Rapid, Multiplexed Characterization of Shiga Toxin-Producing Escherichia coli (STEC) Isolates Using Suspension Array Technology.

Abstract:

:Molecular methods have emerged as the most reliable techniques to detect and characterize pathogenic Escherichia coli. These molecular techniques include conventional single analyte and multiplex PCR, PCR followed by microarray detection, pulsed-field gel electrophoresis (PFGE), and whole genome sequencing. The choice of methods used depends upon the specific needs of the particular study. One versatile method involves detecting serogroup-specific markers by hybridization or binding to encoded microbeads in a suspension array. This molecular serotyping method has been developed and adopted for investigating E. coli outbreaks. The major advantages of this technique are the ability to simultaneously serotype E. coli and detect the presence of virulence and pathogenicity markers. Here, we describe the development of a family of multiplex molecular serotyping methods for Shiga toxin-producing E. coli, compare their performance to traditional serotyping methods, and discuss the cost-benefit balance of these methods in the context of various food safety objectives.

journal_name

Front Microbiol

authors

Carter JM,Lin A,Clotilde L,Lesho M

doi

10.3389/fmicb.2016.00439

subject

Has Abstract

pub_date

2016-05-20 00:00:00

pages

439

issn

1664-302X

journal_volume

7

pub_type

杂志文章,评审
  • Screening of Bifidobacteria and Lactobacilli Able to Antagonize the Cytotoxic Effect of Clostridium difficile upon Intestinal Epithelial HT29 Monolayer.

    abstract::Clostridium difficile is an opportunistic pathogen inhabiting the human gut, often being the aetiological agent of infections after a microbiota dysbiosis following, for example, an antibiotic treatment. C. difficile infections (CDI) constitute a growing health problem with increasing rates of morbidity and mortality ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00577

    authors: Valdés-Varela L,Alonso-Guervos M,García-Suárez O,Gueimonde M,Ruas-Madiedo P

    更新日期:2016-04-22 00:00:00

  • Biological Stability of Drinking Water: Controlling Factors, Methods, and Challenges.

    abstract::Biological stability of drinking water refers to the concept of providing consumers with drinking water of same microbial quality at the tap as produced at the water treatment facility. However, uncontrolled growth of bacteria can occur during distribution in water mains and premise plumbing, and can lead to hygienic ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.00045

    authors: Prest EI,Hammes F,van Loosdrecht MC,Vrouwenvelder JS

    更新日期:2016-02-01 00:00:00

  • The Shift of the Intestinal Microbiome in the Innate Immunity-Deficient Mutant rde-1 Strain of C. elegans upon Orsay Virus Infection.

    abstract::The status of intestinal microbiota is a determinant of host health. However, the alteration of the gut microbiota caused by the innate immune response to virus infection is unclear. Caenorhabditis elegans and its natural virus Orsay provide an excellent model of host-virus interactions. We evaluated the intestinal mi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00933

    authors: Guo Y,Xun Z,Coffman SR,Chen F

    更新日期:2017-05-29 00:00:00

  • Selection of Competitive and Efficient Rhizobia Strains for White Clover.

    abstract::The practice of inoculating forage legumes with rhizobia strains is widespread. It is assumed that the inoculated strain determines the performance of the symbiosis and nitrogen fixation rates. However, native-naturalized strains can be competitive, and actual nodule occupancy is often scarcely investigated. In conseq...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00768

    authors: Irisarri P,Cardozo G,Tartaglia C,Reyno R,Gutiérrez P,Lattanzi FA,Rebuffo M,Monza J

    更新日期:2019-04-23 00:00:00

  • Identification of Novel Long Non-coding and Circular RNAs in Human Papillomavirus-Mediated Cervical Cancer.

    abstract::Cervical cancer is the third most common cancer worldwide and the fourth leading cause of cancer-associated mortality in women. Accumulating evidence indicates that long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) may play key roles in the carcinogenesis of different cancers; however, little is known about ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01720

    authors: Wang H,Zhao Y,Chen M,Cui J

    更新日期:2017-09-19 00:00:00

  • Halotolerant PGPR Stenotrophomonas maltophilia BJ01 Induces Salt Tolerance by Modulating Physiology and Biochemical Activities of Arachis hypogaea.

    abstract::Arachis hypogaea (Peanut) is one of the most important cash crops grown for food and oil production. Salinity is a major constraint for loss of peanut productivity, and halotolerant plant growth promoting bacteria not only enhance plant-growth but also provide tolerance against salt stress. The potential of halotolera...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.568289

    authors: Alexander A,Singh VK,Mishra A

    更新日期:2020-10-14 00:00:00

  • Decontamination of Minimally-Processed Fresh Lettuce Using Reuterin Produced by Lactobacillus reuteri.

    abstract::Over the last years the demand for pre-washed, fresh-cut, and minimally-processed (MP) produce has increased. MP fresh vegetable are rapidly spoiled, whereas there is consumers' concern about chemical disinfection treatments such as with chlorine. A promising antimicrobial is reuterin, a broad-spectrum-antimicrobial c...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01421

    authors: Asare PT,Greppi A,Stettler M,Schwab C,Stevens MJA,Lacroix C

    更新日期:2018-07-04 00:00:00

  • Multiplex detection of nine food-borne pathogens by mPCR and capillary electrophoresis after using a universal pre-enrichment medium.

    abstract::Routine microbiological quality analyses in food samples require, in some cases, an initial incubation in pre-enrichment medium. This is necessary in order to ensure that small amounts of pathogenic strains are going to be detected. In this work, a universal pre-enrichment medium has been developed for the simultaneou...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01194

    authors: Villamizar-Rodríguez G,Fernández J,Marín L,Muñiz J,González I,Lombó F

    更新日期:2015-11-03 00:00:00

  • Defining Nutrient Combinations for Optimal Growth and Polyhydroxybutyrate Production by Methylosinus trichosporium OB3b Using Response Surface Methodology.

    abstract::Methane and methanol are common industrial by-products that can be used as feedstocks for the production of value-added products by methylotrophic bacteria. Alphaproteobacterial methanotrophs are known to produce and accumulate the biopolymer polyhydroxybutyrate (PHB) under conditions of nutrient starvation. The prese...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01513

    authors: Zaldívar Carrillo JA,Stein LY,Sauvageau D

    更新日期:2018-07-18 00:00:00

  • Membrane homeoviscous adaptation in the piezo-hyperthermophilic archaeon Thermococcus barophilus.

    abstract::The archaeon Thermococcus barophilus, one of the most extreme members of hyperthermophilic piezophiles known thus far, is able to grow at temperatures up to 103°C and pressures up to 80 MPa. We analyzed the membrane lipids of T. barophilus by high performance liquid chromatography-mass spectrometry as a function of pr...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01152

    authors: Cario A,Grossi V,Schaeffer P,Oger PM

    更新日期:2015-10-21 00:00:00

  • Nutrient Removal and Uptake by Native Planktonic and Biofilm Bacterial Communities in an Anaerobic Aquifer.

    abstract::Managed aquifer recharge (MAR) offers a collection of water storage and storage options that have been used by resource managers to mitigate the reduced availability of fresh water. One of these technologies is aquifer storage and recovery (ASR), where surface water is treated then recharged into a storage zone within...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01765

    authors: Lisle JT

    更新日期:2020-07-29 00:00:00

  • Fungal Diversity and Mycotoxins in Low Moisture Content Ready-To-Eat Foods in Nigeria.

    abstract::Low moisture content ready-to-eat foods vended in Nigerian markets could be pre-packaged or packaged at point of sale. These foods are widely and frequently consumed across Nigeria as quick foods. Despite their importance in the daily diets of Nigerians, a comprehensive study on the diversity of fungi, fungal metaboli...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00615

    authors: Ezekiel CN,Oyedele OA,Kraak B,Ayeni KI,Sulyok M,Houbraken J,Krska R

    更新日期:2020-04-09 00:00:00

  • Population Genomics of Mycobacterium leprae Reveals a New Genotype in Madagascar and the Comoros.

    abstract::Human settlement of Madagascar traces back to the beginning of the first millennium with the arrival of Austronesians from Southeast Asia, followed by migrations from Africa and the Middle East. Remains of these different cultural, genetic, and linguistic legacies are still present in Madagascar and other islands of t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00711

    authors: Avanzi C,Lécorché E,Rakotomalala FA,Benjak A,Rapelanoro Rabenja F,Ramarozatovo LS,Cauchoix B,Rakoto-Andrianarivelo M,Tió-Coma M,Leal-Calvo T,Busso P,Boy-Röttger S,Chauffour A,Rasamoelina T,Andrianarison A,Sendrasoa F,Spen

    更新日期:2020-05-11 00:00:00

  • Succession of Composition and Function of Soil Bacterial Communities During Key Rice Growth Stages.

    abstract::Elucidating the succession of soil microbial communities and microbial functions at key plant growth stages is a major goal of microbial ecology research. In this study, we investigated the succession of soil bacteria during four fertilizer treatments (control, NPK, NPK + pig manure, and NPK + straw) and at three cruc...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00421

    authors: Wang W,Luo X,Chen Y,Ye X,Wang H,Cao Z,Ran W,Cui Z

    更新日期:2019-03-11 00:00:00

  • Modulation of Litter Decomposition by the Soil Microbial Food Web Under Influence of Land Use Change.

    abstract::Soil microbial communities modulate soil organic matter (SOM) dynamics by catalyzing litter decomposition. However, our understanding of how litter-derived carbon (C) flows through the microbial portion of the soil food web is far from comprehensive. This information is necessary to facilitate reliable predictions of ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02860

    authors: Heijboer A,de Ruiter PC,Bodelier PLE,Kowalchuk GA

    更新日期:2018-11-26 00:00:00

  • Recent Developments of the Synthetic Biology Toolkit for Clostridium.

    abstract::The Clostridium genus is a large, diverse group consisting of Gram-positive, spore-forming, obligate anaerobic firmicutes. Among this group are historically notorious pathogens as well as several industrially relevant species with the ability to produce chemical commodities, particularly biofuels, from renewable bioma...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2018.00154

    authors: Joseph RC,Kim NM,Sandoval NR

    更新日期:2018-02-12 00:00:00

  • Co-expression of Nisin Z and Leucocin C as a Basis for Effective Protection Against Listeria monocytogenes in Pasteurized Milk.

    abstract::Nisin, an important bacteriocin from Lactococcus lactis subsp., is primarily active against various Gram-positive bacteria. Leucocin C, produced by Leuconostoc carnosum 4010, is a class IIa bacteriocin used to inhibit the growth of Listeria monocytogenes. Because two bacteriocins have different modes of action, the co...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00547

    authors: Fu Y,Mu D,Qiao W,Zhu D,Wang X,Liu F,Xu H,Saris P,Kuipers OP,Qiao M

    更新日期:2018-03-23 00:00:00

  • Assessing the advantage of morphological changes in Candida albicans: a game theoretical study.

    abstract::A range of attributes determines the virulence of human pathogens. During interactions with their hosts, pathogenic microbes often undergo transitions between distinct stages, and the ability to switch between these can be directly related to the disease process. Understanding the mechanisms and dynamics of these tran...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00041

    authors: Tyc KM,Kühn C,Wilson D,Klipp E

    更新日期:2014-02-06 00:00:00

  • Programming Bacteria With Light-Sensors and Applications in Synthetic Biology.

    abstract::Photo-receptors are widely present in both prokaryotic and eukaryotic cells, which serves as the foundation of tuning cell behaviors with light. While practices in eukaryotic cells have been relatively established, trials in bacterial cells have only been emerging in the past few years. A number of light sensors have ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2018.02692

    authors: Liu Z,Zhang J,Jin J,Geng Z,Qi Q,Liang Q

    更新日期:2018-11-08 00:00:00

  • Starch Flocculation by the Sweet Potato Sour Liquid Is Mediated by the Adhesion of Lactic Acid Bacteria to Starch.

    abstract::In the current study, we focused on the mechanism underlying starch flocculation by the sweet potato sour liquid. The traditional microbial techniques and 16S rDNA sequencing revealed that Lactobacillus was dominant flocculating microorganism in sour liquid. In total, 86 bacteria, 20 yeasts, and 10 molds were isolated...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01412

    authors: Zhang L,Yu Y,Li X,Li X,Zhang H,Zhang Z,Xu Y

    更新日期:2017-07-25 00:00:00

  • Cholecystectomy Damages Aging-Associated Intestinal Microbiota Construction.

    abstract::The intestinal microbiome is essential in humans to maintain physiological balance and nutrition metabolism. Laparoscopic cholecystectomy due to gallstone disease and cholecystitis can cause intestinal microbial dysbiosis, and following bile acid metabolism dysfunction, positions the patient at high risk of colorectal...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01402

    authors: Wang W,Wang J,Li J,Yan P,Jin Y,Zhang R,Yue W,Guo Q,Geng J

    更新日期:2018-06-25 00:00:00

  • Parasites in algae mass culture.

    abstract::Parasites are now known to be ubiquitous across biological systems and can play an important role in modulating algal populations. However, there is a lack of extensive information on their role in artificial ecosystems such as algal production ponds and photobioreactors. Parasites have been implicated in the demise o...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2014.00278

    authors: Carney LT,Lane TW

    更新日期:2014-06-06 00:00:00

  • Molecular Characteristics of Burkholderia pseudomallei Collected From Humans in Hainan, China.

    abstract::Melioidosis is a common infectious disease in Southeast Asia and Northern Australia. In Hainan, several cases have been reported, but no systematic study has yet been done on the molecular epidemiology profiles of the organism. An investigation of the molecular epidemiology links and population structure of Burkholder...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00778

    authors: Zhu X,Chen H,Li S,Wang LC,Wu DR,Wang XM,Chen RS,Li ZJ,Liu ZG

    更新日期:2020-05-07 00:00:00

  • Challenging Oil Bioremediation at Deep-Sea Hydrostatic Pressure.

    abstract::The Deepwater Horizon accident has brought oil contamination of deep-sea environments to worldwide attention. The risk for new deep-sea spills is not expected to decrease in the future, as political pressure mounts to access deep-water fossil reserves, and poorly tested technologies are used to access oil. This also a...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.01203

    authors: Scoma A,Yakimov MM,Boon N

    更新日期:2016-08-03 00:00:00

  • Alternaria Toxins: Potential Virulence Factors and Genes Related to Pathogenesis.

    abstract::Alternaria is an important fungus to study due to their different life style from saprophytes to endophytes and a very successful fungal pathogen that causes diseases to a number of economically important crops. Alternaria species have been well-characterized for the production of different host-specific toxins (HSTs)...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2017.01451

    authors: Meena M,Gupta SK,Swapnil P,Zehra A,Dubey MK,Upadhyay RS

    更新日期:2017-08-08 00:00:00

  • Intestinal Microbiome-Metabolome Responses to Essential Oils in Piglets.

    abstract::This study investigated the effects of dietary essential oils (EOs) on intestinal microbial composition and metabolic profiles in weaned piglets. The piglets were fed the same basal diet supplemented with EOs (EO) or without EOs (Con) in the current study. The results showed that the body weight gain was significantly...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01988

    authors: Li Y,Fu X,Ma X,Geng S,Jiang X,Huang Q,Hu C,Han X

    更新日期:2018-08-28 00:00:00

  • Long Chain Alcohols Produced by Trichoderma citrinoviride Have Phagodeterrent Activity against the Bird Cherry-Oat Aphid Rhopalosiphum padi.

    abstract::In this study we report the effects of fungal metabolites isolated from cultures of the fungus Trichoderma citrinoviride ITEM 4484 on the feeding preference of the aphid Rhopalosiphum padi, a major pest of cereal crops. Different phagodeterrent metabolites were purified by a combination of direct and reverse phase col...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00297

    authors: Ganassi S,Grazioso P,De Cristofaro A,Fiorentini F,Sabatini MA,Evidente A,Altomare C

    更新日期:2016-03-10 00:00:00

  • Aeolian Prokaryotic Communities of the Global Dust Belt Over the Red Sea.

    abstract::Aeolian prokaryotic communities (APC) are important components of bioaerosols that are transported freely or attached to dust particles suspended in the atmosphere. Terrestrial and marine ecosystems are known to release and receive significant prokaryote loads into and from the surrounded atmospheric air. However, com...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.538476

    authors: Aalismail NA,Díaz-Rúa R,Ngugi DK,Cusack M,Duarte CM

    更新日期:2020-11-12 00:00:00

  • Warming, but Not Acidification, Restructures Epibacterial Communities of the Baltic Macroalga Fucus vesiculosus With Seasonal Variability.

    abstract::Due to ocean acidification and global warming, surface seawater of the western Baltic Sea is expected to reach an average of ∼1100 μatm pCO2 and an increase of ∼5°C by the year 2100. In four consecutive experiments (spanning 10-11 weeks each) in all seasons within 1 year, the abiotic factors temperature (+5°C above in...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01471

    authors: Mensch B,Neulinger SC,Künzel S,Wahl M,Schmitz RA

    更新日期:2020-06-26 00:00:00

  • A High Throughput Assay for Screening Host Restriction Factors and Antivirals Targeting Influenza A Virus.

    abstract::Influenza A virus (IAV) is a human respiratory pathogen that causes seasonal epidemics and occasional global pandemics with devastating levels of morbidity and mortality. Currently approved treatments against influenza are losing effectiveness, as new viral strains are often refractory to conventional treatments. Thus...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00858

    authors: Wang L,Li W,Li S

    更新日期:2016-06-03 00:00:00