Abstract:
:The tomato leafminer, Tuta absoluta, now a major pest of tomato crops worldwide, is primarily controlled using chemical insecticides. Recently, high levels of resistance to the insecticide spinosad have been described in T. absoluta populations in Brazil. Selection of a resistant field-collected strain led to very high levels of resistance to spinosad and cross-resistance to spinetoram, but not to other insecticides that target the nicotinic acetylcholine receptor (nAChR). In this study the mechanisms underlying resistance to spinosad were investigated using toxicological, biochemical and molecular approaches. Inhibition of metabolic enzymes using synergists and biochemical assessment of detoxification enzyme activity provided little evidence of metabolic resistance in the selected strain. Cloning and sequencing of the nAChR α6 subunit from T. absoluta, the spinosad target-site, from susceptible and spinosad-resistant strains were done to investigate the role of a target-site mechanism in resistance. A single nucleotide change was identified in exon 9 of the α6 subunit of the resistant strain, resulting in the replacement of the glycine (G) residue at position 275 observed in susceptible T. absoluta strains with a glutamic acid (E). A high-throughput DNA-based diagnostic assay was developed and used to assess the prevalence of the G275E mutation in 17 field populations collected from different geographical regions of Brazil. The resistant allele was found at low frequency, and in the heterozygous form, in seven of these populations but at much higher frequency and in the homozygous form in a population collected in the Iraquara municipality. The frequency of the mutation was significantly correlated with the mortality of these populations in discriminating dose bioassays. In summary our results provide evidence that the G275E mutation is an important mechanism of resistance to spinosyns in T. absoluta, and may be used as a marker for resistance monitoring in field populations.
journal_name
Pestic Biochem Physioljournal_title
Pesticide biochemistry and physiologyauthors
Silva WM,Berger M,Bass C,Williamson M,Moura DM,Ribeiro LM,Siqueira HAdoi
10.1016/j.pestbp.2016.02.006subject
Has Abstractpub_date
2016-07-01 00:00:00pages
1-8eissn
0048-3575issn
1095-9939pii
S0048-3575(16)30013-Xjournal_volume
131pub_type
杂志文章abstract::JS399-19 (2-cyano-3-amino-3-phenylancryic acetate), a novel cyanoacrylate fungicide, has powerful inhibition against Fusarium species, especially to Fusarium graminearum. Treated with JS399-19, mycelium of F. graminearum was distorted and swelled. The embranchment increased. In order to investigate the effect of JS399...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2013.05.009
更新日期:2013-09-01 00:00:00
abstract::The development of plant protection product (PPPs)-resistant populations of plant pathogens, pests, and weeds, represents a major challenge that the crop protection sector is facing. Focusing on plant pathogenic fungi, the increased efflux of the active ingredients (a.i.) from the cytoplasm is highly correlated to ele...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2019.09.013
更新日期:2020-05-01 00:00:00
abstract::A series of novel aryloxyphenoxypropionate (APP) herbicides containing benzofuran moiety were designed, synthesized and tested for herbicidal activity. The bioassay results indicated that most of target compounds possessed moderate to good herbicidal activity against monocotyledonous weeds. Compounds 5a-5d and 6a-6d s...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2018.12.011
更新日期:2019-02-01 00:00:00
abstract::Unlike chemical pesticides, antiviral plants are biodegradable, replenishable and safe. In this study, 14 sesquiterpene compounds from Tithonia diversifolia were tested for their activities against Tobacco mosaic virus (TMV) using the half-leaf method. Tagitinin C (Ses-2) and 1β-methoxydiversifolin-3-0-methyl ether (S...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2017.05.009
更新日期:2017-08-01 00:00:00
abstract::Rhinella icterica is a poisonous toad whose toxic secretion has never been studied against entomotoxic potential. Sublethal doses of Rhinella icterica toxic secretion (RITS) were assayed in Nauphoeta cinerea cockroaches, in order to understand the physiological and behavioral parameters, over the insect central and pe...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2018.04.016
更新日期:2018-06-01 00:00:00
abstract::Mature and healthy male lesser bandicoot rats, Bandicota bengalensis (n = 40) were fed on bait (mixture of cracked wheat and powdered sugar in 98:2) containing different concentrations of triptolide (0, 0.15, 0.20 and 0.25% w/w) for 15 days in two-choice trials. Results revealed no significant effect of triptolide tre...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2014.09.012
更新日期:2014-11-01 00:00:00
abstract::Coffee leaf rust (CLR), caused by Hemileia vastatrix, is a major disease affecting coffee production worldwide. In this study, an in-depth analysis of the photosynthetic performance of coffee leaves challenged or not with H. vastatrix and sprayed with either epoxiconazole (EPO) or pyraclostrobin (PYR) was performed by...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2015.01.016
更新日期:2015-09-01 00:00:00
abstract::Combined application of plant essential oils (EOs) with known antimicrobial effects and silica nanocapsules with high loading capacity and protection capability of the EOs make them proper candidates for creating environmentally friendly fungicides. In this study, EOs of the Lemongrass (LGO) and Clove (CO) were used a...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2020.104696
更新日期:2020-11-01 00:00:00
abstract::Asia minor bluegrass (Polypogon fugax) is a common annual grass weed of winter crops distributed across China. We conducted a study on the resistance level and the mechanism of resistance to ACCase-inhibiting herbicides in a P. fugax population from China. Whole-plant dose-response experiments in greenhouse showed tha...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2013.11.001
更新日期:2014-01-01 00:00:00
abstract::The effects of a protease inhibitor protein from Xenorhabdus bovienii strain xbi1 (Xbpi-1) on biochemistry and physiology of pea aphid (Acyrthosiphon pisum) were studied, and the effects of Xbpi-1 on the activities of aphid aminopeptidase and the concentrations of carbohydrates, free amino acids and lipids in A. pisum...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2013.12.010
更新日期:2014-01-01 00:00:00
abstract::2-Allylphenol (2-AP) is an effective fungicide against a number of plant pathogens, which can be metabolized and bio-transformed to four chemical compounds by Rhizoctonia cerealis. To determine if its degradation affects antifungal activity, two major metabolites derived from 2-AP including 2-(2-hydroxypropyl) phenol ...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2016.06.006
更新日期:2017-01-01 00:00:00
abstract::Biotypes of Echinochloa crus-galli var. formosensis with resistance to cyhalofop-butyl, an acetyl-CoA carboxylase (ACCase) inhibitor, have been found in dry-seeded rice fields in Okayama, Japan. We collected two lines with suspected resistance (Ecf27 and Ecf108) from dry-seeded rice fields and investigated their sensi...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2015.02.007
更新日期:2015-03-01 00:00:00
abstract::Meta-diamides [3-benzamido-N-(4-(perfluoropropan-2-yl)phenyl)benzamides] are a distinct class of RDL GABA receptor noncompetitive antagonists showing high insecticidal activity against Spodoptera litura. The mode of action of the meta-diamides was demonstrated to be distinct from that of conventional noncompetitive an...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章,评审
doi:10.1016/j.pestbp.2014.09.010
更新日期:2015-06-01 00:00:00
abstract::The two-spotted spider mite Tetranychus urticae is a serious pest of many agricultural crops and ornamental plants. The sublethal effects of a new chemical, spinetoram, on T. urticae were investigated by treating adult females and eggs with LC10 and LC20 in the laboratory. The data were assessed based on age-stage, tw...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2016.02.002
更新日期:2016-09-01 00:00:00
abstract::The effects of s-metolachlor chronic exposure at concentrations of 1.1 μg/L (maximal real environmental concentration in the Czech Republic), 11 μg/L (environmental relevant concentration) and 110 μg/L on early life stages of marbled crayfish (Procambarus virginalis) was evaluated under laboratory conditions. All s-me...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2018.11.007
更新日期:2019-01-01 00:00:00
abstract::Although there have been many reports on the synergistic interactions among the major constituents of plant essential oils regarding insecticidal activity, their underlying mechanism of synergy is poorly understood. In our previous studies, we found each of the two most abundant constituents of rosemary (Rosmarinus of...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2017.01.012
更新日期:2017-08-01 00:00:00
abstract::Although insecticide resistance is a widespread problem for most insect pests, frequently the assessment of resistance occurs over a limited geographic range. Herein, we report the first widespread survey of insecticide resistance in the USA ever undertaken for the house fly, Musca domestica, a major pest in animal pr...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2013.10.006
更新日期:2013-11-01 00:00:00
abstract::This study proposes a biochemical and molecular model for the interaction between the Drosophila suzukii type 1 tyramine receptor (DsTAR1) and monoterpenes. A preliminary molecular and functional characterization of DsTAR1 cDNA revealed that a 1.8 kb long ORF codes for a 600 amino acid polypeptide featuring seven tran...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2020.02.015
更新日期:2020-05-01 00:00:00
abstract::1-Deoxynojirimycin (DNJ) is a natural d-glucose analogue from mulberry with promising physiological activity in vivo. Up to the present, the antidiabetic effects of DNJ on lowering blood sugar and accelerating lipid metabolism in mammals were broadly reported, but the specific character of DNJ against insects was vast...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2018.08.009
更新日期:2018-11-01 00:00:00
abstract::Control of Bemisia tabaci has depended primarily and heavily on insecticides, especially neonicotinoids. The novel sulfoximine insecticide sulfoxaflor exhibits high potency against a broad range of sap-feeding insect species, including those resistant to neonicotinoids. The resistance levels of Q-biotype B. tabaci fie...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2016.08.005
更新日期:2017-03-01 00:00:00
abstract::Although it is well known that Bacillus thuringiensis Cry toxins kill insect pest by disrupting midgut cells of susceptible larvae through their pore formation activity, it is not clear what intracellular events are triggered after pore formation on the cell membrane of the target cells. Here we analyzed the role of C...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2020.104728
更新日期:2021-01-01 00:00:00
abstract::Cantharidin (CTD) is a natural toxin with effective toxicity to lepidopteran pests. Nevertheless, little information is available on whether pests develop resistance to CTD. After being exposed to CTD (50 mg/L to 90 mg/L) or 10 generations, the resistance ratio of laboratory selected cantharidin-resistant Mythimna sep...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2020.104769
更新日期:2021-02-01 00:00:00
abstract::Due to the increasing use of chlorinated organic compounds, environmental pollution is a key issue in agricultural and industrial areas. In this study, biodegradation of chloroacetanilide herbicides, such as alachlor and metolachlor, by eight fungal strains of Trichoderma spp. originating from different microorganism ...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2019.11.018
更新日期:2020-02-01 00:00:00
abstract::Although phosphite (Phi)-based fertilizers are used in large quantities in agriculture, the use of Phi-based fungicides against soybean root rot caused by Phytophthora sojae are limited. While, their low toxicity are of high ecological and economic focus. Limited attention has been paid to Phi translocation efficiency...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2020.104757
更新日期:2021-02-01 00:00:00
abstract::Synergism and metabolic studies were conducted to identify the resistance mechanism against indoxacarb in two Choristoneura rosaceana (Harris) field populations compared to a susceptible population. The synergism study was carried out using diet incorporation bioassay for indoxacarb and the three synergists PBO, DEM, ...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2020.104636
更新日期:2020-09-01 00:00:00
abstract::The evolution of resistant weed populations in response to intensive herbicide selection pressure is a global issue. Resistance to post-emergence herbicides is widespread, whereas soil-applied pre-emergence herbicides can often remain effective. For example, in Australia pyroxasulfone is a new pre-emergence soil-appli...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2018.03.017
更新日期:2018-06-01 00:00:00
abstract::The European red mite Panonychus ulmi (Koch) is a major pest of apple trees worldwide and causes significant damage to apple orchards in Iran. Pyrethroid insecticides/acaricides, such as fenpropathrin and fenvalerate, are widely used to control P. ulmi, but their long-term use may lead to low efficacy. Earlier studies...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2019.03.008
更新日期:2019-06-01 00:00:00
abstract::Benzoylcyclohexanedione herbicides work by inhibiting 4-hydroxyphenylpyruvate dioxygenase which was the last new target site introduced for herbicides. In an attempt to find new 4-hydroxyphenylpyruvate dioxygenase inhibitors with high efficacy and selectivity, a novel benzoylcyclohexanedione compound SYP-9121 was synt...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2017.07.002
更新日期:2017-10-01 00:00:00
abstract::The objective of this study was to determine the structural requirements of organophosphorus insecticides (OPI) for reducing chicken embryo nicotinamide adenine dinucleotide (NAD(+)) content in OPI-induced teratogenesis and compare them with those needed for OPI inhibition of yolk sac membrane kynurenine formamidase (...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2015.10.015
更新日期:2016-05-01 00:00:00
abstract::Sanguinarine (Sang) is a natural alkaloid and distributed in several plants of Papaveraceae. The antitumor, antioxidant, antimicrobial and anti-inflammatory effects of Sang were extensively reported, but its speciality and mechanism against Lepidoptera insects were still unknown. In this study, detailed toxicological ...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2019.08.004
更新日期:2019-10-01 00:00:00