Abstract:
:Precise replacement of an existing allele in commercial cultivars with an elite allele is a major goal in crop breeding. A single nucleotide polymorphism in the NRT1.1B gene between japonica and indica rice is responsible for the improved nitrogen use efficiency in indica rice. Herein, we precisely replaced the japonica NRT1.1B allele with the indica allele, in just one generation, using CRISPR/Cas9 gene-editing technology. No additional selective pressure was needed to enrich the precise replacement events. This work demonstrates the feasibility of replacing any genes with elite alleles within one generation, greatly expanding our ability to improve agriculturally important traits.
journal_name
J Integr Plant Bioljournal_title
Journal of integrative plant biologyauthors
Li J,Zhang X,Sun Y,Zhang J,Du W,Guo X,Li S,Zhao Y,Xia Ldoi
10.1111/jipb.12650subject
Has Abstractpub_date
2018-07-01 00:00:00pages
536-540issue
7eissn
1672-9072issn
1744-7909journal_volume
60pub_type
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journal_title:Journal of integrative plant biology
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