Abstract：
:The hypothesis that the recent rapid evolution of primate cytochromes c, which primarily involves residues in the least stable Ω-loop (Ω-loop C, residues 40-57), stabilizes the heme crevice of cytochrome c relative to other mammals, is tested. To accomplish this goal, we have compared the properties of human and spider monkey cytochrome c and a set of four variants produced in the process of converting human cytochrome c into spider monkey cytochrome c. The global stability of all variants has been measured by guanidine hydrochloride denaturation. The stability of the heme crevice has been assessed with the alkaline conformational transition. Structural insight into the effects of the five amino acid substitutions needed to convert human cytochrome c into spider monkey cytochrome c is provided by a 1.15Å resolution structure of spider monkey cytochrome c. The global stability for all variants is near 9.0kcal/mol at 25°C and pH7, which is higher than that observed for other mammalian cytochromes c. The heme crevice stability is more sensitive to the substitutions required to produce spider monkey cytochrome c with decreases of up to 0.5 units in the apparent pKa of the alkaline conformational transition relative to human cytochrome c. The structure of spider monkey cytochrome c indicates that the Y46F substitution destabilizes the heme crevice by disrupting an extensive hydrogen bond network that connects three surface loops including Ω-loop D (residues 70-85), which contains the Met80 heme ligand.
journal_name
J Inorg Biochemjournal_title
Journal of inorganic biochemistryauthors
Goldes ME,Jeakins-Cooley ME,McClelland LJ,Mou TC,Bowler BEdoi
10.1016/j.jinorgbio.2015.12.025subject
Has Abstractpub_date
2016-05-01 00:00:00pages
62-69eissn
0162-0134issn
1873-3344pii
S0162-0134(15)30156-2journal_volume
158pub_type
杂志文章abstract：:Industrial applications and environmental problems involving the aqueous chemistry of aluminium require an understanding of the speciation of this metal ion at a wide range of concentrations. The formation of polynuclear species is of special interest due to the complexity of the hydrolysis mechanisms and the diversit...
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journal_title：Journal of inorganic biochemistry
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
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更新日期：2020-05-01 00:00:00
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
doi：10.1016/0162-0134(90)84002-7
更新日期：1990-07-01 00:00:00
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
doi：10.1016/j.jinorgbio.2004.11.023
更新日期：2005-02-01 00:00:00
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
doi：10.1016/j.jinorgbio.2006.09.017
更新日期：2006-12-01 00:00:00
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
doi：10.1016/j.jinorgbio.2006.02.009
更新日期：2006-05-01 00:00:00
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pub_type： 杂志文章
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章,评审
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更新日期：1992-08-15 00:00:00
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
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更新日期：2012-02-01 00:00:00
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
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更新日期：2019-06-01 00:00:00
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
doi：10.1016/0162-0134(88)80005-9
更新日期：1988-08-01 00:00:00
abstract：:Protein tyrosine nitration, protein oxidation and lipid peroxidation are nitrative/oxidative modification of protein and lipids. In this paper, a BSA (bovine serum albumin)-lecithin liposome system was used to study the nature of different forms of iron, including methemoglobin, hemin and ferric citrate, in catalyzing...
journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
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更新日期：2009-05-01 00:00:00
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
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更新日期：2002-01-01 00:00:00
abstract：:Four water soluble complexes of the type [M(bpy)(a-x)]NO3, where M is Pd(II) or Pt(II), bpy is 2,2-bipyridine, and a-x is anion of 2-pyridinecarboxylic acid or 2-pyrazinecarboxylic acid, have been found to bind calf thymus DNA, possibly through hydrogen binding. [M(bpy)(2-py)]NO3 complexes (2-py is an anion of 2-pyrid...
journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
doi：10.1016/0162-0134(92)80054-y
更新日期：1992-10-01 00:00:00
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
doi：10.1016/0162-0134(90)80041-u
更新日期：1990-09-01 00:00:00
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journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
doi：10.1016/j.jinorgbio.2013.10.023
更新日期：2014-02-01 00:00:00
abstract：:EPR-based potentiometric titrations are a well-established method for determining the reduction potentials of cofactors in large and complex proteins with at least one EPR-active state. However, such titrations require large amounts of protein. Here, we report a new method that requires an order of magnitude less prot...
journal_title：Journal of inorganic biochemistry
pub_type： 杂志文章
doi：10.1016/j.jinorgbio.2016.04.025
更新日期：2016-09-01 00:00:00
