Abstract:
BACKGROUND:Chromosome microdissection is one of the most important techniques in molecular cytogenetic research. Cotton (Gossypium Linnaeus, 1753) is the main natural fiber crop in the world. The resistance gene analog (RGA) cloning after its single chromosome microdissection can greatly promote cotton genome research and breeding. RESULTS:Using the linker adaptor PCR (LA-PCR) with the primers of rice disease-resistance homologues, three nucleotide sequences PS016 (KU051681), PS054 (KU051682), and PS157 (KU051680) were obtained from the chromosome Ah01 of upland cotton (cv. TM-1). The Blast results showed that the three sequences are the nucleotide binding site-leucine rich repeat (NBS-LRR) type RGAs. Clustering results indicated that they are homologous to these published RGAs. Thus, the three RGAs can definitely be confirmed as NBS-LRR class of RGAs in upland cotton. CONCLUSIONS:Using single chromosome microdissection technique, DNA libraries containing cotton RGAs were obtained. This technique can promote cotton gene cloning, marker development and even the improvement of cotton genome research and breeding.
journal_name
Hereditasjournal_title
Hereditasauthors
Cao X,Liu Y,Liu Z,Liu F,Wu Y,Zhou Z,Cai X,Wang X,Zhang Z,Wang Y,Luo Z,Peng R,Wang Kdoi
10.1186/s41065-017-0035-3subject
Has Abstractpub_date
2017-05-18 00:00:00pages
13eissn
0018-0661issn
1601-5223pii
35journal_volume
154pub_type
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