Microdissection of the Ah01 chromosome in upland cotton and microcloning of resistance gene anologs from the single chromosome.

Abstract:

BACKGROUND:Chromosome microdissection is one of the most important techniques in molecular cytogenetic research. Cotton (Gossypium Linnaeus, 1753) is the main natural fiber crop in the world. The resistance gene analog (RGA) cloning after its single chromosome microdissection can greatly promote cotton genome research and breeding. RESULTS:Using the linker adaptor PCR (LA-PCR) with the primers of rice disease-resistance homologues, three nucleotide sequences PS016 (KU051681), PS054 (KU051682), and PS157 (KU051680) were obtained from the chromosome Ah01 of upland cotton (cv. TM-1). The Blast results showed that the three sequences are the nucleotide binding site-leucine rich repeat (NBS-LRR) type RGAs. Clustering results indicated that they are homologous to these published RGAs. Thus, the three RGAs can definitely be confirmed as NBS-LRR class of RGAs in upland cotton. CONCLUSIONS:Using single chromosome microdissection technique, DNA libraries containing cotton RGAs were obtained. This technique can promote cotton gene cloning, marker development and even the improvement of cotton genome research and breeding.

journal_name

Hereditas

journal_title

Hereditas

authors

Cao X,Liu Y,Liu Z,Liu F,Wu Y,Zhou Z,Cai X,Wang X,Zhang Z,Wang Y,Luo Z,Peng R,Wang K

doi

10.1186/s41065-017-0035-3

subject

Has Abstract

pub_date

2017-05-18 00:00:00

pages

13

eissn

0018-0661

issn

1601-5223

pii

35

journal_volume

154

pub_type

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