Abstract:
:We describe a simple method for evaluating the inhibition of collagen IV degradation by cathepsin B with a surface plasmon resonance (SPR) biosensor. The change in the SPR signal decreased with an increase in the concentration of cathepsin B inhibitors. The order of the inhibitory constant (Ki) obtained by the SPR method was CA074Me≈Z-Phe-Phe-FMK < leupeptin. This order was different from that obtained by benzyloxycarbonyl-Phe-Phe-Fluoromethylketone (Z-Phe-Phe-FMK) as a peptide substrate. The comparison of Ki suggested that CA074 and Z-Phe-Phe-FMK inhibited exopeptidase activity, and leupeptin inhibited the endopeptidase activity of cathepsin B more strongly.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Shoji A,Suenaga Y,Hosaka A,Ishida Y,Yanagida A,Sugawara Mdoi
10.1016/j.jpba.2017.06.026subject
Has Abstractpub_date
2017-10-25 00:00:00pages
79-83eissn
0731-7085issn
1873-264Xpii
S0731-7085(17)30107-3journal_volume
145pub_type
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