Matrix-assisted laser desorption ionization-time of flight: a promising alternative method of identifying the major coagulase-negative Staphylococci species.

Abstract:

INTRODUCTION.:This study aimed to evaluate different methods for differentiation of species of coagulase-negative staphylococci (CoNS) that caused infections in hospitalized immunocompromised patients. METHODS.:A total of 134 CoNS strains were characterized using four different methods. RESULTS. :The results of matrix assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) analysis were in complete agreement with those of tuf gene sequencing (kappa index = 1.00). The kappa index of Vitek 2® Compact analysis was 0.85 (very good) and that of the conventional method was 0.63 (moderate). CONCLUSIONS:. MALDI-TOF MS provided rapid and accurate results for the identification of CoNS (134; 100%).

journal_name

Rev Soc Bras Med Trop

authors

Trevisoli LE,Bail L,Rodrigues LS,Conte D,Palmeiro JK,Dalla-Costa LM

doi

10.1590/0037-8682-0026-2017

subject

Has Abstract

pub_date

2018-01-01 00:00:00

pages

85-87

issue

1

eissn

0037-8682

issn

1678-9849

pii

S0037-86822018000100085

journal_volume

51

pub_type

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