InhA1-Mediated Cleavage of the Metalloprotease NprA Allows Bacillus cereus to Escape From Macrophages.

Abstract:

:Bacillus cereus is a Gram-positive spore-forming bacterium causing food poisoning and serious opportunistic infections. These infections are characterized by bacterial accumulation in the host despite the induction of inflammation. To circumvent inflammation, bacteria must resist the bactericidal activity of professional phagocytes, which constitute a first line of host defense against pathogens. Interactions between phagocytic cells and B. cereus are still poorly characterized and the mechanism of resistance to the host immune system is not known yet. We have previously shown that the spores are phagocytosed by macrophages but survive and escape from these cells. The metalloprotease InhA1 is a key effector involved in these processes. inhA1-deficient spores are retained intracellularly, in contrast to the wild type strain spores. NprA is also a B. cereus metalloprotease able to cleave tissue components such as fibronectin, laminin, and collagen. Here, we show that NprA, concomitantly secreted with InhA1 in the B. cereus secretome, is essential to promote bacterial escape from macrophages. We show that InhA1 cleaves NprA at specific sites. This cleavage allows liberation of the mature form of the NprA protein in the supernatant of the wild type strain. This mature form of NprA is actually the principal effector allowing bacterial escape from host macrophages.

journal_name

Front Microbiol

authors

Haydar A,Tran SL,Guillemet E,Darrigo C,Perchat S,Lereclus D,Coquet L,Jouenne T,Ramarao N

doi

10.3389/fmicb.2018.01063

subject

Has Abstract

pub_date

2018-05-23 00:00:00

pages

1063

issn

1664-302X

journal_volume

9

pub_type

杂志文章
  • The Impact of ackA, pta, and ackA-pta Mutations on Growth, Gene Expression and Protein Acetylation in Escherichia coli K-12.

    abstract::Acetate is a characteristic by-product of Escherichia coli K-12 growing in batch cultures with glucose, both under aerobic as well as anaerobic conditions. While the reason underlying aerobic acetate production is still under discussion, during anaerobic growth acetate production is important for ATP generation by sub...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00233

    authors: Schütze A,Benndorf D,Püttker S,Kohrs F,Bettenbrock K

    更新日期:2020-02-21 00:00:00

  • Gastrointestinal Tract As Entry Route for Hantavirus Infection.

    abstract::Background: Hantaviruses are zoonotic agents that cause hemorrhagic fevers and are thought to be transmitted to humans by exposure to aerosolized excreta of infected rodents. Puumala virus (PUUV) is the predominant endemic hantavirus in Europe. A large proportion of PUUV-infected patients suffer from gastrointestinal ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01721

    authors: Witkowski PT,Perley CC,Brocato RL,Hooper JW,Jürgensen C,Schulzke JD,Krüger DH,Bücker R

    更新日期:2017-09-08 00:00:00

  • Differential Responses by Human Macrophages to Infection With Mycobacterium tuberculosis and Non-tuberculous Mycobacteria.

    abstract::Mycobacterium tuberculosis (MTB) and non-tuberculous mycobacteria (NTM) are formidable causes of lung diseases throughout the world. While MTB is considered to be more virulent than NTM, host factors also play a key role in disease development. To elucidate whether there are differential immune responses to various my...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00116

    authors: Feng Z,Bai X,Wang T,Garcia C,Bai A,Li L,Honda JR,Nie X,Chan ED

    更新日期:2020-02-07 00:00:00

  • Potential drivers of microbial community structure and function in Arctic spring snow.

    abstract::The Arctic seasonal snowpack can extend at times over a third of the Earth's land surface. This chemically dynamic environment interacts constantly with different environmental compartments such as atmosphere, soil and meltwater, and thus, strongly influences the entire biosphere. However, the microbial community asso...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00413

    authors: Maccario L,Vogel TM,Larose C

    更新日期:2014-08-07 00:00:00

  • Fecal Microbiota Characterization of Seychelles Giant Tortoises (Aldabrachelys gigantea) Living in Both Wild and Controlled Environments.

    abstract::A microbiome is defined as a complex collection of microorganisms and their genetic material. Studies regarding gut microbiomes of different animals have provided ecological and evolutionary information showing a strong link between health and disease. Very few studies have compared the gut microbiota of animals house...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.569249

    authors: Sandri C,Correa F,Spiezio C,Trevisi P,Luise D,Modesto M,Remy S,Muzungaile MM,Checcucci A,Zaborra CA,Mattarelli P

    更新日期:2020-10-20 00:00:00

  • Identification of the Tolfenamic Acid Binding Pocket in PrbP from Liberibacter asiaticus.

    abstract::In Liberibacter asiaticus, PrbP is an important transcriptional accessory protein that was found to regulate gene expression through interactions with the RNA polymerase β-subunit and a specific sequence on the promoter region. It was found that inactivation of PrbP, using the inhibitor tolfenamic acid, resulted in a ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01591

    authors: Pan L,Gardner CL,Pagliai FA,Gonzalez CF,Lorca GL

    更新日期:2017-08-23 00:00:00

  • Bacterial Community Composition in the Sea Surface Microlayer Off the Peruvian Coast.

    abstract::The sea surface microlayer (SML) is located at the air-sea interface, with microorganisms and organic matter in the SML influencing air-sea exchange processes. Yet understanding of the SML bacterial (bacterioneuston) community composition and assembly remains limited. Availability of organic matter, UV radiation and w...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02699

    authors: Zäncker B,Cunliffe M,Engel A

    更新日期:2018-11-15 00:00:00

  • Protective Effector Cells of the Recombinant Asp f3 Anti-Aspergillosis Vaccine.

    abstract::An Aspergillus fumigatus vaccine based on recombinant Asp f3-protein has the potential to prevent aspergillosis in humans, a devastating fungal disease that is the prime obstacle to the success of hematopoietic cell transplantation. This vaccine protects cortisone acetate (CA)-immunosuppressed mice from invasive pulmo...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00299

    authors: Diaz-Arevalo D,Ito JI,Kalkum M

    更新日期:2012-08-22 00:00:00

  • Plasmid-Mediated Transmission of KPC-2 Carbapenemase in Enterobacteriaceae in Critically Ill Patients.

    abstract::Carbapenem-resistant Enterobacteriaceae (CRE) cause health care-associated infections worldwide, and they are of severe concern due to limited treatment options. We report an outbreak of KPC-2-producing CRE that was caused by horizontal transmission of a promiscuous plasmid across different genera of bacteria and hosp...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00276

    authors: Schweizer C,Bischoff P,Bender J,Kola A,Gastmeier P,Hummel M,Klefisch FR,Schoenrath F,Frühauf A,Pfeifer Y

    更新日期:2019-02-19 00:00:00

  • Identification, Functional Characterization, and Regulon Prediction of the Zinc Uptake Regulator (zur) of Bacillus anthracis - An Insight Into the Zinc Homeostasis of the Pathogen.

    abstract::Zinc has an abounding occurrence in the prokaryotes and plays paramount roles including catalytic, structural, and regulatory. Zinc uptake regulator (Zur), a Fur family transcriptional regulator, is connoted in maintaining zinc homeostasis in the pathogenic bacteria by binding to zinc and regulating the genes involved...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.03314

    authors: Kandari D,Gopalani M,Gupta M,Joshi H,Bhatnagar S,Bhatnagar R

    更新日期:2019-01-11 00:00:00

  • Explication of the Potential of 2-Hydroxy-4-Methoxybenzaldehyde in Hampering Uropathogenic Proteus mirabilis Crystalline Biofilm and Virulence.

    abstract::Proteus mirabilis is an important etiological agent of catheter-associated urinary tract infections (CAUTIs) owing to its efficient crystalline biofilm formation and virulence enzyme production. Hence, the present study explicated the antibiofilm and antivirulence efficacies of 2-hydroxy-4-methoxybenzaldehyde (HMB) ag...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02804

    authors: Durgadevi R,Abirami G,Alexpandi R,Nandhini K,Kumar P,Prakash S,Veera Ravi A

    更新日期:2019-12-10 00:00:00

  • Cholecystectomy Damages Aging-Associated Intestinal Microbiota Construction.

    abstract::The intestinal microbiome is essential in humans to maintain physiological balance and nutrition metabolism. Laparoscopic cholecystectomy due to gallstone disease and cholecystitis can cause intestinal microbial dysbiosis, and following bile acid metabolism dysfunction, positions the patient at high risk of colorectal...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01402

    authors: Wang W,Wang J,Li J,Yan P,Jin Y,Zhang R,Yue W,Guo Q,Geng J

    更新日期:2018-06-25 00:00:00

  • Bacterial Communities: Interactions to Scale.

    abstract::In the environment, bacteria live in complex multispecies communities. These communities span in scale from small, multicellular aggregates to billions or trillions of cells within the gastrointestinal tract of animals. The dynamics of bacterial communities are determined by pairwise interactions that occur between di...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.01234

    authors: Stubbendieck RM,Vargas-Bautista C,Straight PD

    更新日期:2016-08-08 00:00:00

  • Microbial diversity in a Venezuelan orthoquartzite cave is dominated by the Chloroflexi (Class Ktedonobacterales) and Thaumarchaeota Group I.1c.

    abstract::The majority of caves are formed within limestone rock and hence our understanding of cave microbiology comes from carbonate-buffered systems. In this paper, we describe the microbial diversity of Roraima Sur Cave (RSC), an orthoquartzite (SiO4) cave within Roraima Tepui, Venezuela. The cave contains a high level of m...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00615

    authors: Barton HA,Giarrizzo JG,Suarez P,Robertson CE,Broering MJ,Banks ED,Vaishampayan PA,Venkateswaran K

    更新日期:2014-11-26 00:00:00

  • Corrigendum: Integrating DNA Methylation and Gene Expression Data in the Development of the Soybean-Bradyrhizobium N2-Fixing Symbiosis.

    abstract::[This corrects the article on p. 518 in vol. 7, PMID: 27148207.]. ...

    journal_title:Frontiers in microbiology

    pub_type: 已发布勘误

    doi:10.3389/fmicb.2016.00952

    authors: Davis-Richardson AG,Russell JT,Dias R,McKinlay AJ,Canepa R,Fagen JR,Rusoff KT,Drew JC,Kolaczkowski B,Emerich DW,Triplett EW

    更新日期:2016-06-15 00:00:00

  • Silver As Antibacterial toward Listeria monocytogenes.

    abstract::Listeria monocytogenes is a serious foodborne pathogen that can contaminate food during processing and can grow during food shelf-life. New types of safe and effective food contact materials embedding antimicrobial agents, like silver, can play an important role in the food industry. The present work aimed at evaluati...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00307

    authors: Belluco S,Losasso C,Patuzzi I,Rigo L,Conficoni D,Gallocchio F,Cibin V,Catellani P,Segato S,Ricci A

    更新日期:2016-03-07 00:00:00

  • TaqMan qPCR for Quantification of Clonostachys rosea Used as a Biological Control Agent Against Fusarium graminearum.

    abstract::Clonostachys rosea is a biological control agent against Fusarium graminearum in small grain cereals and maize. Infections with F. graminearum do not only reduce the yield but, due to the production of mycotoxins, also affect the entire value chain of food and feed. In addition, production of other secondary metabolit...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01627

    authors: Gimeno A,Sohlberg E,Pakula T,Limnell J,Keller B,Laitila A,Vogelgsang S

    更新日期:2019-07-16 00:00:00

  • Arbuscular Mycorrhizal Fungi for the Biocontrol of Plant-Parasitic Nematodes: A Review of the Mechanisms Involved.

    abstract::Arbuscular mycorrhizal fungi (AMF) are obligate root symbionts that can protect their host plant against biotic stress factors such as plant-parasitic nematode (PPN) infection. PPN consist of a wide range of species with different life styles that can cause major damage in many important crops worldwide. Various mecha...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.01280

    authors: Schouteden N,De Waele D,Panis B,Vos CM

    更新日期:2015-11-17 00:00:00

  • Alpine Snow Algae Microbiome Diversity in the Coast Range of British Columbia.

    abstract::Snow algae blooms contain bacteria, fungi, and other microscopic organisms. We surveyed 55 alpine snow algae blooms, collecting a total of 68 samples, from 12 mountains in the Coast Range of British Columbia, Canada. We used microscopy and rDNA metabarcoding to document biodiversity and query species and taxonomic ass...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01721

    authors: Yakimovich KM,Engstrom CB,Quarmby LM

    更新日期:2020-07-28 00:00:00

  • Glass-forming property of hydroxyectoine is the cause of its superior function as a desiccation protectant.

    abstract::We were able to demonstrate that hydroxyectoine, in contrast to ectoine, is a good glass-forming compound. Fourier transform infrared and spin label electron spin resonance studies of dry ectoine and hydroxyectoine have shown that the superior glass-forming properties of hydroxyectoine result from stronger intermolecu...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00150

    authors: Tanne C,Golovina EA,Hoekstra FA,Meffert A,Galinski EA

    更新日期:2014-04-04 00:00:00

  • Changes in the Fungal Microbiome of Maize During Hermetic Storage in the United States and Kenya.

    abstract::Prior to harvest, maize kernels are invaded by a diverse population of fungal organisms that comprise the microbiome of the grain mass. Poor post-harvest practices and improper drying can lead to the growth of mycotoxigenic storage fungi and deterioration of grain quality. Hermetic storage bags are a low-cost technolo...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02336

    authors: Lane B,Sharma S,Niu C,Maina AW,Wagacha JM,Bluhm BH,Woloshuk CP

    更新日期:2018-10-02 00:00:00

  • Evidence of New Endemic Clusters of Human T-Cell Leukemia Virus (HTLV) Infection in Bahia, Brazil.

    abstract:Background:Salvador, Bahia (northeastern Brazil), has been identified as the epicenter of Human T-cell leukemia virus Human T-cell leukemia virus (HTLV) type 1 infection in the country. This study aims to estimate the rate of HTLV infection and the geographical distribution of this virus in this state. Methods:All HTL...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01002

    authors: Pereira FM,de Almeida MDCC,Santos FLN,Carreiro RP,Regis-Silva CG,Galvão-Castro B,Grassi MFR

    更新日期:2019-05-14 00:00:00

  • RskA Is a Dual Function Activator-Inhibitor That Controls SigK Activity Across Distinct Bacterial Genera.

    abstract::It has been previously shown that RskA, the anti-Sigma factor K of Mycobacterium tuberculosis, inhibits SigK and that mutations in RskA promote high expression of the SigK regulon. The latter observation led us to hypothesize that RskA mutations lead to loss of the anti-Sigma factor function. In this report, we used n...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.558166

    authors: Veyrier FJ,Nieves C,Lefrancois LH,Trigui H,Vincent AT,Behr MA

    更新日期:2020-09-09 00:00:00

  • Cell Wall Anchoring of the Campylobacter Antigens to Lactococcus lactis.

    abstract::Campylobacter jejuni is the most frequent cause of human food-borne gastroenteritis and chicken meat is the main source of infection. Recent studies showed that broiler chicken immunization against Campylobacter should be the most efficient way to lower the number of human infections by this pathogen. Induction of the...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00165

    authors: Kobierecka PA,Olech B,Książek M,Derlatka K,Adamska I,Majewski PM,Jagusztyn-Krynicka EK,Wyszyńska AK

    更新日期:2016-02-18 00:00:00

  • Metabolic Response of "Candidatus Accumulibacter Phosphatis" Clade II C to Changes in Influent P/C Ratio.

    abstract::The objective of this study was to investigate the ability of a culture highly enriched with the polyphosphate-accumulating organism, "Candidatus Accumulibacter phosphatis" clade IIC, to adjust their metabolism to different phosphate availabilities. For this purpose the biomass was cultivated in a sequencing batch rea...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.02121

    authors: Welles L,Abbas B,Sorokin DY,Lopez-Vazquez CM,Hooijmans CM,van Loosdrecht MC,Brdjanovic D

    更新日期:2017-01-05 00:00:00

  • Modeling Reveals the Role of Aging and Glucose Uptake Impairment in L1A1 Listeria monocytogenes Biofilm Life Cycle.

    abstract::Listeria monocytogenes is a food-borne pathogen that can persist in food processing plants by forming biofilms on abiotic surfaces. The benefits that bacteria can gain from living in a biofilm, i.e., protection from environmental factors and tolerance to biocides, have been linked to the biofilm structure. Different L...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02118

    authors: Balsa-Canto E,Vilas C,López-Núñez A,Mosquera-Fernández M,Briandet R,Cabo ML,Vázquez C

    更新日期:2017-11-01 00:00:00

  • QseC Mediates Osmotic Stress Resistance and Biofilm Formation in Haemophilus parasuis.

    abstract::Haemophilus parasuis is known as a commensal organism discovered in the upper respiratory tract of swine where the pathogenic bacteria survive in various adverse environmental stress. QseC, a histidine protein kinase of the two-component regulatory systems CheY/QseC, is involved in the environmental adaptation in bact...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00212

    authors: He L,Dai K,Wen X,Ding L,Cao S,Huang X,Wu R,Zhao Q,Huang Y,Yan Q,Ma X,Han X,Wen Y

    更新日期:2018-02-13 00:00:00

  • Oral Administration of Heat-Killed Mycobacterium manresensis Delays Progression toward Active Tuberculosis in C3HeB/FeJ Mice.

    abstract::Low-dose tolerance using heat-killed mycobacteria has been tested as a means of stopping progression toward active tuberculosis (TB) lesions in a human-like murine model using C3HeB/FeJ mice. In the present study, we studied the effect of different treatment schedules with heat-killed non-tuberculous-mycobacteria (NTM...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01482

    authors: Cardona P,Marzo-Escartín E,Tapia G,Díaz J,García V,Varela I,Vilaplana C,Cardona PJ

    更新日期:2016-01-05 00:00:00

  • Algae-based oral recombinant vaccines.

    abstract::Recombinant subunit vaccines are some of the safest and most effective vaccines available, but their high cost and the requirement of advanced medical infrastructure for administration make them impractical for many developing world diseases. Plant-based vaccines have shifted that paradigm by paving the way for recomb...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2014.00060

    authors: Specht EA,Mayfield SP

    更新日期:2014-02-17 00:00:00

  • The Glycerol Phosphatase Gpp2: A Link to Osmotic Stress, Sulfur Assimilation and Virulence in Cryptococcus neoformans.

    abstract::Cryptococcus neoformans is an opportunist fungal pathogen that causes meningoencephalitis in immunocompromised patients. During infection, this basidiomycete yeast has to adapt to several adverse conditions, especially nutrient availability. The interruption on various amino acid biosynthetic pathways and on amino aci...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02728

    authors: Martho KF,Brustolini OJB,Vasconcelos AT,Vallim MA,Pascon RC

    更新日期:2019-11-26 00:00:00