Proteomic analysis of Fusarium graminearum treated by the fungicide JS399-19.

Abstract:

:JS399-19 (2-cyano-3-amino-3-phenylancryic acetate), a novel cyanoacrylate fungicide, has powerful inhibition against Fusarium species, especially to Fusarium graminearum. Treated with JS399-19, mycelium of F. graminearum was distorted and swelled. The embranchment increased. In order to investigate the effect of JS399-19 on protein expression of F. graminearum, total protein of F. graminearum cultured in normal condition and that treated with 0.5 μg/mL (EC90 value) JS399-19 were extracted respectively and proteomic analysis was performed using two-dimensional gel electrophoresis. The expression levels of 38 proteins varied quantitatively at least twofold. 33 proteins out of the 38 were successfully identified by MALDI-TOF-MS/MS and MASCOT. According to the classification of physiological functions from Conserved Domain Database analysis, 19, 5, 2, 3, 2 and 2 proteins were respectively associated with metabolism, regulation, motility, defense, signal transduction, and unknown function, which indicated that energy metabolism, the synthesis and transport of proteins and DNA of F. graminearum were inhibited by JS399-19 in different degrees. The expression levels of the genes were further confirmed by quantitative real-time PCR analyses. This study represents the first proteomic analysis of F. graminearum treated by JS399-19 and will provide some useful information to find the mode of action of the fungicide against F. graminearum.

journal_name

Pestic Biochem Physiol

authors

Hou Y,Zheng Z,Xu S,Chen C,Zhou M

doi

10.1016/j.pestbp.2013.05.009

subject

Has Abstract

pub_date

2013-09-01 00:00:00

pages

86-92

issue

1

eissn

0048-3575

issn

1095-9939

pii

S0048-3575(13)00093-X

journal_volume

107

pub_type

杂志文章
  • Brusatol isolated from Brucea javanica (L.) Merr. induces apoptotic death of insect cell lines.

    abstract::Brucea javanica (L.) Merr. is a medicine plant distributed widely throughout Asia where its bitter fruits have been used traditionally in medicine for treating various ailments and controlling some pests. In recent years, concerns over the potential impact of synthetic pesticides on human health and environment have n...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2013.04.007

    authors: Zhang L,Feng X,Ma D,Yang J,Jiang H,Zhang Y,He W

    更新日期:2013-09-01 00:00:00

  • Proteomics-based identification and analysis proteins associated with spirotetramat tolerance in Aphis gossypii Glover.

    abstract::Spirotetramat were now widely used for control insecticides resistant aphids since 2011 in China. In order to elucidate the possible resistance mechanism, a laboratory selected resistant strain (SR) of cotton aphid was established with a 578.93-fold and 14.91-fold resistance ratio to spirotetramat for adult aphids and...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2015.02.002

    authors: Xi J,Pan Y,Wei Z,Yang C,Gao X,Peng T,Bi R,Liu Y,Xin X,Shang Q

    更新日期:2015-03-01 00:00:00

  • Sublethal effects of spinetoram on the two-spotted spider mite, Tetranychus urticae (Acari: Tetranychidae).

    abstract::The two-spotted spider mite Tetranychus urticae is a serious pest of many agricultural crops and ornamental plants. The sublethal effects of a new chemical, spinetoram, on T. urticae were investigated by treating adult females and eggs with LC10 and LC20 in the laboratory. The data were assessed based on age-stage, tw...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2016.02.002

    authors: Wang L,Zhang Y,Xie W,Wu Q,Wang S

    更新日期:2016-09-01 00:00:00

  • Recent advances in tenuazonic acid as a potential herbicide.

    abstract::Tenuazonic acid (TeA), belonging to tetramic acids that are the largest family of natural products, is a mycotoxin produced by members of the genus Alternaria and other phytopathogenic fungi. TeA has many desirable bioactivities. In the past two decades, several studies have addressed its phytotoxic activity. Because ...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章,评审

    doi:10.1016/j.pestbp.2017.01.003

    authors: Chen S,Qiang S

    更新日期:2017-11-01 00:00:00

  • Lack of cross-resistance between neonicotinoids and sulfoxaflor in field strains of Q-biotype of whitefly, Bemisia tabaci, from eastern China.

    abstract::Control of Bemisia tabaci has depended primarily and heavily on insecticides, especially neonicotinoids. The novel sulfoximine insecticide sulfoxaflor exhibits high potency against a broad range of sap-feeding insect species, including those resistant to neonicotinoids. The resistance levels of Q-biotype B. tabaci fie...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2016.08.005

    authors: Wang W,Wang S,Han G,Du Y,Wang J

    更新日期:2017-03-01 00:00:00

  • Identification and functional analysis of a cytochrome P450 gene CYP9AQ2 involved in deltamethrin detoxification from Locusta migratoria.

    abstract::A 1578-bp cDNA of a cytochrome P450 gene (CYP9AQ2) was sequenced from the migratory locust, Locusta migratoria. It contains an open reading frame (ORF) of 1557 bp that encodes 519 amino acid residues. As compared with other known insect cytochrome P450 enzymes, the overall structure of its deduced protein is highly co...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2015.01.003

    authors: Guo Y,Zhang X,Wu H,Yu R,Zhang J,Zhu KY,Guo Y,Ma E

    更新日期:2015-07-01 00:00:00

  • Investigations of the constitutive overexpression of CYP6D1 in the permethrin resistantLPR strain of house fly (Musca domestica).

    abstract::House fly (Musca domestica) CYP6D1 is a cytochrome P450 involved in metabolism of xenobiotics. CYP6D1 is located on chromosome 1 and its expression is inducible in response to the prototypical P450 inducer phenobarbital (PB) in insecticide susceptible strains. Increased transcription of CYP6D1 confers resistance to pe...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2011.02.012

    authors: Lin GG,Scott JG

    更新日期:2011-06-01 00:00:00

  • Naturally potential antiviral agent polysaccharide from Dendrobium nobile Lindl.

    abstract::A polysaccharide DNPE6(11) was purified from Dendrobium nobile Lindl. (D. nobile Lindl.). Its structural characteristic, antiviral activity, and preliminary mechanism were studied. The structural characteristic analysis indicated that DNPE6(11) was a novel homogenous heteropolysaccharide from D. nobile Lindl. Bioactiv...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2020.104598

    authors: Li Z,Xiang J,Hu D,Song B

    更新日期:2020-07-01 00:00:00

  • A Pediococcus strain to rescue honeybees by decreasing Nosema ceranae- and pesticide-induced adverse effects.

    abstract::Honeybees ensure a key ecosystemic service by pollinating many agricultural crops and wild plants. However, since few decades, managed bee colonies have declined worldwide. This phenomenon is considered to be multifactorial, with a strong emphasis on both parasites and pesticides. Infection by the parasite Nosema cera...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2019.11.006

    authors: Peghaire E,Moné A,Delbac F,Debroas D,Chaucheyras-Durand F,El Alaoui H

    更新日期:2020-02-01 00:00:00

  • Pyrethrum-extract induced autophagy in insect cells: A new target?

    abstract::Pyrethrum extract (PY) is a natural insecticide that is extensively used across the world, and its insecticidal activity is attributed to the presence of six active esters known as pyrethrins. PY targets the nervous systems of insects by delaying the closure of voltage-gated sodium ion channels in the nerve cells. How...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2016.09.003

    authors: Xu Z,Lu M,Yang M,Xu W,Gao J,Zhang Y,Yang Y,Tao L

    更新日期:2017-04-01 00:00:00

  • Sanguinarine caused larval lethality and growth inhibition by suppressing energy metabolism in silkworms, Bombyx mori.

    abstract::Sanguinarine (Sang) is a natural alkaloid and distributed in several plants of Papaveraceae. The antitumor, antioxidant, antimicrobial and anti-inflammatory effects of Sang were extensively reported, but its speciality and mechanism against Lepidoptera insects were still unknown. In this study, detailed toxicological ...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2019.08.004

    authors: Li P,Hu JW,Wen CW,Hang Y,Zhou ZH,Xie M,Lv JC,Wang CM,Huang YH,Xu JP,Deng MJ

    更新日期:2019-10-01 00:00:00

  • IRAC: Mode of action classification and insecticide resistance management.

    abstract::Insecticide resistance is a long standing and expanding problem for pest arthropod control. Effective insecticide resistance management (IRM) is essential if the utility of current and future insecticides is to be preserved. Established in 1984, the Insecticide Resistance Action Committee (IRAC) is an international as...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2014.11.014

    authors: Sparks TC,Nauen R

    更新日期:2015-06-01 00:00:00

  • Cellular destruction, phytohormones and growth modulating enzymes production by Bacillus subtilis strain BC8 impacted by fungicides.

    abstract::In vitro experiments were performed to ascertain the impact of kitazin, hexaconazole, metalaxyl and carbendazim on growth behaviour, enzymatic profile, ultrastructure, cell permeability and bioactive molecules of phosphate-solubilizing bacterium. Strain BC8 isolated from Brassica oleracea rhizosphere was characterized...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2018.05.001

    authors: Shahid M,Khan MS

    更新日期:2018-07-01 00:00:00

  • Target-site and non-target-site-based resistance to tribenuron-methyl in multiply-resistant Myosoton aquaticum L.

    abstract::Myosoton aquaticum L., a widespread and competitive winter weed of wheat in China, has evolved resistance to many classes of herbicides. In one M. aquaticum population (AH03), collected from Anhui Province, where tribenuron-methyl and florasulam had been used to control this weed resistance to both herbicides had evol...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2018.12.004

    authors: Bai S,Zhang F,Li Z,Wang H,Wang Q,Wang J,Liu W,Bai L

    更新日期:2019-03-01 00:00:00

  • Histomorphological and biochemical changes induced by triptolide treatment in male lesser bandicoot rat, Bandicota bengalensis.

    abstract::Mature and healthy male lesser bandicoot rats, Bandicota bengalensis (n = 40) were fed on bait (mixture of cracked wheat and powdered sugar in 98:2) containing different concentrations of triptolide (0, 0.15, 0.20 and 0.25% w/w) for 15 days in two-choice trials. Results revealed no significant effect of triptolide tre...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2014.09.012

    authors: Dhar P,Singla N

    更新日期:2014-11-01 00:00:00

  • Inhibition of acetylcholinesterases of the pinewood nematode, Bursaphelenchus xylophilus, by phytochemicals from plant essential oils.

    abstract::To understand the nematicidal mode of action of phytochemicals derived from plant essential oils against the pinewood nematode (Bursaphelenchus xylophilus), we evaluated 97 compounds (49 monoterpenes, 17 phenylpropenes, 16 sesquiterpenes, and 15 sulfides) for their inhibitory effects on B. xylophilus acetylcholinester...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2012.11.007

    authors: Kang JS,Kim E,Lee SH,Park IK

    更新日期:2013-01-01 00:00:00

  • Synthesis and biological activity of benzoylcyclohexanedione herbicide SYP-9121.

    abstract::Benzoylcyclohexanedione herbicides work by inhibiting 4-hydroxyphenylpyruvate dioxygenase which was the last new target site introduced for herbicides. In an attempt to find new 4-hydroxyphenylpyruvate dioxygenase inhibitors with high efficacy and selectivity, a novel benzoylcyclohexanedione compound SYP-9121 was synt...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2017.07.002

    authors: Li H,Liu C,Guan A,Wang Z,Ma H,Cui D

    更新日期:2017-10-01 00:00:00

  • Oxidative stress induced by chlorine dioxide as an insecticidal factor to the Indian meal moth, Plodia interpunctella.

    abstract::A novel fumigant, chlorine dioxide (ClO2) is a commercial bleaching and disinfection agent. Recent study indicates its insecticidal activity. However, its mode of action to kill insects is yet to be understood. This study set up a hypothesis that an oxidative stress induced by ClO2 is a main factor to kill insects. Th...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2015.04.003

    authors: Kumar S,Park J,Kim E,Na J,Chun YS,Kwon H,Kim W,Kim Y

    更新日期:2015-10-01 00:00:00

  • Biogenetic cantharidin is a promising leading compound to manage insecticide resistance of Mythimna separata (Lepidoptera: Noctuidae).

    abstract::Cantharidin (CTD) is a natural toxin with effective toxicity to lepidopteran pests. Nevertheless, little information is available on whether pests develop resistance to CTD. After being exposed to CTD (50 mg/L to 90 mg/L) or 10 generations, the resistance ratio of laboratory selected cantharidin-resistant Mythimna sep...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2020.104769

    authors: Li Y,Sun H,Yasoob H,Tian Z,Li Y,Li R,Zheng S,Liu J,Zhang Y

    更新日期:2021-02-01 00:00:00

  • Limited variations in susceptibility to an insecticidal double-stranded RNA (dsvATPaseE) among a laboratory strain and seven genetically differentiated field populations of Tribolium castaneum.

    abstract::There has been a considerable growth in interest to use RNA interference (RNAi) as a novel insect pest management strategy in the past 10 years. However, there has been virtually no information on insect population variations in response to double-stranded RNA (dsRNA) molecules. The objective of this study was to gene...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2018.06.005

    authors: Wang H,Zhang J,Zhao S,Zhu KY,Wu Y

    更新日期:2018-07-01 00:00:00

  • Bacteria-mediated modification of insecticide toxicity in the yellow fever mosquito, Aedes aegypti.

    abstract::The incidence of mosquito-borne disease poses a significant threat to human and animal health throughout the world, with effective chemical control interventions limited by widespread insecticide resistance. Recent evidence suggests that gut bacteria of mosquitoes, known to be essential in nutritional homeostasis and ...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2019.07.016

    authors: Scates SS,O'Neal ST,Anderson TD

    更新日期:2019-11-01 00:00:00

  • Metabolic mechanisms of indoxacarb resistance in field populations of Choristoneura rosaceana (Harris) (Lepidoptera: Tortricidae).

    abstract::Synergism and metabolic studies were conducted to identify the resistance mechanism against indoxacarb in two Choristoneura rosaceana (Harris) field populations compared to a susceptible population. The synergism study was carried out using diet incorporation bioassay for indoxacarb and the three synergists PBO, DEM, ...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2020.104636

    authors: Hafez AM,Mota-Sanchez D,Hollingworth RM,Vandervoort C,Wise JC

    更新日期:2020-09-01 00:00:00

  • Isoxadifen safening mechanism in sweet corn genotypes with differential response to P450-metabolized herbicides.

    abstract::Three sweet corn genotypes, two inbred lines (IBER001 and IBER002) and their hybrid (ER00X), differ in their phenotypic responses to several P450-metabolized herbicides, used in sweet corn, namely, foramsulfuron, iodosulfuron, rimsulfuron and tembotrione. Foramsulfuron is a sulfonylurea herbicide commonly formulated w...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2017.02.002

    authors: Paporisch A,Rubin B

    更新日期:2017-05-01 00:00:00

  • Phosphite translocation in soybean and mechanisms of Phytophthora sojae inhibition.

    abstract::Although phosphite (Phi)-based fertilizers are used in large quantities in agriculture, the use of Phi-based fungicides against soybean root rot caused by Phytophthora sojae are limited. While, their low toxicity are of high ecological and economic focus. Limited attention has been paid to Phi translocation efficiency...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2020.104757

    authors: Guo M,Li B,Xiang Q,Wang R,Liu P,Chen Q

    更新日期:2021-02-01 00:00:00

  • Responses in reproductive organs, steroid hormones and CYP450 enzymes in female Mongolian gerbil (Meriones unguiculatus) over time after quinestrol treatment.

    abstract::The aim of this study was to assess the effects and reversibility of the synthetic estrogen compound, quinestrol, on the reproductive organs, steroid hormones, and drug-metabolizing enzymes CYP3A4 and CYP1A2 in liver and kidney over time after two quinestrol treatments in female Mongolian gerbils (Meriones unguiculatu...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2017.08.008

    authors: Su QQ,Chen Y,Qin J,Wang TL,Wang DH,Liu QS

    更新日期:2017-11-01 00:00:00

  • Overexpression of multiple cytochrome P450 genes associated with sulfoxaflor resistance in Aphis gossypii Glover.

    abstract::Sulfoxaflor is the first commercially available sulfoximine insecticide, which exhibits highly efficacy against many sap-feeding insect pests and has been applied as an alternative insecticide against cotton aphid in China. This study was conducted to investigate the risk of resistance development, the cross-resistanc...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2019.03.021

    authors: Ma K,Tang Q,Zhang B,Liang P,Wang B,Gao X

    更新日期:2019-06-01 00:00:00

  • Single-stranded DNA fragments of insect-specific nuclear polyhedrosis virus act as selective DNA insecticides for gypsy moth control.

    abstract::This paper focuses on the DNA insecticides as a novel preparation against gypsy moth (Lymantria dispar) based on DNA fragments of the anti-apoptotic gene of its nuclear polyhedrosis virus. It was found that the external application of a solution with two single-stranded DNA fragments from BIR and RING domains of LdMNP...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2014.05.005

    authors: Oberemok VV,Skorokhod OA

    更新日期:2014-07-01 00:00:00

  • Differential metabolism of neonicotinoids by brown planthopper, Nilaparvata lugens, CYP6ER1 variants.

    abstract::Imidacloprid is very effective in controlling Nilaparvata lugens Stål, which severely damages rice plants. Following heavy imidacloprid use, imidacloprid-resistant N. lugens, which showed cross-resistance to other neonicotinoids, appeared. We used the baculovirus/Sf9 expression system to express CYP6ER1 variants carry...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2020.02.004

    authors: Hamada A,Stam L,Nakao T,Kawashima M,Banba S

    更新日期:2020-05-01 00:00:00

  • The yeast (Saccharomyces cerevisiae) YCF1 vacuole transporter: Evidence on its implication into the yeast resistance to flusilazole as revealed by GC/EI/MS metabolomics.

    abstract::The development of plant protection product (PPPs)-resistant populations of plant pathogens, pests, and weeds, represents a major challenge that the crop protection sector is facing. Focusing on plant pathogenic fungi, the increased efflux of the active ingredients (a.i.) from the cytoplasm is highly correlated to ele...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2019.09.013

    authors: Karamanou DA,Aliferis KA

    更新日期:2020-05-01 00:00:00

  • RNA-Seq transcriptome analysis to identify candidate genes involved in non-target site-based mesosulfuron-methyl resistance in Beckmannia syzigachne.

    abstract::American sloughgrass (Beckmannia syzigachne Steud.) has become a dominant weed in fields with rice-wheat rotation. Moreover, herbicide resistance has rendered weed control difficult. We identified a biotype showing resistance to ALS inhibitor mesosulfuron-methyl with a resistant index 3.3, but without any ALS mutation...

    journal_title:Pesticide biochemistry and physiology

    pub_type: 杂志文章

    doi:10.1016/j.pestbp.2020.104738

    authors: Wang J,Chen J,Li X,Cui H

    更新日期:2021-01-01 00:00:00