Abstract：
OBJECTIVE:To characterize human bladder smooth muscle cell reactions to disturbed oxygen tension. Clinical studies have shown a close correlation between bladder ischemia and lower urinary tract symptoms in elderly patients. MATERIALS AND METHODS:Confluent cultured human bladder smooth muscle cells were incubated under normoxia, continuous hypoxia, and oxidative stress (hypoxia/reoxygenation) conditions using a computerized oxycycler system. After 48 hours, cell samples were collected and processed for fluorometric assessment of oxidative injury, enzyme immunoassay of antioxidant capacity, and transmission electron microscopy. RESULTS:Lipid peroxidation was found in cell hypoxia and oxidative stress, whereas protein oxidation was evident in oxidative stress only. Cell antioxidant capacity decreased in oxidative stress but remained unchanged in hypoxia. Oxidative products were present in cell oxidative stress only, whereas nitrosative products increased in both hypoxia and oxidative stress conditions. Forty-eight hours of hypoxia and oxidative stress had no effect on cell senescence. Thickened deformed cell membrane, swollen mitochondria, and enlarged endoplasmic reticulum (ER) were found in cell hypoxia. Partially lost cell membrane with increased caveolae, swollen mitochondria with degraded cristae, splintered ER, and increased lysosomes were evident in cell oxidative stress. CONCLUSION:Human bladder smooth muscle cells are highly reactive to nonconforming oxygen tension. Reactions to hypoxia are consistent with cell survival signaling to cope with lack of oxygen. Changes in oxidative stress indicate extensive damage and deterioration of the subcellular elements. Hypoxic and oxidative damage may be an important mechanism of smooth muscle degeneration in bladder conditions with disturbed oxygen tension.
journal_name
Urologyjournal_title
Urologyauthors
Azadzoi KM,Yalla SV,Siroky MBdoi
10.1016/j.urology.2011.06.034subject
Has Abstractpub_date
2011-10-01 00:00:00pages
967.e9-15issue
4eissn
0090-4295issn
1527-9995pii
S0090-4295(11)00689-3journal_volume
78pub_type
杂志文章相关文献
UROLOGY文献大全abstract：:In many countries of the world, prostate cancer is the second most common form of cancer in men, and in the United States it is now in first rank. It is an important public health problem, with > 0.25 million new cases diagnosed worldwide in the year 1985. Whereas earlier large increases in the incidence of prostate c...
journal_title：Urology
pub_type： 杂志文章,评审
doi：10.1016/s0090-4295(99)80250-7
更新日期：1995-09-01 00:00:00
abstract：OBJECTIVE:To determine feasibility of an electromagnetic (EM) guidance system (Auris Health, Redwood City, CA) in obtaining percutaneous renal access among urologists and trainees of different experience levels. EM-guidance is appealing for access as it allows real time, 3-dimensional targeting without radiation. Few s...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2019.08.060
更新日期：2020-02-01 00:00:00
abstract：:A patient with polycystic disease was admitted with acute abdominal pain. This was caused by a rupture of one of his right renal cysts into the collecting system, with extravasation into the subcapsular space. With conservative therapy, the patient returned to his status prior to this episode. The extravasation was ab...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/0090-4295(76)90563-x
更新日期：1976-01-01 00:00:00
abstract：:We report a case of postoperative pediatric priapism in a boy without a hematologic disorder after a propofol-based general anesthetic. Our case underscores the importance of the early diagnosis and treatment to lessen the risk of complications. Given the wide use of propofol for sedation and anesthesia, clinicians sh...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2008.12.066
更新日期：2009-07-01 00:00:00
abstract：OBJECTIVE:Scrotal hypospadias still presents a challenge for reconstructive urologists. We present a novel and 1-stage technique of using a specially shaped buccal mucosa graft for simultaneous ventral tunica grafting and urethroplasty for severe hypospadias repair. METHODS:A 14-month old boy underwent repair of scrot...
journal_title：Urology
pub_type：
doi：10.1016/j.urology.2019.12.016
更新日期：2020-03-01 00:00:00
abstract：:Infants born with imperforate anus have a high incidence of genitourinary anomalies. Vesicoureteral reflux was the most common abnormality in this series. Since these patients often have bacteriuria, this is an important finding. An intravenous pyelogram and voiding cystourethrogram should be part of the evaluation of...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/0090-4295(79)90380-7
更新日期：1979-06-01 00:00:00
abstract：OBJECTIVES:Hormone-independent and cytotoxic drug-resistant tumor growth in osteoblastic metastases defines poor survival in patients with advanced prostate cancer. Therefore, we analyzed the ability of human osteoblast-like cells (MG-63 cells) and MG-63 conditioned media (MG-63 CM) to protect PC-3 human prostate cance...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/s0090-4295(98)00182-4
更新日期：1998-08-01 00:00:00
abstract：OBJECTIVE:To assess (1) plasma levels of antidiuretic hormone (ADH) and brain natriuretic peptide (BNP) and urinary levels of electrolytes in children with sleep disordered breathing (SDB), with or without nocturnal enuresis (NE), and (2) the effect of adenotonsillectomy (T&A) on urinary electrolytes and the secretion ...
journal_title：Urology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.urology.2015.04.011
更新日期：2015-07-01 00:00:00
abstract：OBJECTIVES:To describe a retrospective review of a single-institution, single-surgeon (M.G.) experience with 44 simplified Indiana pouch with multiple teniamyotomies without detubularization and reconfiguration. METHODS:From April 1999 to May 2003, 44 patients underwent radical cystectomy and continent urinary diversi...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2005.07.054
更新日期：2006-01-01 00:00:00
abstract：:We investigated the effect of two diets containing equal amounts of the carcinogen FANFT on the incidence and induction time of murine bladder carcinoma in mice. At each time interval, the tumor incidence in the mice ingesting a purified, high-calorie diet, was equal or greater than the tumor incidence in the animals ...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/0090-4295(81)90297-1
更新日期：1981-11-01 00:00:00
abstract：:From 1962 to 1978, 56 patients underwent transrenal endarterectomy as surgical treatment for renovascular hypertension. Over-all results were 39 per cent cured, 36 per cent improved, and 25 per cent failed. Criteria for selecting patients with atherosclerotic renal artery disease for surgical treatment are reviewed. T...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/0090-4295(79)90181-x
更新日期：1979-11-01 00:00:00
abstract：OBJECTIVES:To assess subjective and objective outcomes in incontinent patients following "out-in" TOT, at >10-year follow-up; to evaluate effect on quality of life and other urinary symptoms, late adverse events and predictive factors for failure. METHODS:This single-centre prospective study evaluated women with "comp...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2019.03.003
更新日期：2019-07-01 00:00:00
abstract：OBJECTIVES:There is little objective information on what is designated as being the prostate capsule. We have quantified objectively with computerized image analysis the percentage of smooth muscle fibers (SMFs) surrounding the surgical specimens of radical prostatectomies. METHODS:Ten radical prostatectomy specimens ...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/s0090-4295(99)80190-3
更新日期：1995-08-01 00:00:00
abstract：:Prostate cancer is dependent on androgen stimulation mediated by the androgen receptor (AR), a member of the steroid hormone receptor family of ligand-dependent nuclear receptors. Most patients respond to standard androgen ablation therapies, but virtually all patients eventually relapse with disease that has been ter...
journal_title：Urology
pub_type： 杂志文章,评审
doi：10.1016/s0090-4295(02)01593-5
更新日期：2002-09-01 00:00:00
abstract：:Although unusual, placement of a Foley catheter into the renal pelvis or ureter has been reported. Proposed predisposing factors for errant placement include pre-existing hydronephrosis or congenital ureteral orifice enlargement. We report a case where aberrant placement of a Foley catheter into the renal pelvis of a ...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2015.06.029
更新日期：2015-09-01 00:00:00
abstract：:We report on a twenty-four-year-old woman with extraosseous Ewing sarcoma in the right retroperitoneum. The tumor blocked the common iliac vein, and because of an intervening pregnancy, the blood flow was shunted through the uterus and left ovarian vein back into the vena cava. She died within nine months despite radi...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/0090-4295(81)90279-x
更新日期：1981-04-01 00:00:00
abstract：:The American Medical Systems (AMS) malleable penile prosthesis was implanted in 57 patients from January, 1983 to 1985. Fifty-one per cent of the procedures were performed under local anesthesia, and 28 per cent in ambulatory (day) surgery. The prosthesis was evaluated in terms of cosmetic result, patient satisfaction...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/0090-4295(86)90147-0
更新日期：1986-12-01 00:00:00
abstract：OBJECTIVES:In 2001, we reported a novel technique for pediatric circumcision that has proved to be fast, reliable, and safe during the past 4 years, with good cosmetic results. Inspired by our success with this technique, we have created a similar device to be used for adults. METHODS:We conducted a prospective, rando...
journal_title：Urology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.urology.2010.02.021
更新日期：2010-10-01 00:00:00
abstract：OBJECTIVE:To determine the long-term impact of extravasation at initial post-urethroplasty urethrogram, we present a novel classification system and report the association of leak severity with outcome. MATERIALS AND METHODS:A total of 91 patients underwent buccal graft urethroplasties of the bulbar urethra from 2007 ...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2017.02.015
更新日期：2017-06-01 00:00:00
abstract：:Cytologic differences in the urine of 6 patients with transitional cell carcinoma in situ (CIS) of the urinary bladder with and without prostatic duct involvement are described. In the former group, there was an admixture of CIS cells along with many bizarre malignant cells which increased with time and showed maligna...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/0090-4295(76)90203-x
更新日期：1976-05-01 00:00:00
abstract：OBJECTIVE:To determine the prevalence of penile cancer in patients with adult acquired buried penis (AABP). Penile cancer is a rare but aggressive cancer. Several case reports have recently been published that indicate that AABP may increase the risk of penile cancer. MATERIALS AND METHODS:A retrospective review was c...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2019.07.019
更新日期：2019-11-01 00:00:00
abstract：OBJECTIVES:To compare, retrospectively, the results of laparoscopic partial nephrectomy (LPN) to open partial nephrectomy (OPN) using a tumor size-matched cohort of patients. Limited data are available comparing LPN to OPN in the treatment of small renal tumors. METHODS:Between September 2000 and September 2003, 27 LP...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2004.04.028
更新日期：2004-09-01 00:00:00
abstract：:Congenital pelvic arteriovenous malformations are rare entities, especially in males. Presenting symptoms, if any at all, are commonly a mass, thrill, bruit, or pain. Treatment options include surgical extirpation, embolization, or a combination of both. This case provides support for the last option in a patient pres...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/s0090-4295(98)00352-5
更新日期：1999-02-01 00:00:00
abstract：:Twenty-five patients with renal cell carcinoma were treated with a lipophilic macromolecular drug, poly(stylene-co-maleic acid)-conjugated neocarzinostatin (SMANCS) dissolved in lipid contrast medium (Lipiodol). The drug was injected by catheterizing the renal artery and another feeding artery in 24 patients, and in t...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/0090-4295(91)80308-t
更新日期：1991-03-01 00:00:00
abstract：OBJECTIVE:Sacrocolpopexy is considered the gold standard for the treatment of pelvic organ prolapse (POP). This procedure was the first technique used to treat POP with robotic assistance and has gone a long way since the open procedure was introduced. Originally, 5 trocars were inserted and there remained issues in te...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2016.11.023
更新日期：2017-05-01 00:00:00
abstract：:To determine whether serum beta 2 microglobulin could be useful as a tumor marker in patients with testicular cancers, sera stored in our serum bank from 23 patients were assayed by radioimmunoassay for beta 2 microglobulin levels. Sera were selected from 12 preorchiectomy patients, 8 patients who had undergone orchie...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/0090-4295(80)90614-7
更新日期：1980-11-01 00:00:00
abstract：INTRODUCTION:To describe the operative details and results of a modified posterior sagittal transanorectal approach for the reconstruction of urogenital sinus (UGS) anomalies. TECHNICAL CONSIDERATIONS:Six children with UGS anomalies underwent surgery using this technique. In a prone jack-knife position, a midline inci...
journal_title：Urology
pub_type： 临床试验,杂志文章
doi：10.1016/j.urology.2007.04.017
更新日期：2007-09-01 00:00:00
abstract：:A case of a leiomyosarcoma developing in a penile prepuce of a young patient is presented with ultrastructural studies. The firm, well-circumscribed nodule was present for several years. Histologically and ultrastructurally the excised nodule showed features of a leiomyosarcoma. The incidence and morphology of this ra...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/0090-4295(86)90345-6
更新日期：1986-06-01 00:00:00
abstract：OBJECTIVE:To evaluate the impact of extended warm ischemia on incidence of acute kidney injury (AKI) and ultimate functional recovery after partial nephrectomy (PN), incorporating rigorous control for loss of parenchymal mass, and embedded within comparison to cohorts of patients managed with hypothermia or limited war...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2015.08.034
更新日期：2016-01-01 00:00:00
abstract：OBJECTIVES:To evaluate the effectiveness and safety of a new purified collagen gel with specific properties for renal artery embolization in an experimental model in dogs. METHODS:The new gel was prepared from type I bovine tendon collagen, highly purified to eliminate immunogenicity, and reconstituted through extensi...
journal_title：Urology
pub_type： 杂志文章
doi：10.1016/j.urology.2005.10.043
更新日期：2006-04-01 00:00:00
