Abstract:
:We developed simplified single transcriptional unit (SSTU) CRISPR systems for multiplex gene editing in rice using FnCpf1, LbCpf1 or Cas9, in which the nuclease and its crRNA array are co-expressed from a single Pol II promoter, without any additional processing machinery. Our SSTU systems are easy to construct and effective in mediating multiplex genome editing.
journal_name
J Integr Plant Bioljournal_title
Journal of integrative plant biologyauthors
Wang M,Mao Y,Lu Y,Wang Z,Tao X,Zhu JKdoi
10.1111/jipb.12667subject
Has Abstractpub_date
2018-08-01 00:00:00pages
626-631issue
8eissn
1672-9072issn
1744-7909journal_volume
60pub_type
信件abstract::Pollen development is a critical step in plant development that is needed for successful breeding and seed formation. Manipulation of male fertility has proved a useful trait for hybrid breeding and increased crop yield. However, although there is a good understanding developing of the molecular mechanisms of anther a...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章,评审
doi:10.1111/jipb.12425
更新日期:2015-11-01 00:00:00
abstract::In C3 plants, photorespiration is an energy-expensive process, including the oxygenation of ribulose-1,5-bisphosphate (RuBP) by ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) and the ensuing multi-organellar photorespiratory pathway required to recycle the toxic byproducts and recapture a portion of the fix...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章,评审
doi:10.1111/jipb.12709
更新日期:2018-12-01 00:00:00
abstract::More than a billion people suffer from iron or zinc deficiencies globally. Rice (Oryza sativa L.) iron and zinc biofortification; i.e., intrinsic iron and zinc enrichment of rice grains, is considered the most effective way to tackle these deficiencies. However, rice iron biofortification, by means of conventional bre...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章,评审
doi:10.1111/jipb.12751
更新日期:2018-12-01 00:00:00
abstract::Two new neo-clerodane diterpenoids, 6,7-dibenzoyloxybarbatin C (1, named barbatin D) and 6-(2-acetoxy-3-methylbutanoloxy)-7-(2-carbonyl-3-methylbutanoyloxy) barbatin C (2, named barbatin E) were isolated from the whole plant of Scutellaria barbata D. Don. Their structures were elucidated by spectroscopic methods inclu...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/j.1744-7909.2008.00672.x
更新日期:2008-06-01 00:00:00
abstract::Variations in the trnK region of chloroplast DNA were investigated in the present study using polymerase chain reaction-restriction fragment length polymorphism to detect the genetic structure and to infer the possible glacial refugia of Ginkgo biloba L. in China. In total, 220 individuals from 12 populations in China...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/j.1744-7909.2007.00375.x
更新日期:2008-03-01 00:00:00
abstract::Nitrogen-limited conditions are considered to be a prerequisite for legume-rhizobial symbiosis, but the effects of nitrate-rich conditions on symbiotic status remain poorly understood. We addressed this issue by examining rhizobial (Rhizobim tropici) and arbusclar mycorrhizal (Glomus intraradices) symbiosis in Phaseol...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/jipb.12156
更新日期:2014-03-01 00:00:00
abstract::With the enhancement of copper (Cu) stress, the germination percentage of wheat seeds decreased gradually. Pretreatment with sodium hydrosulfide (NaHS), hydrogen sulfide (H(2)S) donor alleviated the inhibitory effect of Cu stress in a dose-dependent manner; whereas little visible symptom was observed in germinating se...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/j.1744-7909.2008.00769.x
更新日期:2008-12-01 00:00:00
abstract::Protein dephosphorylation mediated by protein phosphatases plays a major role in signal transduction of plant responses to environmental stresses. In this study, two putative protein phosphatases, PvPS2:1 and PvPS2:2 were identified and characterized in bean (Phaseolus vulgaris). The two PvPS2 members were found to be...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/j.1744-7909.2012.01126.x
更新日期:2012-06-01 00:00:00
abstract::The small phenolic compound salicylic acid (SA) plays an important regulatory role in multiple physiological processes including plant immune response. Significant progress has been made during the past two decades in understanding the SA-mediated defense signaling network. Characterization of a number of genes functi...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章,评审
doi:10.1111/j.1744-7909.2011.01043.x
更新日期:2011-06-01 00:00:00
abstract::The main objective of the present study was to identify simple sequence repeat (SSR) markers associated with the amino acid content of rice (Oryza sativa L.). SSR markers were selected by prescreening for the relationship to amino acid content. Eighty-four rice landrace accessions from Korea were evaluated for 16 kind...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/j.1744-7909.2009.00883.x
更新日期:2009-12-01 00:00:00
abstract::During daylight, plants produce excess photosynthates, including sucrose, which is temporarily stored in the vacuole. At night, plants remobilize sucrose to sustain metabolism and growth. Based on homology to other sucrose transporter (SUT) proteins, we hypothesized the maize (Zea mays) SUCROSE TRANSPORTER2 (ZmSUT2) p...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/jipb.12527
更新日期:2017-06-01 00:00:00
abstract::Fusarium head blight (FHB) caused by Fusarium graminearum Schwabe (teleomorph Gibberella zeae (Schw.) Perch) results in large yield losses in annual global wheat production. Although studies have identified a number of wheat FHB resistance genes, a deeper understanding of the mechanisms underlying host plant resistanc...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/jipb.12992
更新日期:2020-07-17 00:00:00
abstract::A narrow leaf mutant was isolated from transgenic rice (Oryza sativa L.) lines carrying a T-DNA insertion. The mutant is characterized by narrow leaves during its whole growth period, and was named nal9 (narrow leaf 9). The mutant also has other phenotypes, such as light green leaves at the seedling stage, reduced pla...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/jipb.12098
更新日期:2013-11-01 00:00:00
abstract::The amino-terminal residue of a protein (or amino-terminus of a peptide following protease cleavage) can be an important determinant of its stability, through the Ubiquitin Proteasome System associated N-degron pathways. Plants contain a unique combination of N-degron pathways (previously called the N-end rule pathway...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章,评审
doi:10.1111/jipb.12882
更新日期:2020-01-01 00:00:00
abstract::The relationship between structural features of various vegetative organs and triterpenoid saponin accumulation in Achyranthus bidentata Blume was investigated using anatomy, histochemistry and phytochemistry. The results showed that the primary and secondary structures of roots, and the structures of stems and leaves...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/j.1744-7909.2008.00764.x
更新日期:2009-02-01 00:00:00
abstract::Domain of unknown function 1644 (DUF1644) is a highly conserved amino acid sequence motif present only in plants. Analysis of expression data of the family of DUF1644-containing genes indicated that they may regulate responses to abiotic stress in rice. Here we present our discovery of the role of OsSIDP366, a member ...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/jipb.12376
更新日期:2016-05-01 00:00:00
abstract::The eukaryotic actin cytoskeleton is required for numerous cellular processes, including cell shape, development and movement, gene expression and signal transduction, and response to biotic and abiotic stress. In recent years, research in both plants and animal systems have described a function for actin as the ideal...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章,评审
doi:10.1111/jipb.12445
更新日期:2016-04-01 00:00:00
abstract::The frequency and distribution of the major vernalization requirement genes and their effects on growth habits were studied. Of the 551 bread wheat genotypes tested, seven allelic combinations of the three Vrn-1 genes were found to be responsible for the spring habit, three for the facultative habit and one for the wi...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/j.1744-7909.2009.00812.x
更新日期:2009-04-01 00:00:00
abstract::Wheat grown in Mn-deficient soil has been widely observed to produce much reduced yields. Breeding for Mn-efficient wheat genotypes adapted to Mn-deficient soils would represent a long-term solution for wheat agronomy. To characterize the physiological basis of Mn efficiency in wheat genotypes would facilitate the bre...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/j.1744-7909.2007.00631.x
更新日期:2008-04-01 00:00:00
abstract::In rice, one detrimental factor influencing single panicle yield is the frequent occurrence of panicle apical abortion (PAA) under unfavorable climatic conditions. Until now, no detailed genetic information has been available to avoid PAA in rice breeding. Here, we show that the occurrence of PAA is associated with th...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/j.1744-7909.2011.01055.x
更新日期:2011-09-01 00:00:00
abstract::A potential mechanism to enhance utilization of sparingly soluble forms of phosphorus (P) is the root secretion of malate, which is mainly mediated by the ALMT gene family in plants. In this study, a total of 34 GmALMT genes were identified in the soybean genome. Expression patterns diverged considerably among GmALMTs...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/jipb.12604
更新日期:2018-03-01 00:00:00
abstract::The thermosensitive genic male sterile 5 (tms5) mutation causes thermosensitive genic male sterility in rice (Oryza sativa) through loss of RNase ZS1 function, which influences ubiquitin fusion ribosomal protein L40 (UbL40 ) messenger RNA levels during male development. Here, we used ATAC-seq, combined with analysis o...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/jipb.12871
更新日期:2020-07-01 00:00:00
abstract::Class III secretable plant peroxidases occur as a large family of genes in plants with many functions and probable redundancy. In this review we are concentrating on the evidence we have on the catalysis of lignin polymerization by class III plant peroxidases present in the apoplastic space in the xylem of trees. Some...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章,评审
doi:10.1111/j.1744-7909.2010.00928.x
更新日期:2010-02-01 00:00:00
abstract::We have identified hpm91, a Chlamydomonas mutant lacking Proton Gradient Regulation5 (PGR5) capable of producing hydrogen (H2 ) for 25 days with more than 30-fold yield increase compared to wild type. Thus, hpm91 displays a higher capacity of H2 production than a previously characterized pgr5 mutant. Physiological and...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/jipb.12502
更新日期:2016-12-01 00:00:00
abstract::Vitex rotundifolia L. is an important plant species used in traditional Chinese medicine. For its efficient use and conservation, genetic diversity and clonal variation of V. rotundifolia populations in China were investigated using inter-simple sequence repeat markers. Fourteen natural populations were included to es...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/j.1744-7909.2008.00635.x
更新日期:2008-09-01 00:00:00
abstract::Association mapping is a powerful approach for exploring the molecular basis of phenotypic variations in plants. A peanut (Arachis hypogaea L.) mini-core collection in China comprising 298 accessions was genotyped using 109 simple sequence repeat (SSR) markers, which identified 554 SSR alleles and phenotyped for 15 ag...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/jipb.12132
更新日期:2014-02-01 00:00:00
abstract::Nuclear migration and positioning are crucial for the morphogenesis of plant cells. We addressed the potential role of nuclear positioning for polarity induction using an experimental system based on regenerating protoplasts, where the induction of a cell axis de novo can be followed by quantification of specific rege...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/jipb.12469
更新日期:2016-09-01 00:00:00
abstract::High-quality and disease-resistant male sterile lines have great potential for applications in hybrid rice breeding. We introduced specific mutations into the TMS5, Pi21, and Xa13 genes in Pinzhan intermediate breeding material using the CRISPR/Cas9 multiplex genome editing system. We found that the transgene-free hom...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/jipb.12774
更新日期:2019-12-01 00:00:00
abstract::Pentatricopeptide repeat (PPR) proteins, characterized by tandem arrays of a 35 amino acid motif, have been suggested to play central and broad roles in modulating the expression of organelle genes in plants. However, the molecular mechanisms of most rice PPR genes remains unclear. In this paper, we isolated and chara...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章
doi:10.1111/jipb.12138
更新日期:2014-04-01 00:00:00
abstract::The advent of clustered regularly interspaced short palindromic repeat (CRISPR) has had a profound impact on plant biology, and crop improvement. In this review, we summarize the state-of-the-art development of CRISPR technologies and their applications in plants, from the initial introduction of random small indel (i...
journal_title:Journal of integrative plant biology
pub_type: 杂志文章,评审
doi:10.1111/jipb.13063
更新日期:2021-01-01 00:00:00