Abstract:
:Nicotine is one of the most abundant and toxic secondary plant metabolites in nature and is defined by high toxicity to plant-feeding insects. Studies suggest that increased expression of cytochrome P450 (CYP6CY3) and the homologous CYP6CY4 genes in Myzus persicae nicotianae is correlated with tolerance to nicotine. Indeed, through expression analyses of the CYP6CY3 and CYP6CY4 genes of different M. persicae subspecies, we determined that the mRNA levels of these two genes were much higher in M. persicae nicotianae than in M. persicae sensu stricto. We hypothesized that the expression of these two genes is subject to post-transcriptional regulation. To investigate the underlying mechanism, the miRNA profile of M. persicae nicotianae was sequenced, and twenty-two miRNAs were predicted to target CYP6CY3. Validation of these miRNAs identified two miRNAs, let-7 and miR-100, whose abundance was highly inversely correlated with the abundance of the CYP6CY3 gene. This result implies that the let-7 and miR-100 miRNAs play a major role in the post-transcriptional regulation of the CYP6CY3 gene. Modulation of the abundance of let-7 and miR-100 through the addition of inhibitors/mimics of let-7 or miR-100 to artificial diet significantly altered the tolerance of M. persicae nicotianae to nicotine, further confirming the regulatory role of these two miRNAs. Interestingly, after decreasing the transcript levels of CYP6CY3 by modulating regulatory miRNAs, the transcript levels of the homologous isozyme CYP6CY4 were significantly elevated, suggesting a compensatory mechanism between the CYP6CY3 gene and its homologous CYP6CY4 gene. Our findings provide insight into the molecular drivers of insect host shifts and reveal an important source of genetic variation for adaptive evolution in insect species.
journal_name
Insect Biochem Mol Bioljournal_title
Insect biochemistry and molecular biologyauthors
Peng T,Pan Y,Gao X,Xi J,Zhang L,Ma K,Wu Y,Zhang J,Shang Qdoi
10.1016/j.ibmb.2016.06.002subject
Has Abstractpub_date
2016-08-01 00:00:00pages
89-97eissn
0965-1748issn
1879-0240pii
S0965-1748(16)30079-0journal_volume
75pub_type
杂志文章abstract::A bioinformatics-based investigation of three insect species with completed genome sequences has revealed that insect chitinase-like proteins (glycosylhydrolase family 18) are encoded by a rather large and diverse group of genes. We identified 16, 16 and 13 putative chitinase-like genes in the genomic databases of the...
journal_title:Insect biochemistry and molecular biology
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journal_title:Insect biochemistry and molecular biology
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doi:10.1016/s0965-1748(98)00019-8
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journal_title:Insect biochemistry and molecular biology
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journal_title:Insect biochemistry and molecular biology
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章,评审
doi:10.1016/j.ibmb.2004.03.021
更新日期:2004-07-01 00:00:00
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2014.10.002
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pub_type: 历史文章,杂志文章,评审
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journal_title:Insect biochemistry and molecular biology
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章,评审
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pub_type: 杂志文章
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/s0965-1748(00)00025-4
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1016/j.ibmb.2011.08.005
更新日期:2011-11-01 00:00:00