Abstract:
:Autophagy has been shown to be involved in the pathophysiology of developmental seizure-induced brain damage. The present study aimed to examine whether E-64d, an autophagy inhibitor, was able to facilitate developmental seizure-induced hippocampal mossy fiber sprouting, in particular sprouting-associated zinc transporter signals. Recurrent seizures were induced by penicillin every other day in Sprague-Dawley rats from postnatal day 21 (P21). Rats were randomly assigned into the control group (CONT), recurrent seizure group (RS) and the seizure plus E-64d group (E64D). The expression levels of beclin-1 and B-cell lymphoma 2 were analyzed at 1.5, 3, 6 and 24 h after the last seizures using western blot analysis. At P51, mossy fiber sprouting and the mRNA expression levels of zinc transporter 2 (ZnT-2), ZnT-4, ZnT-5, ZnT-6, ZnT-7, divalent cation transporter 1, Zrt-Irt-like protein 6 (ZIP-6), ZIP-7, cathepsin D and cathepsin L in the rat hippocampus were assessed using Timm staining and reverse transcription-quantitative polymerase chain reaction analysis, respectively. Reduced hippocampal mossy fiber sprouting were detected in the E-64d-treated rats compared with the non-treated control. In parallel with these observations, there was a marked reduction in the mRNA expression levels of ZnT-4 at P51 in the E-64d-treated rat hippocampus compared with the non-treated seizure group. Linear correlation analysis showed significant inter-relationship among ZIP-7, ZnT-4, ZnT-5, ZnT-7, cathepsin D and cathepsin L. These results indicate that the ZnT-4/ZIP-7/cathepsin signaling pathway serves a crucial function in the neuroprotective effects of E-64d. Thus, E-64d may offer a novel strategy for the development of therapeutic interventions for developmental seizure-induced brain damage.
journal_name
Exp Ther Medjournal_title
Experimental and therapeutic medicineauthors
Ni H,Zhang LL,Ren SY,Sun BLdoi
10.3892/etm.2016.3276subject
Has Abstractpub_date
2016-07-01 00:00:00pages
208-214issue
1eissn
1792-0981issn
1792-1015pii
ETM-0-0-3276journal_volume
12pub_type
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journal_title:Experimental and therapeutic medicine
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journal_title:Experimental and therapeutic medicine
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doi:10.3892/etm.2017.4137
更新日期:2017-04-01 00:00:00
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2014.2152
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journal_title:Experimental and therapeutic medicine
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journal_title:Experimental and therapeutic medicine
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doi:10.3892/etm.2016.3543
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
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更新日期:2015-03-01 00:00:00
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm_00000082
更新日期:2010-05-01 00:00:00
abstract::MicroRNA (miR)-199b-5p has been reported to have a critical role in various types of malignancy. However, the exact function miR-199b-5p in renal cancer remains to be fully elucidated. The present study aimed to detect the expression levels of miR-199b-5p in renal cell carcinoma (RCC) tissues and RCC cell lines, and i...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2018.6151
更新日期:2018-07-01 00:00:00
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更新日期:2019-09-01 00:00:00
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journal_title:Experimental and therapeutic medicine
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pub_type: 杂志文章,评审
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2013.1188
更新日期:2013-09-01 00:00:00
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
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journal_title:Experimental and therapeutic medicine
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更新日期:2018-10-01 00:00:00
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章,已发布勘误
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更新日期:2019-04-01 00:00:00
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journal_title:Experimental and therapeutic medicine
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更新日期:2014-01-01 00:00:00
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
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