Multiplex gene editing in rice with simplified CRISPR-Cpf1 and CRISPR-Cas9 systems.

Abstract:

:We developed simplified single transcriptional unit (SSTU) CRISPR systems for multiplex gene editing in rice using FnCpf1, LbCpf1 or Cas9, in which the nuclease and its crRNA array are co-expressed from a single Pol II promoter, without any additional processing machinery. Our SSTU systems are easy to construct and effective in mediating multiplex genome editing.

journal_name

J Integr Plant Biol

authors

Wang M,Mao Y,Lu Y,Wang Z,Tao X,Zhu JK

doi

10.1111/jipb.12667

subject

Has Abstract

pub_date

2018-08-01 00:00:00

pages

626-631

issue

8

eissn

1672-9072

issn

1744-7909

journal_volume

60

pub_type

信件
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