The Effects of Melatonin on Ethylene Glycol-induced Nephrolithiasis: Role on Osteopontin mRNA Gene Expression.

Abstract:

OBJECTIVE:To evaluate the protective effects of melatonin (Mel) on an ethylene glycol (EG)-induced nephrolithiasis model in rats. MATERIALS AND METHODS:Thirty-two Wistar albino rats were divided into 4 groups: control, EG, prevention Mel (Mel + EG + Mel), and therapeutic Mel (EG + Mel). EG (0.75%) was added to drinking water to create nephrolithiasis model. The EG group received EG and the Mel + EG + Mel group received both EG and Mel for 8 weeks. In the EG + Mel group, EG is given for 8 weeks and Mel is given for the last 4 weeks of the experiment. At the end of experimental period, urine, blood samples, and tissues were collected. RESULTS:In 24-hour urine samples, calcium, citrate, and creatinine levels were decreased and oxalate levels were increased in the EG group, whereas Mel prevention and Mel treatment reversed these parameters back to control levels. Malondialdehyde, glutathione activities, myeloperoxidase, superoxide dismutase levels, and caspase-3 activity showed improvements in the Mel-treated groups when compared with the EG group. 8-Hydroxydeoxyguanosine, matrix metalloproteinase 9 levels, N-acetyl-β-glucosaminidase activity, and osteopontin mRNA expression were elevated in the EG group and decreased back to control levels in the Mel + EG + Mel and EG + Mel groups. Histological examination showed improvement in the Mel-treated groups when compared with the EG group. CONCLUSION:Mel can prevent crystalluria and kidney damage due to crystal formation and aggregation. It can be considered as a potential prophylactic and protective agent in high-risk patients with urinary stone formation or recurrence if supported by further clinical studies.

journal_name

Urology

journal_title

Urology

authors

Sener TE,Sener G,Cevik O,Eker P,Cetinel S,Traxer O,Tanidir Y,Akbal C

doi

10.1016/j.urology.2016.09.032

subject

Has Abstract

pub_date

2017-01-01 00:00:00

pages

287.e9-287.e15

eissn

0090-4295

issn

1527-9995

pii

S0090-4295(16)30651-3

journal_volume

99

pub_type

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