capB2 Expression Is Associated with Staphylococcus aureus Pathogenicity.

Abstract:

:CapB2 is recognized as a tyrosine kinase and is likely a vital factor in extracellular polysaccharide synthesis in Staphylococcus aureus, but its pathogenicity function and regulatory mechanism remain obscure. Here, we demonstrate that CapB2 enhances bacterial virulence in a murine model. Mice infected with the wild type SA75 strain exhibited significantly larger (P < 0.05) skin lesions from days 4 to 7 of infection than those challenged with the capB2 mutant strain. The effect on virulence was reverted by restoring the capB2 mutation to the wild type. The related components of the wild type SA75 cell wall in the capB2 mutant strain (SA75ΔcapB2) were thinner than wild type SA75 strain and the capB2 mutant complemented strain (SA75ΔcapB2-C), which was determined by the transmission electron microscopy. The survival percentages of the wild type strain SA75 and SA75ΔcapB2-C were significantly higher relative to SA75ΔcapB2. The results of qRT-PCR studies also indicated that mutations in regulatory gene sarA led to a drastic increase in capB2 gene transcription, with a 326-fold increase of growth at 6 h compared with the wild type strain, suggesting that sarA is a major negative regulator of capB2 expression. Taken together, these results demonstrate that the expression of CapB2 promotes S. aureus virulence in a mouse model of skin infection, and that capB2 gene transcription is regulated negatively by SarA.

journal_name

Front Microbiol

authors

Li D,Guo Y,Wang S,Lv J,Qi X,Chen Z,Han L,Zhang X,Wang L,Yu F

doi

10.3389/fmicb.2017.00184

subject

Has Abstract

pub_date

2017-02-10 00:00:00

pages

184

issn

1664-302X

journal_volume

8

pub_type

杂志文章
  • Chloroplast symbiosis in a marine ciliate: ecophysiology and the risks and rewards of hosting foreign organelles.

    abstract::Simultaneous use of both heterotrophic and autotrophic metabolism ("mixotrophy") is common among protists. Strombidium rassoulzadegani is a planktonic mixotrophic marine ciliate that saves chloroplasts from its algal food and obtains a nutritional subsidy via photosynthesis. Cultures from the northeast, northwest, and...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00321

    authors: McManus GB,Schoener DM,Haberlandt K

    更新日期:2012-09-04 00:00:00

  • Environmental bacteriophages: viruses of microbes in aquatic ecosystems.

    abstract::Since the discovery 2-3 decades ago that viruses of microbes are abundant in marine ecosystems, viral ecology has grown increasingly to reach the status of a full scientific discipline in environmental sciences. A dedicated ISVM society, the International Society for Viruses of Microorganisms, (http://www.isvm.org/) w...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2014.00355

    authors: Sime-Ngando T

    更新日期:2014-07-24 00:00:00

  • The dynamic nature and territory of transcriptional machinery in the bacterial chromosome.

    abstract::Our knowledge of the regulation of genes involved in bacterial growth and stress responses is extensive; however, we have only recently begun to understand how environmental cues influence the dynamic, three-dimensional distribution of RNA polymerase (RNAP) in Escherichia coli on the level of single cell, using wide-f...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.00497

    authors: Jin DJ,Cagliero C,Martin CM,Izard J,Zhou YN

    更新日期:2015-05-21 00:00:00

  • Detection of autotrophic verrucomicrobial methanotrophs in a geothermal environment using stable isotope probing.

    abstract::Genomic analysis of the methanotrophic verrucomicrobium "Methylacidiphilum infernorum" strain V4 has shown that most pathways conferring its methanotrophic lifestyle are similar to those found in proteobacterial methanotrophs. However, due to the large sequence divergence of its methane monooxygenase-encoding genes (p...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00303

    authors: Sharp CE,Stott MB,Dunfield PF

    更新日期:2012-08-17 00:00:00

  • Microbial Eukaryote Diversity and Activity in the Water Column of the South China Sea Based on DNA and RNA High Throughput Sequencing.

    abstract::To study the diversity and metabolic activity of microbial eukaryotes in the water column of the South China Sea, genomic DNA and RNA were co-extracted from samples collected down to bathyal depth at two sites. V9 regions of both SSU rRNA gene and its transcript (cDNA) were amplified and sequenced using high throughpu...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01121

    authors: Xu D,Li R,Hu C,Sun P,Jiao N,Warren A

    更新日期:2017-06-14 00:00:00

  • Frontiers in Plant Breeding: Perspectives for the Selection of Vegetables Less Susceptible to Enteric Pathogens.

    abstract::Fresh vegetables including baby greens, microgreens, and sprouts can host human pathogens without exhibiting any visible signs of spoilage. It is clear that the vast majority of foodborne disease outbreaks associated with vegetable produce are not simply a result of an oversight by a producer, as it was shown that zoo...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2020.01087

    authors: Henriquez T,Lenzi A,Baldi A,Marvasi M

    更新日期:2020-05-28 00:00:00

  • Corrigendum: Living at the Extremes: Extremophiles and the Limits of Life in a Planetary Context.

    abstract::[This corrects the article DOI: 10.3389/fmicb.2019.00780.]. ...

    journal_title:Frontiers in microbiology

    pub_type: 已发布勘误

    doi:10.3389/fmicb.2019.01785

    authors: Merino N,Aronson HS,Bojanova DP,Feyhl-Buska J,Wong ML,Zhang S,Giovannelli D

    更新日期:2019-08-13 00:00:00

  • Two-Stage Interpretation of Changes in TEER of Intestinal Epithelial Layers Protected by Adhering Bifidobacteria During E. coli Challenges.

    abstract::Mechanisms of gastrointestinal protection by probiotic bacteria against infection involve amongst others, modulation of intestinal epithelial barrier function. Trans-epithelial electrical resistance (TEER) is widely used to evaluate cellular barrier functions. Here, we developed a two-stage interpretative model of the...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.599555

    authors: Yuan L,van der Mei HC,Busscher HJ,Peterson BW

    更新日期:2020-11-19 00:00:00

  • A PAS domain-containing regulator controls flagella-flagella interactions in Campylobacter jejuni.

    abstract::The bipolar flagella of the foodborne bacterial pathogen Campylobacter jejuni confer motility, which is essential for virulence. The flagella of C. jejuni are post-translationally modified, but how this process is controlled is not well understood. In this work, we have identified a novel PAS-domain containing regulat...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00770

    authors: Reuter M,Periago PM,Mulholland F,Brown HL,van Vliet AH

    更新日期:2015-07-30 00:00:00

  • Modulation of Plant and Fungal Gene Expression Upon Cd Exposure and Symbiosis in Ericoid Mycorrhizal Vaccinium myrtillus.

    abstract::The success of Ericaceae in stressful habitats enriched in heavy metals has been ascribed to the distinctive abilities of their mycorrhizal fungal partners to withstand heavy metal stress and to enhance metal tolerance in the host plant. Whereas heavy metal tolerance has been extensively investigated in some ericoid m...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00341

    authors: Casarrubia S,Martino E,Daghino S,Kohler A,Morin E,Khouja HR,Murat C,Barry KW,Lindquist EA,Martin FM,Perotto S

    更新日期:2020-03-09 00:00:00

  • From Vineyard Soil to Wine Fermentation: Microbiome Approximations to Explain the "terroir" Concept.

    abstract::Wine originally emerged as a serendipitous mix of chemistry and biology, where microorganisms played a decisive role. From these ancient fermentations to the current monitored industrial processes, winegrowers and winemakers have been continuously changing their practices according to scientific knowledge and advances...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2017.00821

    authors: Belda I,Zarraonaindia I,Perisin M,Palacios A,Acedo A

    更新日期:2017-05-08 00:00:00

  • Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry-Based Metabolomics Reveals Metabolic Alterations in the Mouse Cerebellum During Toxoplasma gondii Infection.

    abstract::Toxoplasma gondii is a protozoan parasite with a remarkable neurotropism. We recently showed that T. gondii infection can alter the global metabolism of the cerebral cortex of mice. However, the impact of T. gondii infection on the metabolism of the cerebellum remains unknown. Here we apply metabolomic profiling to di...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01555

    authors: Ma J,He JJ,Hou JL,Zhou CX,Elsheikha HM,Zhu XQ

    更新日期:2020-07-10 00:00:00

  • The Woodrat Gut Microbiota as an Experimental System for Understanding Microbial Metabolism of Dietary Toxins.

    abstract::The microbial communities inhabiting the alimentary tracts of mammals, particularly those of herbivores, are estimated to be one of the densest microbial reservoirs on Earth. The significance of these gut microbes in influencing the physiology, ecology and evolution of their hosts is only beginning to be realized. To ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.01165

    authors: Kohl KD,Dearing MD

    更新日期:2016-07-28 00:00:00

  • Structural and molecular determinants of HIV-1 Gag binding to the plasma membrane.

    abstract::Targeting of the Gag polyprotein to the plasma membrane (PM) for assembly is a critical event in the late phase of immunodeficiency virus type-1 (HIV-1) infection. Gag binding to the PM is mediated by interactions between the myristoylated matrix (MA) domain and PM lipids. Despite the extensive biochemical and in vitr...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00232

    authors: Vlach J,Saad JS

    更新日期:2015-03-20 00:00:00

  • Cathelicidin-Derived Synthetic Peptide Improves Therapeutic Potential of Vancomycin Against Pseudomonas aeruginosa.

    abstract::Pseudomonas aeruginosa (PA) is the leading cause of corneal blindness worldwide. A constant increase in multi-drug resistant PA strains have heightened the challenge of effectively managing corneal infections with conventional antibiotics. Antimicrobial peptides are promising antibiotic analogs with a unique mode of a...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02190

    authors: Mohammed I,Said DG,Nubile M,Mastropasqua L,Dua HS

    更新日期:2019-09-19 00:00:00

  • Cell Cycle Regulatory Functions of the KSHV Oncoprotein LANA.

    abstract::Manipulation of cell cycle is a commonly employed strategy of viruses for achieving a favorable cellular environment during infection. Kaposi's sarcoma-associated herpesvirus (KSHV), the primary etiological agent of several human malignancies including Kaposi's sarcoma, and primary effusion lymphoma, encodes several o...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.00334

    authors: Wei F,Gan J,Wang C,Zhu C,Cai Q

    更新日期:2016-03-30 00:00:00

  • Focused Review: Cytotoxic and Antioxidant Potentials of Mangrove-Derived Streptomyces.

    abstract::Human life expectancy is rapidly increasing with an associated increasing burden of chronic diseases, such as neurodegenerative diseases and cancer. However, there is limited progress in finding effective treatment for these conditions. For this reason, members of the genus Streptomyces have been explored extensively ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2017.02065

    authors: Ser HL,Tan LT,Law JW,Chan KG,Duangjai A,Saokaew S,Pusparajah P,Ab Mutalib NS,Khan TM,Goh BH,Lee LH

    更新日期:2017-11-01 00:00:00

  • The Biotherapeutic Potential of Lactobacillus reuteri Characterized Using a Target-Specific Selection Process.

    abstract::A growing body of clinical and experimental data supports the view that the efficacy of probiotics is strain-specific and restricted to particular pathological conditions, which means that newly isolated probiotic strains need to be targeted to a specific disease. Following national and international guidelines, we us...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00532

    authors: Sagheddu V,Uggeri F,Belogi L,Remollino L,Brun P,Bernabè G,Moretti G,Porzionato A,Morelli L,Castagliuolo I,Elli M

    更新日期:2020-04-15 00:00:00

  • Intra-Species and Inter-Kingdom Signaling of Legionella pneumophila.

    abstract::The ubiquitous Gram-negative bacterium Legionella pneumophila parasitizes environ mental amoebae and, upon inhalation, replicates in alveolar macrophages, thus causing a life-threatening pneumonia called "Legionnaires' disease." The opportunistic pathogen employs a bi-phasic life cycle, alternating between a replicati...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2017.00079

    authors: Hochstrasser R,Hilbi H

    更新日期:2017-02-03 00:00:00

  • Omeprazole Increases the Efficacy of Acyclovir Against Herpes Simplex Virus Type 1 and 2.

    abstract::Omeprazole was shown to improve the anti-cancer effects of the nucleoside analogue 5-fluorouracil. Here, we combined omeprazole with the antiviral nucleoside analogues ribavirin and acyclovir. Omeprazole did not affect the antiviral effects of ribavirin in non-toxic concentrations up to 80 μg/mL but increased the acyc...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02790

    authors: Michaelis M,Kleinschmidt MC,Bojkova D,Rabenau HF,Wass MN,Cinatl J Jr

    更新日期:2019-12-03 00:00:00

  • Non-coding RNAs in Host-Pathogen Interactions: Subversion of Mammalian Cell Functions by Protozoan Parasites.

    abstract::Pathogens have evolved mechanisms to modulate host cell functions and avoid recognition and destruction by the host damage response. For many years, researchers have focused on proteins as the main effectors used by pathogens to hijack host cell pathways, but only recently with the development of deep RNA sequencing t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2017.00474

    authors: Bayer-Santos E,Marini MM,da Silveira JF

    更新日期:2017-03-21 00:00:00

  • Physiological Studies of Glutamine Synthetases I and III from Synechococcus sp. WH7803 Reveal Differential Regulation.

    abstract::The marine picocyanobacterium Synechococcus sp. WH7803 possesses two glutamine synthetases (GSs; EC 6.3.1.2), GSI encoded by glnA and GSIII encoded by glnN. This is the first work addressing the physiological regulation of both enzymes in a marine cyanobacterial strain. The increase of GS activity upon nitrogen starva...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00969

    authors: Domínguez-Martín MA,Díez J,García-Fernández JM

    更新日期:2016-06-28 00:00:00

  • Anchors for effectors: subversion of phosphoinositide lipids by legionella.

    abstract::The facultative intracellular pathogen Legionella pneumophila replicates in free-living amoebae and macrophages within a distinct compartment, the "Legionella-containing vacuole" (LCV). LCV formation involves phosphoinositide (PI) glycerolipids, which are key factors controlling vesicle trafficking pathways and membra...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2011.00091

    authors: Hilbi H,Weber S,Finsel I

    更新日期:2011-04-27 00:00:00

  • Hydrogen Sulfide From Cysteine Desulfurase, Not 3-Mercaptopyruvate Sulfurtransferase, Contributes to Sustaining Cell Growth and Bioenergetics in E. coli Under Anaerobic Conditions.

    abstract::Endogenous hydrogen sulfide (H2S), which is primarily generated by 3-mercaptopyruvate sulfurtransferase (3-MST) in Escherichia coli (E. coli) under aerobic conditions, renders bacteria highly resistant to oxidative stress. However, the biosynthetic pathway and physiological role of this gas under anaerobic conditions ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02357

    authors: Wang J,Guo X,Li H,Qi H,Qian J,Yan S,Shi J,Niu W

    更新日期:2019-10-11 00:00:00

  • Links between Soil Fungal Diversity and Plant and Soil Properties on the Loess Plateau.

    abstract::Previous studies have revealed inconsistent correlations between fungal diversity and plant/soil properties from local to global scales. Here, we investigated the internal relationships between soil fungal diversity and plant/soil properties on the Loess Plateau following vegetation restoration, using Illumina sequenc...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02198

    authors: Yang Y,Dou Y,Huang Y,An S

    更新日期:2017-11-07 00:00:00

  • Prevalence and characteristics of extended-spectrum β-lactamase genes in Escherichia coli isolated from piglets with post-weaning diarrhea in Heilongjiang province, China.

    abstract:OBJECTIVES:The purpose of this study was to investigate the prevalence of extended spectrum β-lactamase (ESBL) genes in Escherichia coli isolated from post-weaning diarrhea (PWD) piglets in Heilongjiang province, China. METHODS:Of 458 E. coli isolated from 589 fecal samples from PWD piglets, a total of 198 isolates we...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01103

    authors: Xu G,An W,Wang H,Zhang X

    更新日期:2015-10-08 00:00:00

  • Dynamic Alterations in Yak Rumen Bacteria Community and Metabolome Characteristics in Response to Feed Type.

    abstract::Current knowledge about the relationships between ruminal bacterial communities and metabolite profiles in the yak rumen is limited. This is due to differences in the nutritional and metabolic features between yak and other ordinary cattle combined with difficulties associated with farm-based research and a lack of te...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01116

    authors: Liu C,Wu H,Liu S,Chai S,Meng Q,Zhou Z

    更新日期:2019-05-22 00:00:00

  • Supplemental Plant Extracts From Flos lonicerae in Combination With Baikal skullcap Attenuate Intestinal Disruption and Modulate Gut Microbiota in Laying Hens Challenged by Salmonella pullorum.

    abstract::Dietary inclusions of baicalin and chlorogenic acid were beneficial for intestinal health in pigs. Nevertheless, it is unknown whether these plant-derived products had protection for intestine against bacterial challenge in chickens. This study was aimed at evaluating the potential mitigating effects of plant extracts...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01681

    authors: Wang WW,Jia HJ,Zhang HJ,Wang J,Lv HY,Wu SG,Qi GH

    更新日期:2019-07-24 00:00:00

  • Comparison of methods for the enumeration of enterohemorrhagic Escherichia coli from veal hides and carcasses.

    abstract::The increased association of enterohemorrhagic Escherichia coli (EHEC) with veal calves has led the United States Department of Agriculture Food Safety and Inspection Service to report results of veal meat contaminated with the Top 7 serogroups separately from beef cattle. However, detection methods that can also prov...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01062

    authors: Luedtke BE,Bosilevac JM

    更新日期:2015-09-29 00:00:00

  • Effects of Polymorphisms in the SjSP-13 Gene of Schistosoma japonicum on Its Diagnostic Efficacy and Immunogenicity.

    abstract::Schistosomiasis japonica is one of the most prevalent parasitic diseases in China. The scarcity of effective diagnostic tools is a major factor that contributes to the high prevalence of schistosomiasis japonica. SjSP-13 is a promising serological diagnostic biomarker of the disease. However, it is unclear whether pol...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01695

    authors: Xu X,Cui X,Zhu L,Li Z,Zhang Y,Ma L,Pan W

    更新日期:2018-07-25 00:00:00