene (BaP), in aqueous solutions. The results of this study show that BaP, which inefficiently fluoresces in aqueous solution, displays enhanced fluorescence when bound to the anti-BaP antibody (anti-BaP), as part of a label-free immunoassay system. Binding to anti-BaP results in a 3.12-fold increase in the fluorescence intensity of BaP, which emits at 435 nm when excited at 280 nm, due to the hydrophobic interaction and fluorescence resonance energy transfer (FRET) between antibody and antigen. As result of this phenomenon, the antibody-based fluorescence immunoassay system can be used to detect BaP specifically with a limit of detection (LOD) of 0.06 ng mL(-1). Finally, extraction recoveries of BaP from spiked wheat and barley samples were found to be in the range of 80.5-87.0% and 92.9-92.1%, respectively.
journal_name
Chemospherejournal_title
Chemosphereauthors
Li T,Choi YH,Shin YB,Kim HJ,Kim MGdoi
10.1016/j.chemosphere.2016.01.008subject
Has Abstractpub_date
2016-05-01 00:00:00pages
407-413eissn
0045-6535issn
1879-1298pii
S0045-6535(16)30008-Xjournal_volume
150pub_type
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