Abstract:
:The In-R1-G9 cell line is one of the clones derived from the In-111-R1 hamster insulinoma cell line and produces glucagon. The secretory responses of In-R1-G9 cells were further examined to characterize the nature of the cells. Vincristine had no effect on glucagon secretion and colchicine enhanced glucagon secretion slightly after a short incubation. Two calmodulin inhibitors, trifluoperazine and chlorpromazine, did not affect glucagon secretion. Monensin at 10(-8) M suppressed glucagon secretion by 50%. Secretion of glucagon was calcium-dependent. The addition of A23187 to the incubation medium resulted in a 180% increase over control for 1 h and calcium deprivation from the medium suppressed glucagon secretion markedly. Theophylline, a phosphodiesterase inhibitor, caused a 230% increase in glucagon secretion. An experiment using cycloheximide suggested that newly synthesized glucagon appears in the medium at 30 min. This cell line should be useful for various experiments in many fields of research.
journal_name
Diabetes Res Clin Practjournal_title
Diabetes research and clinical practiceauthors
Ono J,Yamaguchi K,Okeda T,Asano T,Takaki Rdoi
10.1016/s0168-8227(88)80019-6subject
Has Abstractpub_date
1988-02-19 00:00:00pages
203-7issue
3eissn
0168-8227issn
1872-8227pii
S0168-8227(88)80019-6journal_volume
4pub_type
杂志文章abstract:AIMS:To evaluate the incidence of impaired glucose tolerance (IGT), impaired fasting glucose (IFG) and diabetes in 318 Caucasian women with gestational diabetes (GDM) at 6 weeks postpartum. METHODS:All women had 75g OGTT and the following data were collected: age, height, weight, results of the challenge 50g and diagn...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2009.04.003
更新日期:2009-06-01 00:00:00
abstract::Circulating insulin antibodies at birth and the degree of maternal metabolic control were measured in 68 infants of insulin-treated diabetic mothers. Their correlation with neonatal B cell function and with the clinical features of the infants was evaluated in order to better understand their influence on fetal outcom...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/0168-8227(89)90016-8
更新日期:1989-11-06 00:00:00
abstract:AIM:To determine the possible factors predicting the insulin requirement in pregnancies complicated by gestational diabetes mellitus (GDM). METHOD:A total of 294 patients with GDM diagnosed by the 100-g/3-h oral glucose tolerance test (OGTT) were studied. The following factors were analyzed: maternal age, nulliparity,...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2009.12.023
更新日期:2010-04-01 00:00:00
abstract:AIMS:Body-weight fluctuation is associated with an increased risk of all-cause mortality. Yet no studies investigate its association with risk of diabetes in adults aged ≥ 60 years. This study aimed to address this issue. METHODS:A total of 1,565 participants free of diabetes at baseline in the CHARLS were followed fo...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2020.108419
更新日期:2020-11-01 00:00:00
abstract::We assessed the effect of CS-045, a new hypoglycemic agent, on B-cell function in partially pancreatectomized rats. At the age of 4 weeks, male Wistar rats were subjected to 90% pancreatectomy (Px). For 2 weeks starting at 6 weeks after surgery the Px rats were treated with CS-045 (CS rats) mixed with chow pellets in ...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/0168-8227(94)01022-r
更新日期:1995-01-01 00:00:00
abstract:AIMS:Deleterious effects of metabolic syndrome (MS) on bone are still controversial. In this study we evaluated the effects of a fructose-induced MS, and/or an oral treatment with metformin on the osteogenic potential of bone marrow mesenchymal stromal cells (MSC), as well as on bone formation and architecture. METHOD...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2017.02.011
更新日期:2017-04-01 00:00:00
abstract::The increased susceptibility to atherosclerosis of diabetic individuals, may result from diabetes-associated modification in plasma low density lipoproteins (LDL) which enhance their interaction with arterial extracellular matrix proteoglycans. Using a nonhuman primate model for human diabetes, studies were conducted ...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/s0168-8227(99)00074-1
更新日期:1999-10-01 00:00:00
abstract::To critically evaluate the relative importance of coagulation abnormalities over other clinical variables for micro- and macrovascular diabetic complications, prothrombin fragment (F1+2), thrombin-antithrombin III complex (TAT), fibrin degradation product d-dimer, and alpha2 plasmin inhibitor complex were determined i...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/s0168-8227(00)00134-0
更新日期:2000-07-01 00:00:00
abstract:AIMS:We tested the hypothesis that short sleep duration is not only a risk factor for diabetes, but that the two conditions in combination would be associated with the risk of incident cardiovascular disease (CVD). METHODS:We analyzed 1255 hypertensive patients (mean age: 70.4±9.9 years) with (N=299) and without diabe...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2012.09.014
更新日期:2012-12-01 00:00:00
abstract::In this retrospective study, the prevalence of chronic microangiopathic complications was determined in 474 Chinese patients with non-insulin dependent diabetes mellitus (NIDDM) who presented within 1 year of diagnosis to the diabetes clinic from January 1990 to December 1996. Mean age (+/- S.E.) was 53.6 (+/- 0.6) ye...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/s0168-8227(98)00002-3
更新日期:1998-03-01 00:00:00
abstract:AIM:Whether exposure to relatively high levels of air pollution is associated with diabetes occurrence remains unclear. We sought to assess and quantify the association between exposure to major air pollutants and risk of type 2 diabetes. METHODS:PubMed and EMBASE databases (through September 2013) were searched using...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.diabres.2014.08.010
更新日期:2014-11-01 00:00:00
abstract:AIM:To evaluate perceived risk, control, worry, and severity about diabetes, coronary heart disease (CHD) and stroke among individuals at increased familial risk of diabetes. METHODS:Data analyses were based on the Family Healthware™ Impact Trial. Baseline health beliefs were compared across three groups: (1) no famil...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2011.12.017
更新日期:2012-05-01 00:00:00
abstract:AIMS:Diabetes stigma and weight stigma have been identified as important but neglected issues that warrant attention among people with type 2 diabetes. This study assessed associations of diabetes stigma and weight stigma with diabetes self-care behaviors and health care in adults with type 2 diabetes. METHODS:U.S. ad...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2020.108387
更新日期:2020-10-01 00:00:00
abstract:AIMS:We evaluated whether glucose fluctuation (GF) causes vascular endothelial injury and affects glucometabolic factors during lengthy oral glucose tolerance test (OGTT). METHODS:We enrolled consecutive 116 patients with coronary artery disease (CAD) who were performed coronary angiography and 4-h OGTT. Blood samples...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2017.07.007
更新日期:2017-09-01 00:00:00
abstract::We investigated gender differences in the relationships between the Trp64Arg variant of the beta(3)-adrenergic receptor (AR) gene in obesity and insulin resistance in nondiabetic subjects. In 476 nondiabetic Japanese-Americans (M/F=204/272), the Trp64Arg variant of the beta(3)-AR gene was examined. The presence or abs...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/s0168-8227(01)00284-4
更新日期:2001-10-01 00:00:00
abstract::Increased plasma plasminogen activator inhibitor type 1 (PAI-1), coagulation factor VII (FVII) and fibrinogen levels have been recognized as risk factors for cardiovascular disease. Because a substantially high incidence of cardiovascular disease has been reported in diabetic patients with nephropathy compared with th...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/s0168-8227(97)01384-3
更新日期:1997-04-01 00:00:00
abstract::We measured transhepatic C-peptide and insulin concentrations in plasma, and hepatic removal of insulin, to examine whether the practice of reporting the C-peptide:insulin molar ratio as a measure of the hepatic removal of insulin is valid. In anesthetized dogs (n = 6), during electromagnetic hepatic blood flow monito...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/s0168-8227(87)80031-1
更新日期:1987-11-01 00:00:00
abstract:AIMS:Type 2 diabetes mellitus (T2DM) is associated with subtle cognitive changes, but also with more severe stages of cognitive dysfunction, including mild cognitive impairment (MCI) and dementia. For these severe stages, it is uncertain which domains are primarily affected and if all patients with impairment are captu...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2018.05.025
更新日期:2018-08-01 00:00:00
abstract:AIMS:The present study aimed to investigate the pathophysiological role of SGK1 in the development of metabolic syndrome by investigating the expression and regulation of serum and glucocorticoid-inducible kinase 1 (SGK1) in adipose tissues in obesity and diabetes. METHODS:SGK1 expression in adipose tissue was investi...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2013.08.009
更新日期:2013-10-01 00:00:00
abstract::Epidemiologic studies of migrant populations provide very promising clues towards understanding the roles of genetics and environmental factors in the etiology of diabetes mellitus. Populations of Japanese ancestry are of particular interest due to marked differences in prevalence rates of non-insulin dependent diabet...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:
更新日期:1996-10-01 00:00:00
abstract::The aim of this study was to examine distinctive clinical characteristics of non-insulin dependent diabetes mellitus (NIDDM) patients in Kuwait including mode of presentation at diagnosis, family history of diabetes, therapeutic management and response to treatment. We studied 3299 Kuwaiti patients (1454 male (M) and ...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/0168-8227(95)01128-5
更新日期:1995-08-01 00:00:00
abstract::Activity of the lower leg muscles in response to an unexpected disturbance to upright standing was studied in diabetic patients and non-diabetic matched controls. The diabetic individuals were classified into two groups: diabetic patients with normal cutaneous sensitivity in the foot (n=27) and patients with cutaneous...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/s0168-8227(01)00231-5
更新日期:2001-07-01 00:00:00
abstract::Many studies show poor agreement between fasting plasma glucose (FPG)-based and 2-h postchallenge glucose (2-h PG)-based criteria to assess glucose metabolism. We examined the rate of agreement between FPG- and 2-h PG-based criteria in the diagnosis of intermediate hyperglycemia in four representative cohort studies i...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章,多中心研究
doi:10.1016/j.diabres.2008.01.022
更新日期:2008-06-01 00:00:00
abstract:OBJECTIVE:To investigate association of psychosocial factors with anxiety and depressive symptoms in Chinese type 2 diabetes patients. METHODS:Three hundred and four type 2 diabetes patients were inquired with a structured questionnaire about socio-demographic characteristics, psychological stress, coping styles, soci...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2007.10.014
更新日期:2008-03-01 00:00:00
abstract::Cardiovascular risk reduction in individuals with Type 2 diabetes mellitus (T2DM) is a key part of clinical management. Sodium-glucose co-transporter (SGLT2) inhibitors improve glycaemic control, reduce body weight and decrease blood pressure. In addition, the SGLT2 inhibitors empagliflozin and canagliflozin reduced t...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章,评审
doi:10.1016/j.diabres.2017.11.023
更新日期:2018-02-01 00:00:00
abstract:OBJECTIVE:The aim of the study was to compare pregnancy outcomes with glycemic control, total increase in insulin requirement, and body weight gain in the women with Type 1 Diabetes Mellitus (T1DM) using continuous subcutaneous insulin infusion (CSII) or multiple daily injections (MDI). MATERIAL AND METHODS:This was a...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2020.108628
更新日期:2020-12-22 00:00:00
abstract::Amid the rapidly rising number of people with diabetes worldwide, the prevalence of diabetic kidney disease (DKD) is expected to increase considerably despite available treatments. Consequently, novel therapeutic agents are urgently needed. Ruboxistaurin mesylate is a bisindolylmaleimide that specifically inhibits the...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章,评审
doi:10.1016/j.diabres.2008.09.041
更新日期:2008-11-13 00:00:00
abstract::We examined trends and associated factors of disability among Spanish elderly with diabetes from data of three National Health Surveys (NHS) from 2000 to 2007. Prevalence of disability was higher among diabetic elderly versus non-sufferers and increased over time. Adjusted OR for activities of daily living 1.66 (1.37-...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2011.07.024
更新日期:2011-11-01 00:00:00
abstract:AIM:To evaluate the safety and effectiveness of biphasic insulin aspart 30 (BIAsp 30) in Indonesian patients with type 2 diabetes (T2D) as part of the 24-week, international, prospective, non-interventional A₁chieve study. METHODS:Indonesian patients who started BIAsp 30 were included. Safety and efficacy was measured...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章,多中心研究
doi:10.1016/S0168-8227(13)70011-1
更新日期:2013-04-01 00:00:00
abstract::We investigated how fasting or postprandial insulin levels were altered by treatment with acarbose or sulfonylureas. Plasma glucose and serum insulin, C-peptide, and proinsulin levels were measured before as well as 1 and 2 h after breakfast in 23 patients with non-insulin-dependent diabetes mellitus and 17 patients w...
journal_title:Diabetes research and clinical practice
pub_type: 临床试验,杂志文章
doi:10.1016/s0168-8227(97)00045-4
更新日期:1997-06-01 00:00:00