Abstract:
:BackgroundHyperoxic lung injury is characterized by cellular damage from high oxygen concentrations that lead to an inflammatory response and it disrupts normal alveolarization in the developing newborn lung. Adenosine is a signaling molecule that is generated extracellularly by ecto-5'-nucleotidase (CD73) in response to injury. Extracellular adenosine signals through cell surface receptors and has been found to have a protective role in acute injury situations; however, chronic elevations have been associated with detrimental changes in chronic lung diseases. We hypothesized that hyperoxia-induced lung injury leads to CD73-mediated increases in extracellular adenosine, which are detrimental to the newborn lung.MethodsC57Bl/6 and CD73-/- mice were exposed to 95% oxygen, 70% oxygen, or room air. Adenosine concentration and markers of pulmonary inflammation and lung development were measured.ResultsExposure to hyperoxia caused pulmonary inflammation and disrupted normal alveolar development in association with increased pulmonary adenosine levels. Loss of CD73-mediated extracellular adenosine production led to decreased survival with exposure to 95% oxygen, and exacerbated pulmonary inflammation and worsened lung development with 70% oxygen exposure.ConclusionExposure to hyperoxia causes lung injury associated with an increase in adenosine concentration, and loss of CD73-mediated adenosine production leads to worsening of hyperoxic lung injury.Pediatric Research advance online publication, 23 August 2017; doi:10.1038/pr.2017.176.
journal_name
Pediatr Resjournal_title
Pediatric researchauthors
Li H,Karmouty-Quintana H,Chen NY,Mills T,Molina J,Blackburn MR,Davies Jdoi
10.1038/pr.2017.176subject
Has Abstractpub_date
2017-08-23 00:00:00eissn
0031-3998issn
1530-0447pii
pr2017176pub_type
杂志文章abstract::The neonate conserves sodium avidly, and sodium intake is normally limited to that present in maternal milk. To evaluate the role of atrial natriuretic peptide (ANP) in this adaptation, preweaned rat pups were artificially reared and fed a formula with either normal sodium (25 mEq/L) or high sodium (145 mEq/L) for 7-8...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199608000-00017
更新日期:1996-08-01 00:00:00
abstract::We investigated the role of circulating bone marrow cells (BMC) in cardiomyocyte regeneration. BMC, isolated from transgenic mice expressing enhanced green fluorescent protein (GFP), were transplanted into lethally irradiated C57BL6 mice. Five weeks after bone marrow transplantation (BMT), flow cytometric analysis for...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.PDR.0000078275.14079.77
更新日期:2003-09-01 00:00:00
abstract::Antibodies of restricted specificity have been identified in the human in response to certain antigens. The present study analyzed tetanus and diphtheria antitoxins isolated from selected human sera and suggested a restricted response in antibody production to each of these antigens. Purified antibodies from eight ser...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-197712000-00009
更新日期:1977-12-01 00:00:00
abstract::A new animal experimental model of human neuroblastoma is described. The model involves xenotransplantation of a poorly differentiated human neuroblastoma cell line (SH-SY5Y) to the subcutaneous tissue in the hind leg of nude rats (WAG mu/rnu). Injection of 20 million cells suspended in 0.2 mL of medium in each hind l...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199703000-00004
更新日期:1997-03-01 00:00:00
abstract::Serum insulin, growth hormone (GH), insulin-like growth factors (IGFs) I and II, cortisol, and albumin concentrations were measured in 15 children with kwashiorkor, 15 with marasmic-kwashiorkor, and 21 with marasmus, before and in the survivors, after nutritional rehabilitation, as well as in 10 underweight and eight ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198611000-00012
更新日期:1986-11-01 00:00:00
abstract::Lipase activities were measured at pH 4 and pH 8 in the placentas of rats made diabetic by streptozotocin treatment and also in the placentas of women classified as having 1) impaired glucose tolerance or type 2 diabetes, 2) type 1 diabetes with no associated vascular complication, and 3) type 1 diabetes with associat...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199112000-00009
更新日期:1991-12-01 00:00:00
abstract::Disorders affecting fetal growth are commonly associated with premature birth. IGFs and their binding proteins (IGFBPs) are potent regulators of fetal growth. In vitro evidence suggests that they regulate collagen turnover. Collagen turnover can be monitored by serum markers of type I collagen synthesis (PINP) and deg...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-200104000-00008
更新日期:2001-04-01 00:00:00
abstract::Sudden infant death syndrome (SIDS) is multifactorial and may result from the interaction of a number of environmental, genetic, and developmental factors. We studied three major genes causing long QT syndrome in 42 Japanese SIDS victims and found five mutations, KCNQ1-K598R, KCNH2-T895M, SCN5A-F532C, SCN5A-G1084S, an...
journal_title:Pediatric research
pub_type: 杂志文章,多中心研究
doi:10.1203/PDR.0b013e3181841eca
更新日期:2008-11-01 00:00:00
abstract::Nitric oxide plays a major role in vascular tone control. Increased blood levels of bradykinin (BK), which stimulates nitric oxide biosynthesis, occur at birth. BK effects on ductus arteriosus (DA) tone were investigated in fetal rabbit under fetal (2.5% O2 "low PO2") and neonatal (30% O2 "high PO2") conditions using ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199904010-00017
更新日期:1999-04-01 00:00:00
abstract::Increased fetal lung expansion profoundly inhibits surfactant protein gene expression and stimulates cellular proliferation in the fetal lung. Our aim was to determine whether increased expansion of the lung after birth, by the application of a continuous positive airway pressure (CPAP) for 12 h, inhibits surfactant p...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-200112000-00015
更新日期:2001-12-01 00:00:00
abstract:BACKGROUND:Pulmonary hypertension (PH) in patients with bronchopulmonary dysplasia (BPD) results from vasoconstriction and/or vascular remodeling, which can be regulated by mitogen-activated protein kinases (MAPKs). MAPKs are deactivated by dual-specificity phosphatases (DUSPs). We hypothesized that single-nucleotide p...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/s41390-019-0502-9
更新日期:2020-01-01 00:00:00
abstract::Although fetal asphyxia, i.e. hypoxemia, acidosis, and hypercapnia, increases plasma arginine vasopressin (AVP) greater than 40-fold, hypoxemia and metabolic acidosis occurring independently cause only 5-fold and 2-fold increases, respectively. To determine the effects of hypercapnia on AVP release, we examined the ef...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199110000-00014
更新日期:1991-10-01 00:00:00
abstract::Blue and white phototherapy was given to infant (and weanling) homozygous Gunn rats treated with different doses of riboflavin-5-phosphate (ribofl.5'p.) During the first hours after a flavin-injection, the effect of phototherapy with both types of fluorescent lamps was enhanced. With equal radiant power applied, the s...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-197912000-00001
更新日期:1979-12-01 00:00:00
abstract:BACKGROUND:Cardiovascular (CV) complications are the most significant cause of mortality in adults with Cushing disease (CD); little is known about CV risk factors in children with CD. Measurement of lipoprotein particles by nuclear magnetic resonance (NMR) spectroscopy is a novel technology to assess CV risk. The obje...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/s41390-019-0438-0
更新日期:2019-09-01 00:00:00
abstract::The head sizes of 38 patients, growth hormone (GH) deficient following craniospinal (n = 26) or cranial irradiation (n = 12), have been assessed before (n = 38) and on completion of GH therapy (n = 15) or at the end of a similar period of observation without GH (n = 7). These results were compared to the change in hea...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198710000-00007
更新日期:1987-10-01 00:00:00
abstract::Supine sleeping, recommended to prevent sudden infant death, should be started in the delivery hospital, but meets concern that neonates may exhibit more intermittent hypoxia in this position. We aimed to demonstrate that intermittent hypoxia is equally rare in supine and side position. We enrolled 609 term neonates i...
journal_title:Pediatric research
pub_type: 杂志文章,随机对照试验
doi:10.1203/PDR.0b013e31819e76b1
更新日期:2009-06-01 00:00:00
abstract:BACKGROUND:A growing body of evidence defines inflammation as a hallmark feature of disease pathogenesis of Duchenne muscular dystrophy. To tailor potential immune modulatory interventions, a better understanding of immune dysregulation in Duchenne muscular dystrophy is needed. We now asked whether dystrophin deficienc...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/s41390-019-0427-3
更新日期:2019-08-01 00:00:00
abstract:BACKGROUND:The epithelial-mesenchymal transition (EMT) has been implicated as a key mechanism in the pathogenesis of liver fibrosis. The miR-200 family has been shown to inhibit EMT. METHODS:Liver fibrosis levels were assessed with Masson's trichrome staining of liver samples obtained from biliary atresia (BA) patient...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2014.181
更新日期:2015-02-01 00:00:00
abstract::Most preterm infants breathe at birth, but need additional respiratory support due to immaturity of the lung and respiratory control mechanisms. To avoid lung injury, the focus of respiratory support has shifted from invasive towards non-invasive ventilation. However, applying effective non-invasive ventilation is dif...
journal_title:Pediatric research
pub_type: 杂志文章,评审
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abstract::There is great interest in the regenerative potential of the neural stem cells and progenitors that populate the germinal zones of the immature brain. Studies using animal models of pediatric brain injuries have provided a clearer understanding of the responses of these progenitors to injury. In this review, we have c...
journal_title:Pediatric research
pub_type: 杂志文章,评审
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更新日期:2018-01-01 00:00:00
abstract::To evaluate the response of the small intestinal mucosa to Saccharomyces boulardii (S.b.), a yeast widely used in some countries as an adjuvant drug with oral antimicrobial therapy, seven healthy adult volunteers were treated with high doses of lyophilized S.b. (250 mg four times per day) for 2 wk. A peroral jejunal s...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198602000-00020
更新日期:1986-02-01 00:00:00
abstract::In eight newborn lambs, the adaption of blood to acidosis was studied by sequential measurements of P50 in vitro (pH 7.4; 37 degrees C) and P50 in vivo (animal's pH and temperature, 39 degrees C) during the course of HCl-induced metabolic acidosis. The benefit of the change in both P50 on tissue oxygen tension was stu...
journal_title:Pediatric research
pub_type: 杂志文章
doi:
更新日期:1981-05-01 00:00:00
abstract::An association between chorioamnionitis and periventricular leukomalacia has been reported in human preterm infants. However, whether this link is causal has not been convincingly established, and the underlying molecular mechanisms remain unclear. The objective of this study was to establish a reproducible model of c...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-200006000-00009
更新日期:2000-06-01 00:00:00
abstract::Human Bifidobacterium sp strain bifidum (B. bifidum) was administered to BALB/c lactating mice (n = 58) and their litters (n = 327 pups) to evaluate the ingested strain's adherent properties and ability to inhibit murine rotavirus (MRV) infection. ELISA and anaerobic bacteriologic techniques were used to measure MRV s...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199406000-00014
更新日期:1994-06-01 00:00:00
abstract::Yesterday's immunodeficiencies emphasized the clinical and familial associations of the syndromes and date from the 1920s (ataxia-telangiectasia, chronic mucocutaneous candidiasis), the 1930s (Wiskott-Aldrich syndrome), skipping the 1940s, but blossoming in the 15-y period from 1950 to 1965. In this period, primary im...
journal_title:Pediatric research
pub_type: 历史文章,杂志文章,评审
doi:10.1203/00006450-199305001-00007
更新日期:1993-01-01 00:00:00
abstract::The purpose of this investigation was to determine whether maximal oxygen consumption (VO2max) differed between clinically obese black and white children and if a difference existed to determine whether it was related to hematological profiles and/or physical activity/inactivity levels. Twenty-three black and 21 white...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.pdr.0000176909.66057.a3
更新日期:2005-09-01 00:00:00
abstract::Beta cells are partially replaced in neonatal rodents after deletion with streptozotocin (STZ). Exposure of pregnant rats to a low protein (LP) diet impairs endocrine pancreas development in the offspring, leading to glucose intolerance in adulthood. Our objective was to determine whether protein restriction has a sim...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e3181e17c90
更新日期:2010-07-01 00:00:00
abstract:BACKGROUND:Short-bowel syndrome (SBS) is the most common cause of neonatal intestinal failure. Recovery requires intestinal adaptation, dependent on enteral nutrition (EN) and growth factors such as glucagon-like peptide-2 (GLP-2), which is secreted from L cells in the ileum. Neonatal SBS often results in loss of ileum...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2013.44
更新日期:2013-06-01 00:00:00
abstract::Innate immunity and urinary tract response play a central role in the development of urinary tract infection (UTI). Heat shock protein (HSP) 72 and Toll-like receptor (TLR) 4 are among the key elements of innate defence mechanisms. This study assesses the role of HSPA1B A(1267)G and TLR4 A(896)G polymorphisms using al...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/pdr.0b013e318030d1f4
更新日期:2007-03-01 00:00:00
abstract::Zidovudine (azidothymidine, AZT) is used in pregnancy to reduce mother to infant transmission of HIV. Understanding the disposition of AZT in the fetus is necessary to optimize therapeutic regimens directed toward the fetus. Recent studies in primates found similar steady-state levels of the glucuronide metabolite of ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199807000-00008
更新日期:1998-07-01 00:00:00