Abstract:
:Colony forming cells (CFC) with high proliferative potential have been detected in nutrient agar cultures of human bone marrow cells containing recombinant human interleukin-3 (IL-3) and granulocyte macrophage colony stimulating factor (GM-CSF). These CFC were detected by the formation of large colonies with diameters greater than 0.5 mm and containing approximately 50,000 cells after 28 days incubation. The incidence of these CFC was only two in 100,000 normal bone marrow cells; however, bone marrow from patients treated with 5-fluorouracil contained up to sevenfold higher numbers of these CFC. The characteristics of these CFC, multifactor-responsive progenitors with high proliferative potential, requiring a prolonged growth period in culture and showing a relative preservation in marrow from individuals pretreated with 5-fluorouracil, are consistent with a human cell type equivalent to the primitive murine progenitor termed HPP-CFC.
journal_name
Bloodjournal_title
Bloodauthors
McNiece IK,Stewart FM,Deacon DM,Temeles DS,Zsebo KM,Clark SC,Quesenberry PJsubject
Has Abstractpub_date
1989-08-01 00:00:00pages
609-12issue
2eissn
0006-4971issn
1528-0020journal_volume
74pub_type
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