Abstract：
:We have studied the mode of action of the insecticide spirotetramat in the nematode Caenorhabditis elegans. A combination of symptomology, forward genetics and genome editing show that spirotetramat acts on acetyl-CoA carboxylase (ACC) in C. elegans, as it does in insects. We found C. elegans embryos exposed to spirotetramat show a cell division defect which closely resembles the phenotype of loss-of-function mutations in the gene pod-2, which encodes ACC. We then identified two mutations in the carboxyl transferase domain of pod-2 (ACC) which confer resistance and were confirmed using CRISPR/Cas9. One of these mutations substitutes an invertebrate-specific amino acid with one ubiquitous in other taxa; this residue may, therefore, be a determinant of the selectivity of spirotetramat for invertebrates. Such a mutation may also be the target of selection for resistance in the field. Our study is a further demonstration of the utility of C. elegans in studying bioactive chemicals.
journal_name
Pestic Biochem Physioljournal_title
Pesticide biochemistry and physiologyauthors
Guest M,Kriek N,Flemming AJdoi
10.1016/j.pestbp.2020.104604subject
Has Abstractpub_date
2020-10-01 00:00:00pages
104604eissn
0048-3575issn
1095-9939pii
S0048-3575(20)30099-7journal_volume
169pub_type
杂志文章abstract：:Bentazon is a widely used herbicide that selectively removes broad-leaf weeds by competing with plastoquinone for the binding site in the D1 protein and interrupting the PET (photosynthetic electron transfer) chain. However, monocotyledonous plants, such as rice, show strong resistance to bentazon due to CYP81A6 induc...
journal_title：Pesticide biochemistry and physiology
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journal_title：Pesticide biochemistry and physiology
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journal_title：Pesticide biochemistry and physiology
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journal_title：Pesticide biochemistry and physiology
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journal_title：Pesticide biochemistry and physiology
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journal_title：Pesticide biochemistry and physiology
pub_type： 杂志文章,评审
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更新日期：2015-06-01 00:00:00
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journal_title：Pesticide biochemistry and physiology
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journal_title：Pesticide biochemistry and physiology
pub_type： 杂志文章
doi：10.1016/j.pestbp.2014.07.010
更新日期：2014-10-01 00:00:00
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journal_title：Pesticide biochemistry and physiology
pub_type： 杂志文章
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更新日期：2019-09-01 00:00:00
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journal_title：Pesticide biochemistry and physiology
pub_type： 杂志文章
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更新日期：2018-07-01 00:00:00
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journal_title：Pesticide biochemistry and physiology
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journal_title：Pesticide biochemistry and physiology
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journal_title：Pesticide biochemistry and physiology
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journal_title：Pesticide biochemistry and physiology
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journal_title：Pesticide biochemistry and physiology
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journal_title：Pesticide biochemistry and physiology
pub_type： 杂志文章
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更新日期：2018-02-01 00:00:00
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journal_title：Pesticide biochemistry and physiology
pub_type： 杂志文章
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更新日期：2018-09-01 00:00:00
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journal_title：Pesticide biochemistry and physiology
pub_type： 杂志文章
doi：10.1016/j.pestbp.2017.04.007
更新日期：2017-06-01 00:00:00
abstract：:Deltamethrin belongs to the class of synthetic pyrethroids, which are being widely used as insecticides in agricultural practices. Voltage-gated sodium channels (VGSCs) are the primary targets of these chemicals for toxicity to insects. Caenorhabditis elegans (C. elegans) does not have VGSCs but is susceptible to delt...
journal_title：Pesticide biochemistry and physiology
pub_type： 杂志文章
doi：10.1016/j.pestbp.2017.03.005
更新日期：2017-05-01 00:00:00
