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Successful chondrogenesis within scaffolds, using magnetic stem cell confinement and bioreactor maturation.

Abstract：

UNLABELLED:Tissue engineering strategies, such as cellularized scaffolds approaches, have been explored for cartilage replacement. The challenge, however, remains to produce a cartilaginous tissue incorporating functional chondrocytes and being large and thick enough to be compatible with the replacement of articular defects. Here, we achieved unprecedented cartilage tissue production into a porous polysaccharide scaffold by combining of efficient magnetic condensation of mesenchymal stem cells, and dynamic maturation in a bioreactor. In optimal conditions, all the hallmarks of chondrogenesis were enhanced with a 50-fold increase in collagen II expression compared to negative control, an overexpression of aggrecan and collagen XI, and a very low expression of collagen I and RUNX2. Histological staining showed a large number of cellular aggregates, as well as an increased proteoglycan synthesis by chondrocytes. Interestingly, electron microscopy showed larger chondrocytes and a more abundant extracellular matrix. In addition, the periodicity of the neosynthesized collagen fibers matched that of collagen II. These results represent a major step forward in replacement tissue for cartilage defects. STATEMENT OF SIGNIFICANCE:A combination of several innovative technologies (magnetic cell seeding, polysaccharide porous scaffolds, and dynamic maturation in bioreactor) enabled unprecedented successful chondrogenesis within scaffolds.

journal_name

Acta Biomater

journal_title

Acta biomaterialia

authors

Luciani N,Du V,Gazeau F,Richert A,Letourneur D,Le Visage C,Wilhelm C

doi

10.1016/j.actbio.2016.04.009

subject

Has Abstract

pub_date

2016-06-01 00:00:00

pages

101-10

eissn

1742-7061

issn

1878-7568

pii

S1742-7061(16)30162-3

journal_volume

37

pub_type

杂志文章

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