当前位置： SCI文献检索 > PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY期刊下所有文献 > Metabolic mechanisms of indoxacarb resistance in field populations of Choristoneura rosaceana (Harris) (Lepidoptera: Tortricidae).

Metabolic mechanisms of indoxacarb resistance in field populations of Choristoneura rosaceana (Harris) (Lepidoptera: Tortricidae).

Abstract：

:Synergism and metabolic studies were conducted to identify the resistance mechanism against indoxacarb in two Choristoneura rosaceana (Harris) field populations compared to a susceptible population. The synergism study was carried out using diet incorporation bioassay for indoxacarb and the three synergists PBO, DEM, and DEF. The metabolic study consists of indoxacarb in vitro reaction with fifth instar larvae 12,000 g midgut supernatant or with pre-inhibited (in vivo by the esterases inhibitor DEF) fifth instar larvae 12,000 g midgut supernatant at different incubation times. In both susceptible and cherry populations, only DEF significantly synergized indoxacarb with a synergism ratio (SR) of 6.5 and 22.6-fold respectively indicating an involvement of esterases in the both populations. In the apple population, all synergists PBO, DEM, and DEF significantly synergized indoxacarb with SR of 9.6, 7.7, and 285.6-fold respectively indicating a complex resistance case with the possible involvement of all three metabolic resistance mechanisms with the central role of esterase enzymes. In vitro, the indoxacarb (DPX-JW062) was very rapidly metabolized within 5 min into small molecules in the lower portion of the metabolic pathway when it reacted with the midgut supernatant of each population. None of the metabolites in the upper portion of the metabolic pathway were detected at any incubation time including the potent sodium channel blocker DCJW metabolite. The two field populations showed significantly higher rates of metabolism of DPX-JW062 compared to the susceptible population at five min of incubation and that may explain the presence of indoxacarb resistance. In the second part of the in vitro study, the bio-transformation of DPX-JW062 was remarkably decreased when it reacted with the pre-inhibited (by DEF) midgut supernatant of each population. Additionally, the degradation of metabolites in the upper portion of the metabolic pathway remarkably decreased, which resulted in accumulation of DCJW and MP819 metabolites. The accumulation of DCJW metabolite under the pre-inhibited midgut supernatants treatment provided a persuasive explanation of the synergistic impact of esterase inhibitor DEF on indoxacarb in C. rosaceana.

journal_name

Pestic Biochem Physiol

journal_title

Pesticide biochemistry and physiology

authors

Hafez AM,Mota-Sanchez D,Hollingworth RM,Vandervoort C,Wise JC

doi

10.1016/j.pestbp.2020.104636

subject

Has Abstract

pub_date

2020-09-01 00:00:00

pages

104636

eissn

0048-3575

issn

1095-9939

pii

S0048-3575(20)30131-0

journal_volume

168

pub_type

杂志文章

在线工具

Metabolic mechanisms of indoxacarb resistance in field populations of Choristoneura rosaceana (Harris) (Lepidoptera: Tortricidae).