Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry-Based Metabolomics Reveals Metabolic Alterations in the Mouse Cerebellum During Toxoplasma gondii Infection.

Abstract:

:Toxoplasma gondii is a protozoan parasite with a remarkable neurotropism. We recently showed that T. gondii infection can alter the global metabolism of the cerebral cortex of mice. However, the impact of T. gondii infection on the metabolism of the cerebellum remains unknown. Here we apply metabolomic profiling to discover metabolic changes associated with T. gondii infection of the mouse cerebellum using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Multivariate statistics revealed differences in the metabolic profiles between the infected and control mouse groups and between the infected mouse groups as infection advanced. We also detected 10, 22, and 42 significantly altered metabolites (SAMs) in the infected cerebellum at 7, 14, and 21 days post infection (dpi), respectively. Four metabolites [tabersonine, arachidonic acid (AA), docosahexaenoic acid, and oleic acid] were identified as potential biomarker or responsive metabolites to T. gondii infection in the mouse cerebellum. Three of these metabolites (AA, docosahexaenoic acid, and oleic acid) play roles in the regulation of host behavior and immune response. Pathway analysis showed that T. gondii infection of the cerebellum involves reprogramming of amino acid and lipid metabolism. These results showcase temporal metabolomic changes during cerebellar infection by T. gondii in mice. The study provides new insight into the neuropathogenesis of T. gondii infection and reveals new metabolites and pathways that mediate the interplay between T. gondii and the mouse cerebellum.

journal_name

Front Microbiol

authors

Ma J,He JJ,Hou JL,Zhou CX,Elsheikha HM,Zhu XQ

doi

10.3389/fmicb.2020.01555

subject

Has Abstract

pub_date

2020-07-10 00:00:00

pages

1555

issn

1664-302X

journal_volume

11

pub_type

杂志文章
  • Relevance of GC content to the conservation of DNA polymerase III/mismatch repair system in Gram-positive bacteria.

    abstract::The mechanism of DNA replication is one of the driving forces of genome evolution. Bacterial DNA polymerase III, the primary complex of DNA replication, consists of PolC and DnaE. PolC is conserved in Gram-positive bacteria, especially in the Firmicutes with low GC content, whereas DnaE is widely conserved in most Gra...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2013.00266

    authors: Akashi M,Yoshikawa H

    更新日期:2013-09-17 00:00:00

  • Mass Spectrometry Guided Discovery and Design of Novel Asperphenamate Analogs From Penicillium astrolabium Reveals an Extraordinary NRPS Flexibility.

    abstract::Asperphenamate is a small peptide natural product that has gained much interest due to its antitumor activity. In the recent years numerous bioactive synthetic asperphenamate analogs have been reported, whereas only a handful of natural analogs either of microbial or plant origin has been discovered. Herein we describ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.618730

    authors: Subko K,Wang X,Nielsen FH,Isbrandt T,Gotfredsen CH,Ramos MC,Mackenzie T,Vicente F,Genilloud O,Frisvad JC,Larsen TO

    更新日期:2021-01-15 00:00:00

  • Diversity of Sinorhizobium (Ensifer) meliloti Bacteriophages in the Rhizosphere of Medicago marina: Myoviruses, Filamentous and N4-Like Podovirus.

    abstract::Using different Sinorhizobium meliloti strains as hosts, we isolated eight new virulent phages from the rhizosphere of the coastal legume Medicago marina. Half of the isolated phages showed a very narrow host range while the other half exhibited a wider host range within the strains tested. Electron microscopy studies...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00022

    authors: Cubo MT,Alías-Villegas C,Balsanelli E,Mesa D,de Souza E,Espuny MR

    更新日期:2020-01-24 00:00:00

  • Corrigendum: Shifts in metabolic hydrogen sinks in the methanogenesis-inhibited ruminal fermentation: a meta-analysis.

    abstract::[This corrects the article on p. 37 in vol. 6, PMID: 25699029.]. ...

    journal_title:Frontiers in microbiology

    pub_type: 已发布勘误

    doi:10.3389/fmicb.2015.00538

    authors: Ungerfeld EM

    更新日期:2015-06-02 00:00:00

  • Seasonal and Spatial Variations in Bacterial Communities From Tetrodotoxin-Bearing and Non-tetrodotoxin-Bearing Clams.

    abstract::Tetrodotoxin (TTX) is one of the most potent naturally occurring compounds and is responsible for many human intoxications worldwide. Paphies australis are endemic clams to New Zealand which contain varying concentrations of TTX. Research suggests that P. australis accumulate the toxin exogenously, but the source rema...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01860

    authors: Biessy L,Pearman JK,Smith KF,Hawes I,Wood SA

    更新日期:2020-08-05 00:00:00

  • Phylogeography and Ecological Niche Shape the Cichlid Fish Gut Microbiota in Central American and African Lakes.

    abstract::Cichlid fishes, with their repeated colonization of lakes and subsequent radiations at different scales of phylogenetic and ecological diversification, offer an excellent model system to understand the factors shaping the host-gut microbiota association in nature. Here, we characterized the gut microbiota of the Amphi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02372

    authors: Baldo L,Riera JL,Salzburger W,Barluenga M

    更新日期:2019-10-15 00:00:00

  • Applications of High and Ultra High Pressure Homogenization for Food Safety.

    abstract::Traditionally, the shelf-life and safety of foods have been achieved by thermal processing. Low temperature long time and high temperature short time treatments are the most commonly used hurdles for the pasteurization of fluid foods and raw materials. However, the thermal treatments can reduce the product quality and...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.01132

    authors: Patrignani F,Lanciotti R

    更新日期:2016-08-03 00:00:00

  • Genomic analysis of six new Geobacillus strains reveals highly conserved carbohydrate degradation architectures and strategies.

    abstract::In this work we report the whole genome sequences of six new Geobacillus xylanolytic strains along with the genomic analysis of their capability to degrade carbohydrates. The six sequenced Geobacillus strains described here have a range of GC contents from 43.9% to 52.5% and clade with named Geobacillus species throug...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00430

    authors: Brumm PJ,De Maayer P,Mead DA,Cowan DA

    更新日期:2015-05-12 00:00:00

  • Fold modulating function: bacterial toxins to functional amyloids.

    abstract::Many bacteria produce cytolytic toxins that target host cells or other competing microbes. It is well known that environmental factors control toxin expression, however, recent work suggests that some bacteria manipulate the fold of these protein toxins to control their function. The β-sheet rich amyloid fold is a hig...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2014.00401

    authors: Syed AK,Boles BR

    更新日期:2014-08-01 00:00:00

  • Endosymbionts Alter Larva-to-Nymph Transstadial Transmission of Babesia microti in Rhipicephalus haemaphysaloides Ticks.

    abstract::Maternally inherited endosymbionts inhabit a variety of arthropods. Some of them can protect the arthropod host against a wide range of pathogens. However, very little is known about the association between endosymbionts and pathogen susceptibility in ticks. The present study investigated the effect of endosymbionts o...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01415

    authors: Li LH,Zhang Y,Zhu D,Zhou XN

    更新日期:2018-06-27 00:00:00

  • Responses to Hydric Stress in the Seed-Borne Necrotrophic Fungus Alternaria brassicicola.

    abstract::Alternaria brassicicola is a necrotrophic fungus causing black spot disease and is an economically important seed-borne pathogen of cultivated brassicas. Seed transmission is a crucial component of its parasitic cycle as it promotes long-term survival and dispersal. Recent studies, conducted with the Arabidopsis thali...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01969

    authors: N'Guyen GQ,Raulo R,Marchi M,Agustí-Brisach C,Iacomi B,Pelletier S,Renou JP,Bataillé-Simoneau N,Campion C,Bastide F,Hamon B,Mouchès C,Porcheron B,Lemoine R,Kwasiborski A,Simoneau P,Guillemette T

    更新日期:2019-08-30 00:00:00

  • Benthic microbial communities of coastal terrestrial and ice shelf Antarctic meltwater ponds.

    abstract::The numerous perennial meltwater ponds distributed throughout Antarctica represent diverse and productive ecosystems central to the ecological functioning of the surrounding ultra oligotrophic environment. The dominant taxa in the pond benthic communities have been well described however, little is known regarding the...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00485

    authors: Archer SD,McDonald IR,Herbold CW,Lee CK,Cary CS

    更新日期:2015-05-27 00:00:00

  • Viruses and Evolution - Viruses First? A Personal Perspective.

    abstract::The discovery of exoplanets within putative habitable zones revolutionized astrobiology in recent years. It stimulated interest in the question about the origin of life and its evolution. Here, we discuss what the roles of viruses might have been at the beginning of life and during evolution. Viruses are the most abun...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00523

    authors: Moelling K,Broecker F

    更新日期:2019-03-19 00:00:00

  • Strong Inference in Mathematical Modeling: A Method for Robust Science in the Twenty-First Century.

    abstract::While there are many opinions on what mathematical modeling in biology is, in essence, modeling is a mathematical tool, like a microscope, which allows consequences to logically follow from a set of assumptions. Only when this tool is applied appropriately, as microscope is used to look at small items, it may allow to...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01131

    authors: Ganusov VV

    更新日期:2016-07-22 00:00:00

  • Factors influencing the diversity of iron uptake systems in aquatic microorganisms.

    abstract::Iron (Fe) is an essential micronutrient for many processes in all living cells. Dissolved Fe (dFe) concentrations in the ocean are of the order of a few nM, and Fe is often a factor limiting primary production. Bioavailability of Fe in aquatic environments is believed to be primarily controlled through chelation by Fe...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00362

    authors: Desai DK,Desai FD,Laroche J

    更新日期:2012-10-18 00:00:00

  • Mechanism of Action of Surface Immobilized Antimicrobial Peptides Against Pseudomonas aeruginosa.

    abstract::Bacterial colonization and biofilm development on medical devices can lead to infection. Antimicrobial peptide-coated surfaces may prevent such infections. Melimine and Mel4 are chimeric cationic peptides showing broad-spectrum antimicrobial activity once attached to biomaterials and are highly biocompatible in animal...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.03053

    authors: Yasir M,Dutta D,Hossain KR,Chen R,Ho KKK,Kuppusamy R,Clarke RJ,Kumar N,Willcox MDP

    更新日期:2020-01-22 00:00:00

  • Genetic Characterization of a bla VIM-24-Carrying IncP-7β Plasmid p1160-VIM and a bla VIM-4-Harboring Integrative and Conjugative Element Tn6413 From Clinical Pseudomonas aeruginosa.

    abstract::This study presents three novel integrons In1394, In1395, and In1443, three novel unit transposons Tn6392, Tn6393, and Tn6403, one novel conjugative element (ICE) Tn6413, and the first sequenced IncP-7 resistance plasmid p1160-VIM from clinical Pseudomonas aeruginosa. Detailed sequence comparison of p1160-VIM (carryin...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00213

    authors: Zeng L,Zhan Z,Hu L,Jiang X,Zhang Y,Feng J,Gao B,Zhao Y,Yang W,Yang H,Yin Z,Zhou D

    更新日期:2019-02-26 00:00:00

  • Computational Exploration of Putative LuxR Solos in Archaea and Their Functional Implications in Quorum Sensing.

    abstract::LuxR solos are unexplored in Archaea, despite their vital role in the bacterial regulatory network. They assist bacteria in perceiving acyl homoserine lactones (AHLs) and/or non-AHLs signaling molecules for establishing intraspecies, interspecies, and interkingdom communication. In this study, we explored the potentia...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00798

    authors: Rajput A,Kumar M

    更新日期:2017-05-03 00:00:00

  • The AirSR two-component system contributes to Staphylococcus aureus survival in human blood and transcriptionally regulates sspABC operon.

    abstract::To date, genes identified and transcriptionally regulated by the AirSR TCS have been involved in energy production and cellular homeostasis of the staphylococcal cell. It is well accepted that the state of cellular metabolism impacts the expression of virulence factors in Staphylococcus aureus. For this reason, we con...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00682

    authors: Hall JW,Yang J,Guo H,Ji Y

    更新日期:2015-07-03 00:00:00

  • Infectious Agents As Markers of Human Migration toward the Amazon Region of Brazil.

    abstract::Infectious agents are common companions of humans and since ancient times they follow human migration on their search for a better place to live. The study of paleomicrobiology was significantly improved in its accuracy of measurement with the constant development of better methods to detect and analyze nucleic acids....

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2017.01663

    authors: Ishak R,Machado LFA,Cayres-Vallinoto I,Guimarães Ishak MO,Vallinoto ACR

    更新日期:2017-08-31 00:00:00

  • Differentiated Mechanisms of Biochar Mitigating Straw-Induced Greenhouse Gas Emissions in Two Contrasting Paddy Soils.

    abstract::Straw returns to the soil is an effective way to improve soil organic carbon and reduce air pollution by straw burning, but this may increase CH4 and N2O emissions risks in paddy soils. Biochar has been used as a soil amendment to improve soil fertility and mitigate CH4 and N2O emissions. However, little is known abou...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02566

    authors: Wang YQ,Bai R,Di HJ,Mo LY,Han B,Zhang LM,He JZ

    更新日期:2018-11-13 00:00:00

  • Beyond a Ribosomal RNA Methyltransferase, the Wider Role of MraW in DNA Methylation, Motility and Colonization in Escherichia coli O157:H7.

    abstract::MraW is a 16S rRNA methyltransferase and plays a role in the fine-tuning of the ribosomal decoding center. It was recently found to contribute to the virulence of Staphylococcus aureus. In this study, we examined the function of MraW in Escherichia coli O157:H7 and found that the deletion of mraW led to decreased moti...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02520

    authors: Xu X,Zhang H,Huang Y,Zhang Y,Wu C,Gao P,Teng Z,Luo X,Peng X,Wang X,Wang D,Pu J,Zhao H,Lu X,Lu S,Ye C,Dong Y,Lan R,Xu J

    更新日期:2019-11-13 00:00:00

  • Ascorbic acid-dependent gene expression in Streptococcus pneumoniae and the activator function of the transcriptional regulator UlaR2.

    abstract::In this study, we have explored the impact of ascorbic acid on the transcriptome of Streptococcus pneumoniae D39. The expression of several genes and operons, including the ula operon (which has been previously shown to be involved in ascorbic acid utilization), the AdcR regulon (which has been previously shown to be ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00072

    authors: Afzal M,Shafeeq S,Kuipers OP

    更新日期:2015-02-11 00:00:00

  • Allergies, Helicobacter pylori and the continental enigmas.

    abstract::Helicobacter pylori, a gastric pathogen, is known to be associated with gastric and duodenal ulcers, and is also a strong risk factor for the development of gastric cancer and lymphoma of the mucosal-associated lymphoid tissue. Ordinarily, this should make a strong case for its eradication at par with any other infect...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.00578

    authors: Sitaraman R

    更新日期:2015-06-09 00:00:00

  • Saccharomyces cerevisiae - Insects Association: Impacts, Biogeography, and Extent.

    abstract::Over the last few years, an increasing number of studies have reported the existence of an association between the budding yeast Saccharomyces cerevisiae and insects. The discovery of this relationship has called into question the hypothesis that S. cerevisiae is unable to survive in nature and that the presence of S....

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2020.01629

    authors: Meriggi N,Di Paola M,Cavalieri D,Stefanini I

    更新日期:2020-07-14 00:00:00

  • Molecular Mechanisms of Colistin Resistance in Klebsiella pneumoniae Causing Bacteremia from India-A First Report.

    abstract::Colistin has long been a reserve drug used for the treatment of carbapenem resistant Klebsiella pneumoniae. Carbapenem resistance in K. pneumoniae has been increasing and is as high as 44% in India. Although a reserve agent, with rise in rates of resistance to carbapenems, the usage of colistin has increased over the ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.02135

    authors: Pragasam AK,Shankar C,Veeraraghavan B,Biswas I,Nabarro LE,Inbanathan FY,George B,Verghese S

    更新日期:2017-01-09 00:00:00

  • One System for All: Is Mass Spectrometry a Future Alternative for Conventional Antibiotic Susceptibility Testing?

    abstract::The two main pillars of clinical microbiological diagnostics are the identification of potentially pathogenic microorganisms from patient samples and the testing for antibiotic susceptibility (AST) to allow efficient treatment with active antimicrobial agents. While routine microbial species identification is increasi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2019.02711

    authors: Welker M,van Belkum A

    更新日期:2019-11-26 00:00:00

  • Characterization and Testing the Efficiency of Acinetobacter baumannii Phage vB-GEC_Ab-M-G7 as an Antibacterial Agent.

    abstract::Acinetobacter baumannii is a gram-negative, non-motile bacterium that, due to its multidrug resistance, has become a major nosocomial pathogen. The increasing number of multidrug resistant (MDR) strains has renewed interest in phage therapy. The aim of our study was to assess the effectiveness of phage administration ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01590

    authors: Kusradze I,Karumidze N,Rigvava S,Dvalidze T,Katsitadze M,Amiranashvili I,Goderdzishvili M

    更新日期:2016-10-04 00:00:00

  • Comparison of PCR and Viable Count as a Method for Enumeration of Bacteria in an A/J Mouse Aerosol Model of Q Fever.

    abstract::Historically, disease progression in animal models of Q fever has been carried out using PCR to monitor the presence of Coxiella burnetii DNA in the host. However, the colonization and dissemination of other bacterial infections in animal models are tracked using viable counts, enabling an accurate assessment of viabl...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01552

    authors: Hartley MG,Ralph E,Norville IH,Prior JL,Atkins TP

    更新日期:2019-07-16 00:00:00

  • Composition of Total and Cell-Proliferating Bacterioplankton Community in Early Summer in the North Sea - Roseobacters Are the Most Active Component.

    abstract::Heterotrophic bacterioplankton communities play an important role in organic matter processing in the oceans worldwide. In order to investigate the significance of distinct phylogenetic bacterial groups it is not only important to assess their quantitative abundance but also their growth dynamics in relation to the en...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01771

    authors: Bakenhus I,Dlugosch L,Billerbeck S,Giebel HA,Milke F,Simon M

    更新日期:2017-09-13 00:00:00