Abstract:
:Standard procedures for isolating nucleic acids from specialized tissues such as the mucus-containing tissues found in many marine organisms are, in many cases, not effective, resulting in isolates contaminated with polysaccharides that encumber subsequent analysis. A method is described for isolating nucleic acids from the sea anemone Condylactis gigantea (Cnidaria: Anthozoa) using the compound hexadecyltrimethyl ammonium bromide (CTAB). This substance has historically been effective in producing digestible chromosomal DNA from a variety of polysaccharide-enriched sources. In the presence of CTAB, DNA can be isolated and extracted from Condylactis gigantea and is suitable for digestion with restriction endonucleases. With a minor modification, RNA can also be extracted and used to obtain mRNA. The technique is useful for Cnidarian tissues and may be appropriate for a variety of other marine invertebrates and algae.
journal_name
Tissue Celljournal_title
Tissue & cellauthors
Dellacorte Cdoi
10.1016/0040-8166(94)90013-2subject
Has Abstractpub_date
1994-08-01 00:00:00pages
613-9issue
4eissn
0040-8166issn
1532-3072pii
0040-8166(94)90013-2journal_volume
26pub_type
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