Engineering cell fate: Spotlight on cell-activation and signaling-directed lineage conversion.

Abstract:

:Direct reprogramming of specialized cells into other cell types has revolutionized the fields of stem cell, differentiation, and regenerative medicine. Direct reprogramming technology can convert various differentiated cell types to other fates by the forced expression of lineage-specific transcription factors. In addition to this approach, transdifferentiation can be induced in somatic cells by a method named cell-activation and signaling-directed (CASD) lineage conversion, which uses pluripotency reprogramming factors in combination with specific differentiation signals. This approach is capable of generating tissue-specific progenitors in addition to functional mature cells through a challenging transitory (pluripotent or non-pluripotent) state. Interestingly, the CASD lineage conversion has been accomplished by using small molecules and growth factors in a chemical-only paradigm. This approach will have a substantial positive impact on the field and bring reprogramming technology into the translational pipeline. From the regenerative medicine perspective, in future, new therapies might be designed based on the patient's own cells that are directly reprogrammed into the desired cell types in vitro and in situ. This review provides an overview of direct reprogramming to different lineages and describes what is known about the cell-activation and signaling-directed transdifferentiation.

journal_name

Tissue Cell

journal_title

Tissue & cell

authors

Ebrahimi B

doi

10.1016/j.tice.2016.07.005

subject

Has Abstract

pub_date

2016-10-01 00:00:00

pages

475-87

issue

5

eissn

0040-8166

issn

1532-3072

pii

S0040-8166(16)30086-6

journal_volume

48

pub_type

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