Abstract:
:In order to elucidate the molecular mechanisms of cypermethrin resistance in Culex pipiens pallens Coq, the susceptible strain (SS strain) and cypermethrin resistant strain (CR strain) of Cx. p. pallens were investigated in this paper. The cypermethrin resistance ratio of CR strain to SS strain was measured by biological assays method, the cDNA sequence of sodium channel was cloned and analyzed. Real-time quantitative RT-PCR was used to detect the expression levels of the detoxification-related genes across between CR strain and SS strain of Cx. p. pallens. Bioassays indicated that CR strain was 283.06 and 80.68-fold resistance to cypermethrin and permethrin as compared to the susceptible strain, respectively. The sequence variability analysis of sodium channel gene between SS strain and CR strain shows that 4 point mutations (R954Q, L1023F, S1775G and A1989E) appear on the amino acid sequence of sodium channel of CR strain. The transcriptional levels of CYP6Z10, CYP9M10, CPGSTd1 and CPGSTd2 in the resistant strain are significantly higher than it is in the susceptible. The transcripts of CYP4H34 and E4 esterase have no significant difference between the CR strain and SS strain. The results indicated that sodium channel mutations, combined with elevated levels of P450s and GSTs, are associated with cypermethrin resistance in CR strain.
journal_name
Pestic Biochem Physioljournal_title
Pesticide biochemistry and physiologyauthors
Xu W,Liu S,Zhang Y,Gao J,Yang M,Liu X,Tao Ldoi
10.1016/j.pestbp.2017.01.008subject
Has Abstractpub_date
2017-10-01 00:00:00pages
77-82eissn
0048-3575issn
1095-9939pii
S0048-3575(17)30012-3journal_volume
142pub_type
杂志文章abstract::Widespread use of synthetic pesticides has resulted in the development of insecticide-resistant populations of pests and harmful effects on human health and the environment. There is a need to identify alternative pest management strategies to reduce our reliance on conventional chemical pesticides. In recent years th...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2015.08.003
更新日期:2016-01-01 00:00:00
abstract::Cantharidin (CTD) is a natural toxin with effective toxicity to lepidopteran pests. Nevertheless, little information is available on whether pests develop resistance to CTD. After being exposed to CTD (50 mg/L to 90 mg/L) or 10 generations, the resistance ratio of laboratory selected cantharidin-resistant Mythimna sep...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2020.104769
更新日期:2021-02-01 00:00:00
abstract::The beet armyworm, Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) is a highly polyphagous pest which causes considerable economic losses to cotton and many vegetable crops. Tannins are among the most important secondary metabolites in cotton plants. We show that tannic acid enhances the toxic effect of chlorantra...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2019.01.012
更新日期:2019-03-01 00:00:00
abstract::Although insecticide resistance is a widespread problem for most insect pests, frequently the assessment of resistance occurs over a limited geographic range. Herein, we report the first widespread survey of insecticide resistance in the USA ever undertaken for the house fly, Musca domestica, a major pest in animal pr...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2013.10.006
更新日期:2013-11-01 00:00:00
abstract::Galectins are a family of β-galactoside binding proteins, and insect galectins play a role in immune responses and may also affect Cry toxin activity. In this study, we aimed to further understand the function and molecular mechanism of Aedes aegypti galectin-6 in modulation of Cry11Aa toxicity. A. aegypti galectin-6 ...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2019.09.010
更新日期:2020-01-01 00:00:00
abstract::The present study focused on the toxicity of the aphid anti-feedant flonicamid and its main metabolite, 4-trifluoromethylnicotinamide (TFNA-AM) to Aedes aegypti and Anopheles gambiae mosquitoes. The compounds were toxic to both species via topical application, resulting in un-coordinated locomotion and leg splaying, w...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2018.06.004
更新日期:2018-10-01 00:00:00
abstract::The comparative efficacy and mechanism of action of the herbicide dymuron and its two optically active monomethyl analogues, (R)-1-(alpha-methylbenzyl)-3-(p-tolylurea) (R-MBTU) and S-MBTU, as safeners of rice against injury from bensulfuron-methyl were investigated. Bioassays using etiolated seedlings of rice (Oryza s...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1006/pest.1996.0035
更新日期:1996-05-01 00:00:00
abstract::Paraquat (PQ) as a herbicide and an environmental pollutant with increasing importance due to its toxicity to humans and animals. This study aimed to evaluate the protective and antioxidant activity of quercetin loaded Nanostructured Lipid Carriers (QNLC) against toxicity induced by PQ. Blood lymphocytes were prepared...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2020.104586
更新日期:2020-07-01 00:00:00
abstract::Xanthomonas oryzae pv. oryzae (Xoo) is the casual agent of bacterial blight, which is one of the most serious diseases of rice. The antibiotic phenazine-1-carboxylic acid (PCA), which is primarily produced by Pseudomonas spp., was found and previously reported very effective against Xoo. However, the biological effect...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2014.10.006
更新日期:2015-01-01 00:00:00
abstract::The use of transgenic crops that induce silencing of essential genes using double-stranded RNA (dsRNA) through RNA interference (RNAi) in western corn rootworm, Diabrotica virgifera virgifera, is likely to be an important component of new technologies for the control of this important corn pest. Previous studies have ...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2018.04.004
更新日期:2018-06-01 00:00:00
abstract::Tetrahydrophthalimidobenzoates are a class of protoporphyrinogen oxidase herbicides acting on the protoporphyrinogen oxidase enzyme. After the discovery of compound 1, a series of novel tetrahydrophthalimidobenzoate derivatives were designed and synthesized, and some synthesized compounds exhibited good herbicidal act...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2017.04.007
更新日期:2017-06-01 00:00:00
abstract::1-Deoxynojirimycin (DNJ) is a natural d-glucose analogue from mulberry with promising physiological activity in vivo. Up to the present, the antidiabetic effects of DNJ on lowering blood sugar and accelerating lipid metabolism in mammals were broadly reported, but the specific character of DNJ against insects was vast...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2018.08.009
更新日期:2018-11-01 00:00:00
abstract::Unlike chemical pesticides, antiviral plants are biodegradable, replenishable and safe. In this study, 14 sesquiterpene compounds from Tithonia diversifolia were tested for their activities against Tobacco mosaic virus (TMV) using the half-leaf method. Tagitinin C (Ses-2) and 1β-methoxydiversifolin-3-0-methyl ether (S...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2017.05.009
更新日期:2017-08-01 00:00:00
abstract::Inheritance of resistance to phosphine fumigant was investigated in three field-collected strains of rusty grain beetle, Cryptolestes ferrugineus, Susceptible (S-strain), Weakly Resistant (Weak-R) and Strongly Resistant (Strong-R). The strains were purified for susceptibility, weak resistance and strong resistance to ...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2015.09.008
更新日期:2016-02-01 00:00:00
abstract::Deltamethrin belongs to the class of synthetic pyrethroids, which are being widely used as insecticides in agricultural practices. Voltage-gated sodium channels (VGSCs) are the primary targets of these chemicals for toxicity to insects. Caenorhabditis elegans (C. elegans) does not have VGSCs but is susceptible to delt...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2017.03.005
更新日期:2017-05-01 00:00:00
abstract::Helicoverpa armigera is one of the major crop pests and is less amenable to current pest control approaches. RNA interference (RNAi) is emerging as a potent arsenal for the insect pest control over current methods. Here, we examined the effect on growth and development in H. armigera by targeting various enzymes/prote...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2016.03.006
更新日期:2016-10-01 00:00:00
abstract::The mirid bug is frequently controlled by the application of organophosphorus insecticides in the transgenic Bt cotton field of China. A topical bioassay method was performed to evaluate the toxicities of chlorpyrifos and malathion towards field-collected Chinese populations of Apolygus lucorum from transgenic Bt cott...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2016.04.008
更新日期:2016-09-01 00:00:00
abstract::Pyrethrum extract (PY) is a natural insecticide that is extensively used across the world, and its insecticidal activity is attributed to the presence of six active esters known as pyrethrins. PY targets the nervous systems of insects by delaying the closure of voltage-gated sodium ion channels in the nerve cells. How...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2016.09.003
更新日期:2017-04-01 00:00:00
abstract::We previously reported emergence of super synthetic pyrethroid (SP) resistant Rhipicephalus (Boophilus) decoloratus ticks in Uganda. This study investigated the genetic basis of phenotypic resistance against SP in R. (B.) decoloratus and sought to identify novel diagnostic mutations for rapid detection of SP resistanc...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2017.07.009
更新日期:2017-11-01 00:00:00
abstract::The development of plant protection product (PPPs)-resistant populations of plant pathogens, pests, and weeds, represents a major challenge that the crop protection sector is facing. Focusing on plant pathogenic fungi, the increased efflux of the active ingredients (a.i.) from the cytoplasm is highly correlated to ele...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2019.09.013
更新日期:2020-05-01 00:00:00
abstract::The incidence of mosquito-borne disease poses a significant threat to human and animal health throughout the world, with effective chemical control interventions limited by widespread insecticide resistance. Recent evidence suggests that gut bacteria of mosquitoes, known to be essential in nutritional homeostasis and ...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2019.07.016
更新日期:2019-11-01 00:00:00
abstract::Spirotetramat were now widely used for control insecticides resistant aphids since 2011 in China. In order to elucidate the possible resistance mechanism, a laboratory selected resistant strain (SR) of cotton aphid was established with a 578.93-fold and 14.91-fold resistance ratio to spirotetramat for adult aphids and...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2015.02.002
更新日期:2015-03-01 00:00:00
abstract::Meta-diamides [3-benzamido-N-(4-(perfluoropropan-2-yl)phenyl)benzamides] are a distinct class of RDL GABA receptor noncompetitive antagonists showing high insecticidal activity against Spodoptera litura. The mode of action of the meta-diamides was demonstrated to be distinct from that of conventional noncompetitive an...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章,评审
doi:10.1016/j.pestbp.2014.09.010
更新日期:2015-06-01 00:00:00
abstract::One of the most famous and commonly used compounds from organophosphate (OP) family is chlorpyrifos (CP) which is widespreadly used as a powerful insecticide. Previous studies have shown that OPs induce oxidative stress, inflammation and apoptosis by generating the free radicals. The protective effects of three member...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2015.10.005
更新日期:2016-03-01 00:00:00
abstract::Mature and healthy male lesser bandicoot rats, Bandicota bengalensis (n = 40) were fed on bait (mixture of cracked wheat and powdered sugar in 98:2) containing different concentrations of triptolide (0, 0.15, 0.20 and 0.25% w/w) for 15 days in two-choice trials. Results revealed no significant effect of triptolide tre...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2014.09.012
更新日期:2014-11-01 00:00:00
abstract::Rhinella icterica is a poisonous toad whose toxic secretion has never been studied against entomotoxic potential. Sublethal doses of Rhinella icterica toxic secretion (RITS) were assayed in Nauphoeta cinerea cockroaches, in order to understand the physiological and behavioral parameters, over the insect central and pe...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2018.04.016
更新日期:2018-06-01 00:00:00
abstract::Cucumber mosaic virus (CMV) is a plant virus with one of the largest host ranges, the widest distribution, and economic importance, and ningnanmycin (NNM) is a commercial antiviral agent. Studies have shown that NNM induces and promotes pathogenesis-related proteins in tobacco mosaic virus-inoculated tobacco. In the p...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2019.02.012
更新日期:2019-05-01 00:00:00
abstract::In order to find a novel type of fluorescent phloem-mobile insecticides, a facile one-pot solvothermal strategy via fipronil addition-elimination substitution reaction with the corresponding acyl chloride derivatives has been employed to construct series of phenylpyrazole amide derivatives (PAs) fused olefin moieties ...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2019.10.003
更新日期:2020-02-01 00:00:00
abstract::Asia minor bluegrass (Polypogon fugax) is a common annual grass weed of winter crops distributed across China. We conducted a study on the resistance level and the mechanism of resistance to ACCase-inhibiting herbicides in a P. fugax population from China. Whole-plant dose-response experiments in greenhouse showed tha...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2013.11.001
更新日期:2014-01-01 00:00:00
abstract::Sodium channel blocker insecticides (SCBIs) are a relatively new class of insecticides, with a mechanism of action different from those of other classes of insecticides that target voltage-gated sodium channels. These compounds have no effect at hyperpolarized membrane potentials, but cause a voltage-dependent, nearly...
journal_title:Pesticide biochemistry and physiology
pub_type: 杂志文章
doi:10.1016/j.pestbp.2009.09.001
更新日期:2010-06-01 00:00:00