Early Trypanosoma cruzi Infection Triggers mTORC1-Mediated Respiration Increase and Mitochondrial Biogenesis in Human Primary Cardiomyocytes.

Abstract:

:Chagasic chronic cardiomyopathy is one of the most frequent and severe manifestations of Chagas disease, caused by the parasite Trypanosoma cruzi. The pathogenic and biochemical mechanisms responsible for cardiac lesions remain not completely understood, although it is clear that hypertrophy and subsequent heart dilatation is in part caused by the direct infection of cardiomyocytes. In this work, we evaluated the initial response of human cardiomyocytes to T. cruzi infection by transcriptomic profiling. Immediately after infection, cardiomyocytes dramatically change their gene expression patterns, up regulating most of the genes encoding for respiratory chain, oxidative phosphorylation and protein synthesis. We found that these changes correlate with an increase in basal and maximal respiration, as well as in spare respiratory capacity, which is accompanied by mitochondrial biogenesis pgc-1α independent. We also demonstrate that these changes are mediated by mTORC1 and reversed by rapamycin, resembling the molecular mechanisms described for the non-chagasic hypertrophic cardiomyopathy. The results of the present work identify that early during infection, the activation of mTORC1, mitochondrial biogenesis and improvement in oxidative phosphorylation are key biochemical changes that provide new insights into the host response to parasite infection and the pathogenesis of chronic chagasic cardiomyopathy. The finding that this phenotype can be reversed opens a new perspective in the treatment of Chagas disease, through the identification of host targets, and the use of combined parasite and host targeted therapies, in order to prevent chagasic cardiomyopathy.

journal_name

Front Microbiol

authors

Libisch MG,Faral-Tello P,Garg NJ,Radi R,Piacenza L,Robello C

doi

10.3389/fmicb.2018.01889

subject

Has Abstract

pub_date

2018-08-16 00:00:00

pages

1889

issn

1664-302X

journal_volume

9

pub_type

杂志文章
  • Differential Growth of Francisella tularensis, Which Alters Expression of Virulence Factors, Dominant Antigens, and Surface-Carbohydrate Synthases, Governs the Apparent Virulence of Ft SchuS4 to Immunized Animals.

    abstract::The gram-negative bacterium Francisella tularensis (Ft) is both a potential biological weapon and a naturally occurring microbe that survives in arthropods, fresh water amoeba, and mammals with distinct phenotypes in various environments. Previously, we used a number of measurements to characterize Ft grown in Brain-H...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01158

    authors: Holland KM,Rosa SJ,Kristjansdottir K,Wolfgeher D,Franz BJ,Zarrella TM,Kumar S,Sunagar R,Singh A,Bakshi CS,Namjoshi P,Barry EM,Sellati TJ,Kron SJ,Gosselin EJ,Reed DS,Hazlett KRO

    更新日期:2017-06-22 00:00:00

  • Diet-Induced Obesity Does Not Alter Tigecycline Treatment Efficacy in Murine Lyme Disease.

    abstract::Obese individuals more frequently suffer from infections, as a result of increased susceptibility to a number of bacterial pathogens. Furthermore, obesity can alter antibiotic treatment efficacy due to changes in drug pharmacokinetics which can result in under-dosing. However, studies on the treatment of bacterial inf...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00292

    authors: Pětrošová H,Eshghi A,Anjum Z,Zlotnikov N,Cameron CE,Moriarty TJ

    更新日期:2017-02-24 00:00:00

  • Characterization and Bio-Typing of Multidrug Resistance Plasmids From Uropathogenic Escherichia coli Isolated From Clinical Setting.

    abstract::Urinary tract infection is primarily caused by Escherichia coli. Multidrug resistance and their rapid dissemination in this pathogenic microbe complicate therapeutic strategies and threaten public health. Conjugation systems responsible for interbacterial transmission of antibiotic resistance are plasmid-encoded and c...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02913

    authors: Mukherjee SK,Mukherjee M

    更新日期:2019-12-18 00:00:00

  • Identity and mechanisms of alkane-oxidizing metalloenzymes from deep-sea hydrothermal vents.

    abstract::Six aerobic alkanotrophs (organism that can metabolize alkanes as their sole carbon source) isolated from deep-sea hydrothermal vents were characterized using the radical clock substrate norcarane to determine the metalloenzyme and reaction mechanism used to oxidize alkanes. The organisms studied were Alcanivorax sp. ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2013.00109

    authors: Bertrand EM,Keddis R,Groves JT,Vetriani C,Austin RN

    更新日期:2013-05-10 00:00:00

  • Swine-Derived Probiotic Lactobacillus plantarum Inhibits Growth and Adhesion of Enterotoxigenic Escherichia coli and Mediates Host Defense.

    abstract::Weaning stress renders piglets susceptible to pathogen infection, which leads to post-weaning diarrhea, a severe condition characterized by heavy diarrhea and mortality in piglets. Enterotoxigenic Escherichia coli (ETEC) is one of typical strains associated with post-weaning diarrhea. Thus, prevention and inhibition o...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01364

    authors: Wang J,Zeng Y,Wang S,Liu H,Zhang D,Zhang W,Wang Y,Ji H

    更新日期:2018-06-26 00:00:00

  • Microcalorimetric study of the growth of Streptococcus thermophilus in renneted milk.

    abstract::The growth of Streptococcus thermophilus ST12 (ST12) in liquid milk, reconstituted from low-heat skim milk powder reconstituted skim milk (RSM) and in RSM with rennet addition (r-RSM) at 40°C was monitored by microcalorimetry. It was shown that the growth rate of bacteria decreased in renneted samples in comparison wi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00079

    authors: Stulova I,Kabanova N,Kriščiunaite T,Adamberg K,Laht TM,Vilu R

    更新日期:2015-02-10 00:00:00

  • Differences in Ocular Complications Between Candida albicans and Non-albicans Candida Infection Analyzed by Epidemiology and a Mouse Ocular Candidiasis Model.

    abstract::Objectives:Candida species are a major cause of hospital infections, including ocular candidiasis, but few studies have examined the propensities of specific species to invade the eye or the unique immunological responses induced. This study examined the frequency and characteristics of species-specific Candida eye in...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02477

    authors: Abe M,Kinjo Y,Ueno K,Takatsuka S,Nakamura S,Ogura S,Kimura M,Araoka H,Sadamoto S,Shinozaki M,Shibuya K,Yoneyama A,Kaku M,Miyazaki Y

    更新日期:2018-10-17 00:00:00

  • Comparison of Bacterial and Fungal Composition and Their Chemical Interaction in Free Tropospheric Air and Snow Over an Entire Winter Season at Mount Sonnblick, Austria.

    abstract::We investigated the interactions of air and snow over one entire winter accumulation period as well as the importance of chemical markers in a pristine free-tropospheric environment to explain variation in a microbiological dataset. To overcome the limitations of short term bioaerosol sampling, we sampled the atmosphe...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00980

    authors: Els N,Greilinger M,Reisecker M,Tignat-Perrier R,Baumann-Stanzer K,Kasper-Giebl A,Sattler B,Larose C

    更新日期:2020-05-20 00:00:00

  • Preliminary Transcriptome Analysis of Mature Biofilm and Planktonic Cells of Salmonella Enteritidis Exposure to Acid Stress.

    abstract::Salmonella has emerged as a well-recognized food-borne pathogen, with many strains able to form biofilms and thus cause cross-contamination in food processing environments where acid-based disinfectants are widely encountered. In the present study, RNA sequencing was employed to establish complete transcriptome profil...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01861

    authors: Jia K,Wang G,Liang L,Wang M,Wang H,Xu X

    更新日期:2017-09-26 00:00:00

  • Increased Numbers of Culturable Inhibitory Bacterial Taxa May Mitigate the Effects of Batrachochytrium dendrobatidis in Australian Wet Tropics Frogs.

    abstract::Symbiotic bacterial communities resident on amphibian skin can benefit their hosts. For example, antibiotic production by community members can control the pathogen Batrachochytrium dendrobatidis (Bd) and it is possible for these community members to be used as probiotics to reduce infection levels. In the early 1990s...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01604

    authors: Bell SC,Garland S,Alford RA

    更新日期:2018-07-18 00:00:00

  • Draft Genome Analysis Offers Insights Into the Mechanism by Which Streptomyces chartreusis WZS021 Increases Drought Tolerance in Sugarcane.

    abstract::Drought directly affects sugarcane production. Plant growth-promoting bacteria have gained attention as growth promoters of plants under abiotic stresses. The present study focused on genome assessment of the plant-beneficial endophyte Streptomyces chartreusis WZS021 and its vital role in sugarcane plants under drough...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.03262

    authors: Wang Z,Solanki MK,Yu ZX,Yang LT,An QL,Dong DF,Li YR

    更新日期:2019-01-09 00:00:00

  • Modeling Reveals the Role of Aging and Glucose Uptake Impairment in L1A1 Listeria monocytogenes Biofilm Life Cycle.

    abstract::Listeria monocytogenes is a food-borne pathogen that can persist in food processing plants by forming biofilms on abiotic surfaces. The benefits that bacteria can gain from living in a biofilm, i.e., protection from environmental factors and tolerance to biocides, have been linked to the biofilm structure. Different L...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02118

    authors: Balsa-Canto E,Vilas C,López-Núñez A,Mosquera-Fernández M,Briandet R,Cabo ML,Vázquez C

    更新日期:2017-11-01 00:00:00

  • A Prospective Study on the Fermentation Landscape of Gaseous Substrates to Biorenewables Using Methanosarcina acetivorans Metabolic Model.

    abstract::The abundance of methane in shale gas and of other gases such as carbon monoxide, hydrogen, and carbon dioxide as chemical process byproducts has motivated the use of gas fermentation for bioproduction. Recent advances in metabolic engineering and synthetic biology allow for engineering of microbes metabolizing a vari...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01855

    authors: Nazem-Bokaee H,Maranas CD

    更新日期:2018-08-24 00:00:00

  • The Ubiquitin System and Kaposi's Sarcoma-Associated Herpesvirus.

    abstract::Ubiquitination is a post-translational modification in which one or more ubiquitin molecules are covalently linked to lysine residues of target proteins. The ubiquitin system plays a key role in the regulation of protein degradation, which contributes to cell signaling, vesicular trafficking, apoptosis, and immune reg...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00066

    authors: Ashizawa A,Higashi C,Masuda K,Ohga R,Taira T,Fujimuro M

    更新日期:2012-02-23 00:00:00

  • Degradation of Acephate and Its Intermediate Methamidophos: Mechanisms and Biochemical Pathways.

    abstract::Acephate is an organophosphate pesticide that has been widely used to control insect pests in agricultural fields for decades. However, its use has been partially restricted in many countries due to its toxic intermediate product methamidophos. Long term exposure to acephate and methamidophos in non-target organisms r...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2020.02045

    authors: Lin Z,Pang S,Zhang W,Mishra S,Bhatt P,Chen S

    更新日期:2020-08-18 00:00:00

  • Atypical Porcine Pestivirus as a Novel Type of Pestivirus in Pigs in China.

    abstract::Pestiviruses are highly variable RNA viruses. A growing number of novel pestiviruses has been discovered in domestic and wild species in the last two decades. Recently, a novel atypical porcine pestivirus (APPV) linked with the development of congenital tremor (CT) in neonatal pigs was described in Europe and the Amer...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00862

    authors: Yuan J,Han Z,Li J,Huang Y,Yang J,Ding H,Zhang J,Zhu M,Zhang Y,Liao J,Zhao M,Chen J

    更新日期:2017-05-11 00:00:00

  • Two Type VI Secretion Systems of Enterobacter cloacae Are Required for Bacterial Competition, Cell Adherence, and Intestinal Colonization.

    abstract::Enterobacter cloacae has emerged as an opportunistic pathogen in healthcare-associated infections. Analysis of the genomic sequences of several E. cloacae strains revealed the presence of genes that code for expression of at least one type VI secretion system (T6SS). Here, we report that E. cloacae strain ATCC 13047 c...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.560488

    authors: Soria-Bustos J,Ares MA,Gómez-Aldapa CA,González-Y-Merchand JA,Girón JA,De la Cruz MA

    更新日期:2020-09-24 00:00:00

  • Serotype Distribution and Antimicrobial Susceptibility of Streptococcus pneumoniae in Pre- and Post- PCV7/13 Eras, Taiwan, 2002-2018.

    abstract::In Taiwan, the 7-valent pneumococcal conjugate vaccine (PCV7) was introduced in 2006 and a PCV13 national childhood catchup program was implemented in 2013. To delineate the trend of serotype distribution and antimicrobial susceptibility following vaccination programs, we investigated a total of 1845 Streptococcus pne...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.557404

    authors: Wu CJ,Lai JF,Huang IW,Shiau YR,Wang HY,Lauderdale TL

    更新日期:2020-10-22 00:00:00

  • Biocatalytic desulfurization of thiophenic compounds and crude oil by newly isolated bacteria.

    abstract::Microorganisms possess enormous highly specific metabolic activities, which enable them to utilize and transform nearly every known chemical class present in crude oil. In this context, one of the most studied biocatalytic processes is the biodesulfurization (BDS) of thiophenic sulfur-containing compounds such as benz...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00112

    authors: Mohamed Mel-S,Al-Yacoub ZH,Vedakumar JV

    更新日期:2015-02-13 00:00:00

  • Enantioselective Utilization of D-Amino Acids by Deep-Sea Microorganisms.

    abstract::Microorganisms that utilize various D-amino acids (DAAs) were successfully isolated from deep-sea sediments. The isolates were phylogenetically assigned to Alphaproteobacteria, Gammmaproteobacteria, and Bacilli. Some of the isolates exhibited high enantioselective degradation activities to various DAAs. In particular,...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00511

    authors: Kubota T,Kobayashi T,Nunoura T,Maruyama F,Deguchi S

    更新日期:2016-04-19 00:00:00

  • Ecological Succession of Sulfur-Oxidizing Epsilon- and Gammaproteobacteria During Colonization of a Shallow-Water Gas Vent.

    abstract::In this study, we integrated geochemical measurements, microbial diversity surveys and physiological characterization of laboratory strains to investigate substrate-attached filamentous microbial biofilms at Tor Caldara, a shallow-water gas vent in the Tyrrhenian Sea. At this site, the venting gases are mainly compose...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02970

    authors: Patwardhan S,Foustoukos DI,Giovannelli D,Yücel M,Vetriani C

    更新日期:2018-12-06 00:00:00

  • Shedding Light on the Cell Biology of the Predatory Bacterium Bdellovibrio bacteriovorus.

    abstract::Bdellovibrio bacteriovorus is a predatory bacterium that feeds upon and proliferates inside other Gram-negative bacteria. Upon entry into the periplasmic space of the prey envelope, B. bacteriovorus initiates an exquisite developmental program in which it digests the host resources and grows as a filament, which event...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.03136

    authors: Laloux G

    更新日期:2020-01-21 00:00:00

  • Effects of Wilting and Lactobacillus plantarum Addition on the Fermentation Quality and Microbial Community of Moringa oleifera Leaf Silage.

    abstract::The objective of this study was to evaluate the effects of wilting and Lactobacillus plantarum (LP) addition on the silage fermentation quality and microbial community of Moringa oleifera Lam. leaf silage. Unwilted (direct-cut) or wilted M. oleifera leaves were prepared either with or without LP (1.0 × 106 cfu/g) foll...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01817

    authors: Wang Y,Wang C,Zhou W,Yang FY,Chen XY,Zhang Q

    更新日期:2018-08-06 00:00:00

  • The Impact of ackA, pta, and ackA-pta Mutations on Growth, Gene Expression and Protein Acetylation in Escherichia coli K-12.

    abstract::Acetate is a characteristic by-product of Escherichia coli K-12 growing in batch cultures with glucose, both under aerobic as well as anaerobic conditions. While the reason underlying aerobic acetate production is still under discussion, during anaerobic growth acetate production is important for ATP generation by sub...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00233

    authors: Schütze A,Benndorf D,Püttker S,Kohrs F,Bettenbrock K

    更新日期:2020-02-21 00:00:00

  • Antibacterial Potential of Jatropha curcas Synthesized Silver Nanoparticles against Food Borne Pathogens.

    abstract::The aqueous leaf extract of Jatropha curcas was used for the synthesis of silver nanoparticles (Jc-AgNps) which were further evaluated for its antibacterial potential against food borne pathogens. J. curcas leaf extract could synthesize stable silver nanoparticles (Zeta potential: -23.4 mV) with absorption band at 430...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01748

    authors: Chauhan N,Tyagi AK,Kumar P,Malik A

    更新日期:2016-11-08 00:00:00

  • SMRT Sequencing Revealed Mitogenome Characteristics and Mitogenome-Wide DNA Modification Pattern in Ophiocordyceps sinensis.

    abstract::Single molecule, real-time (SMRT) sequencing was used to characterize mitochondrial (mt) genome of Ophiocordyceps sinensis and to analyze the mt genome-wide pattern of epigenetic DNA modification. The complete mt genome of O. sinensis, with a size of 157,539 bp, is the fourth largest Ascomycota mt genome sequenced to ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01422

    authors: Kang X,Hu L,Shen P,Li R,Liu D

    更新日期:2017-07-27 00:00:00

  • Infectious Agents As Markers of Human Migration toward the Amazon Region of Brazil.

    abstract::Infectious agents are common companions of humans and since ancient times they follow human migration on their search for a better place to live. The study of paleomicrobiology was significantly improved in its accuracy of measurement with the constant development of better methods to detect and analyze nucleic acids....

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2017.01663

    authors: Ishak R,Machado LFA,Cayres-Vallinoto I,Guimarães Ishak MO,Vallinoto ACR

    更新日期:2017-08-31 00:00:00

  • Rapid in Vitro Quantification of S. aureus Biofilms on Vascular Graft Surfaces.

    abstract::Objectives: Increasing resistance of microorganisms and particularly tolerance of bacterial biofilms against antibiotics require the need for alternative antimicrobial substances. S. aureus is the most frequent pathogen causing vascular graft infections. In order to evaluate the antimicrobial efficacy, quantification ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02333

    authors: Herten M,Bisdas T,Knaack D,Becker K,Osada N,Torsello GB,Idelevich EA

    更新日期:2017-12-05 00:00:00

  • Characterization of Pseudomonas spp. and Associated Proteolytic Properties in Raw Milk Stored at Low Temperatures.

    abstract::Milk spoilage is caused by the presence of proteolytic enzymes produced by Pseudomonas spp. during storage at low temperatures. The aim of this study was to identify Pseudomonas spp. in raw milk and investigate their associated proteolytic properties at low temperatures. Raw milk samples (n = 87) were collected from 8...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02158

    authors: Meng L,Zhang Y,Liu H,Zhao S,Wang J,Zheng N

    更新日期:2017-11-08 00:00:00

  • Diversity and Expression of Bacterial Metacaspases in an Aquatic Ecosystem.

    abstract::Metacaspases are distant homologs of metazoan caspase proteases, implicated in stress response, and programmed cell death (PCD) in bacteria and phytoplankton. While the few previous studies on metacaspases have relied on cultured organisms and sequenced genomes, no studies have focused on metacaspases in a natural set...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01043

    authors: Asplund-Samuelsson J,Sundh J,Dupont CL,Allen AE,McCrow JP,Celepli NA,Bergman B,Ininbergs K,Ekman M

    更新日期:2016-07-06 00:00:00