Fast pesticide pre-screening in marine environment using a green microalgae-based optical bioassay.

Abstract:

:The present study evaluates an optical bioassay based on green photosynthetic microalgae as a promising alternative for monitoring of relevant seawater pollutants. Photosystem II fluorescence parameters from several microalgae species were examined in the presence of three common marine pesticides that act as photosynthesis inhibitors. The three pollutants were detected within 10 min in concentrations between ng/L-μg/L. The different algae species showed slightly diverse pesticide sensitivities, being Chlorella mirabilis the most sensitive one. Potential interferences due to oil-spill pollutants were discarded. The lipid content was characterized to identify microorganisms with suitable mechanisms that could facilitate stress acclimatization. C. mirabilis presented elevated content of unsaturated lipids, showing a promising potential for biosensing in saline stress conditions. The optimized microalgae-based bioassay was preliminarily incorporated into a marine buoy for autonomous pre-screening of pesticides in coastal areas, demonstrating its suitability for real-time monitoring of marine water and quantitative evaluation of total biotoxicity.

journal_name

Mar Pollut Bull

authors

Moro L,Pezzotti G,Turemis M,Sanchís J,Farré M,Denaro R,Giacobbe MG,Crisafi F,Giardi MT

doi

10.1016/j.marpolbul.2018.02.036

subject

Has Abstract

pub_date

2018-04-01 00:00:00

pages

212-221

issue

1

eissn

0025-326X

issn

1879-3363

pii

S0025-326X(18)30124-3

journal_volume

129

pub_type

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