Two Type VI Secretion Systems of Enterobacter cloacae Are Required for Bacterial Competition, Cell Adherence, and Intestinal Colonization.

Abstract:

:Enterobacter cloacae has emerged as an opportunistic pathogen in healthcare-associated infections. Analysis of the genomic sequences of several E. cloacae strains revealed the presence of genes that code for expression of at least one type VI secretion system (T6SS). Here, we report that E. cloacae strain ATCC 13047 codes for two functional T6SS named T6SS-1 and T6SS-2. T6SS-1 and T6SS-2 were preferentially expressed in tryptic soy broth and tissue culture medium (DMEM), respectively. Mutants in T6SS-1-associated genes clpV1 and hcp1 significantly affected their ability of inter- and intra-bacterial killing indicating that T6SS-1 is required for bacterial competition. In addition, the Hcp effector protein was detected in supernatants of E. cloacae cultures and a functional T6SS-1 was required for the secretion of this protein. A clpV2 mutant was impaired in both biofilm formation and adherence to epithelial cells, supporting the notion that these phenotypes are T6SS-2 dependent. In vivo data strongly suggest that both T6SSs are required for intestinal colonization because single and double mutants in clpV1 and clpV2 genes were defective in gut colonization in mice. We conclude that the two T6SSs are involved in the pathogenesis scheme of E. cloacae with specialized functions in the interaction with other bacteria and with host cells.

journal_name

Front Microbiol

authors

Soria-Bustos J,Ares MA,Gómez-Aldapa CA,González-Y-Merchand JA,Girón JA,De la Cruz MA

doi

10.3389/fmicb.2020.560488

subject

Has Abstract

pub_date

2020-09-24 00:00:00

pages

560488

issn

1664-302X

journal_volume

11

pub_type

杂志文章
  • Changing Perspectives on the Role of DnaA-ATP in Orisome Function and Timing Regulation.

    abstract::Bacteria, like all cells, must precisely duplicate their genomes before they divide. Regulation of this critical process focuses on forming a pre-replicative nucleoprotein complex, termed the orisome. Orisomes perform two essential mechanical tasks that configure the unique chromosomal replication origin, oriC to star...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2019.02009

    authors: Leonard AC,Rao P,Kadam RP,Grimwade JE

    更新日期:2019-08-29 00:00:00

  • Morphologically Different Pectobacterium brasiliense Bacteriophages PP99 and PP101: Deacetylation of O-Polysaccharide by the Tail Spike Protein of Phage PP99 Accompanies the Infection.

    abstract::Soft rot caused by numerous species of Pectobacterium and Dickeya is a serious threat to the world production of potatoes. The application of bacteriophages to combat bacterial infections in medicine, agriculture, and the food industry requires the selection of comprehensively studied lytic phages and the knowledge of...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.03147

    authors: Lukianova AA,Shneider MM,Evseev PV,Shpirt AM,Bugaeva EN,Kabanova AP,Obraztsova EA,Miroshnikov KK,Senchenkova SN,Shashkov AS,Toschakov SV,Knirel YA,Ignatov AN,Miroshnikov KA

    更新日期:2020-01-23 00:00:00

  • Phylogeography and Ecological Niche Shape the Cichlid Fish Gut Microbiota in Central American and African Lakes.

    abstract::Cichlid fishes, with their repeated colonization of lakes and subsequent radiations at different scales of phylogenetic and ecological diversification, offer an excellent model system to understand the factors shaping the host-gut microbiota association in nature. Here, we characterized the gut microbiota of the Amphi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02372

    authors: Baldo L,Riera JL,Salzburger W,Barluenga M

    更新日期:2019-10-15 00:00:00

  • Genomic Characterization and Probiotic Potency of Bacillus sp. DU-106, a Highly Effective Producer of L-Lactic Acid Isolated From Fermented Yogurt.

    abstract::Bacillus sp. DU-106, a newly isolated member of Bacillus cereus group, exhibits the predominant ability to produce L-lactic acid. The probiotic potency of test strain revealed its survivability at acidic pH, bile salts and viability in simulated gastric juice in vitro. The acute oral toxicity test indicated its no tox...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02216

    authors: Li P,Tian W,Jiang Z,Liang Z,Wu X,Du B

    更新日期:2018-09-20 00:00:00

  • Semi-Quantification of Total Campylobacter and Salmonella During Egg Incubations Using a Combination of 16S rDNA and Specific Pathogen Primers for qPCR.

    abstract::Rapid molecular techniques that evaluate eggs for the presence of foodborne pathogens is an essential component to poultry food safety monitoring. Interestingly, it is not just table eggs that contribute to outbreaks of foodborne disease. Broiler layer production actively contributes to sustaining of foodborne pathoge...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02454

    authors: Rothrock MJ Jr,Feye KM,Kim SA,Park SH,Locatelli A,Hiett KL,Gamble J,Sellers H,Ricke SC

    更新日期:2018-11-02 00:00:00

  • Reduced vancomycin susceptibility in porcine ST9 MRSA isolates.

    abstract::Porcine strains of livestock-associated methicillin resistant Staphylococcus aureus (LA-MRSA) have been recognized in many countries and have been shown to be able to cause human infection. Resistance to non-beta lactam antibiotics has been reported but non-susceptibility to vancomycin, which is known to occur in huma...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2013.00316

    authors: Kwok GM,O'Donoghue MM,Doddangoudar VC,Ho J,Boost MV

    更新日期:2013-10-25 00:00:00

  • Contributions of SpoT Hydrolase, SpoT Synthetase, and RelA Synthetase to Carbon Source Diauxic Growth Transitions in Escherichia coli.

    abstract::During the diauxic shift, Escherichia coli exhausts glucose and adjusts its expression pattern to grow on a secondary carbon source. Transcriptional profiling studies of glucose-lactose diauxic transitions reveal a key role for ppGpp. The amount of ppGpp depends on RelA synthetase and the balance between a strong SpoT...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01802

    authors: Fernández-Coll L,Cashel M

    更新日期:2018-08-03 00:00:00

  • Evidence That Microorganisms at the Animal-Water Interface Drive Sea Star Wasting Disease.

    abstract::Sea star wasting (SSW) disease describes a condition affecting asteroids that resulted in significant Northeastern Pacific population decline following a mass mortality event in 2013. The etiology of SSW is unresolved. We hypothesized that SSW is a sequela of microbial organic matter remineralization near respiratory ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.610009

    authors: Aquino CA,Besemer RM,DeRito CM,Kocian J,Porter IR,Raimondi PT,Rede JE,Schiebelhut LM,Sparks JP,Wares JP,Hewson I

    更新日期:2021-01-06 00:00:00

  • Viruses and miRNAs: More Friends than Foes.

    abstract::There is evidence that eukaryotic miRNAs (hereafter called host miRNAs) play a role in the replication and propagation of viruses. Expression or targeting of host miRNAs can be involved in cellular antiviral responses. Most times host miRNAs play a role in viral life-cycles and promote infection through complex regula...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2017.00824

    authors: Bruscella P,Bottini S,Baudesson C,Pawlotsky JM,Feray C,Trabucchi M

    更新日期:2017-05-15 00:00:00

  • GlnR Dominates Rifamycin Biosynthesis by Activating the rif Cluster Genes Transcription Both Directly and Indirectly in Amycolatopsis mediterranei.

    abstract::Because of the remarkable efficacy in treating mycobacterial infections, rifamycin and its derivatives are still first-line antimycobacterial drugs. It has been intensely studied to increase rifamycin yield from Amycolatopsis mediterranei, and nitrate is found to provide a stable and remarkable stimulating effect on t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00319

    authors: Liu X,Liu Y,Lei C,Zhao G,Wang J

    更新日期:2020-03-03 00:00:00

  • Regulation of mRNA Stability During Bacterial Stress Responses.

    abstract::Bacteria have a remarkable ability to sense environmental changes, swiftly regulating their transcriptional and posttranscriptional machinery as a response. Under conditions that cause growth to slow or stop, bacteria typically stabilize their transcriptomes in what has been shown to be a conserved stress response. In...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2020.02111

    authors: Vargas-Blanco DA,Shell SS

    更新日期:2020-09-09 00:00:00

  • Diversity and Expression of Bacterial Metacaspases in an Aquatic Ecosystem.

    abstract::Metacaspases are distant homologs of metazoan caspase proteases, implicated in stress response, and programmed cell death (PCD) in bacteria and phytoplankton. While the few previous studies on metacaspases have relied on cultured organisms and sequenced genomes, no studies have focused on metacaspases in a natural set...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01043

    authors: Asplund-Samuelsson J,Sundh J,Dupont CL,Allen AE,McCrow JP,Celepli NA,Bergman B,Ininbergs K,Ekman M

    更新日期:2016-07-06 00:00:00

  • A Recombinant Subviral Particle-Based Vaccine Protects Magpie (Pica pica) Against West Nile Virus Infection.

    abstract::The mosquito-borne West Nile virus (WNV) is a highly neurovirulent Flavivirus currently representing an emergent zoonotic concern. WNV cycles in nature between mosquito vectors and birds that act as amplifier hosts and play an essential role in virus ecology, being, thus, WNV a threat to many species. Availability of ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01133

    authors: Jiménez de Oya N,Escribano-Romero E,Camacho MC,Blazquez AB,Martín-Acebes MA,Höfle U,Saiz JC

    更新日期:2019-06-05 00:00:00

  • Glycan cross-feeding activities between bifidobacteria under in vitro conditions.

    abstract::Bifidobacteria colonize the gut of various mammals, including humans, where they may metabolize complex, diet-, and host-derived carbohydrates. The glycan-associated metabolic features encoded by bifidobacteria are believed to be strongly influenced by cross-feeding activities due to the co-existence of strains with d...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01030

    authors: Turroni F,Özcan E,Milani C,Mancabelli L,Viappiani A,van Sinderen D,Sela DA,Ventura M

    更新日期:2015-09-24 00:00:00

  • Seasonal dynamics of bacterial and archaeal methanogenic communities in flooded rice fields and effect of drainage.

    abstract::We studied the resident (16S rDNA) and the active (16S rRNA) members of soil archaeal and bacterial communities during rice plant development by sampling three growth stages (vegetative, reproductive and maturity) under field conditions. Additionally, the microbial community was investigated in two non-flooded fields ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00752

    authors: Breidenbach B,Conrad R

    更新日期:2015-01-08 00:00:00

  • Resistance and Resilience of Sulfidogenic Communities in the Face of the Specific Inhibitor Perchlorate.

    abstract::Hydrogen sulfide is a toxic and corrosive gas, produced by the activity of sulfate-reducing microorganisms (SRM). Owing to the environmental, economic and human-health consequences of sulfide, there is interest in developing specific inhibitors of SRM. Recent studies have identified perchlorate as a promising emerging...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00654

    authors: Stoeva MK,Nalula G,Garcia N,Cheng Y,Engelbrektson AL,Carlson HK,Coates JD

    更新日期:2019-04-02 00:00:00

  • Mucosal Prevalence and Interactions with the Epithelium Indicate Commensalism of Sutterella spp.

    abstract::Sutterella species have been frequently associated with human diseases, such as autism, Down syndrome, and inflammatory bowel disease (IBD), but the impact of these bacteria on health still remains unclear. Especially the interactions of Sutterella spp. with the host are largely unknown, despite of the species being h...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01706

    authors: Hiippala K,Kainulainen V,Kalliomäki M,Arkkila P,Satokari R

    更新日期:2016-10-26 00:00:00

  • Characterization of saltern based Streptomyces sp. and statistical media optimization for its improved antibacterial activity.

    abstract::A moderately halotolerant Streptomyces strain, designated JAJ13 was characterized and a culture medium was statistically optimized to improve its antibacterial activity. Based on the phenotypic and molecular characteristics, strain JAJ13 was identified as a moderately halotolerant Streptomyces sp. JAJ13. Novelty of th...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00753

    authors: Rajeswari P,Jose PA,Amiya R,Jebakumar SR

    更新日期:2015-01-21 00:00:00

  • Genetic Dissection of the Regulatory Network Associated with High c-di-GMP Levels in Pseudomonas putida KT2440.

    abstract::Most bacteria grow in nature forming multicellular structures named biofilms. The bacterial second messenger cyclic diguanosine monophosphate (c-di-GMP) is a key player in the regulation of the transition from planktonic to sessile lifestyles and this regulation is crucial in the development of biofilms. In Pseudomona...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01093

    authors: Ramos-González MI,Travieso ML,Soriano MI,Matilla MA,Huertas-Rosales Ó,Barrientos-Moreno L,Tagua VG,Espinosa-Urgel M

    更新日期:2016-07-20 00:00:00

  • The Influence of Viable Cells and Cell-Free Extracts of Lactobacillus casei on Volatile Compounds and Polyphenolic Profile of Elderberry Juice.

    abstract::In this study, four strains of Lactobacillus casei, as viable cells or cell-free extracts (CFE), were added to elderberry juice in order to evaluate their effect on phenolic and aromatic profile. Two of them were able to grow in juice while the others showed zero-growth. The same strains were lysed and added as extrac...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02784

    authors: Ricci A,Levante A,Cirlini M,Calani L,Bernini V,Del Rio D,Galaverna G,Neviani E,Lazzi C

    更新日期:2018-11-20 00:00:00

  • Diversity of Mobile Genetic Elements in the Mitogenomes of Closely Related Fusarium culmorum and F. graminearum sensu stricto Strains and Its Implication for Diagnostic Purposes.

    abstract::Much of the mitogenome variation observed in fungal lineages seems driven by mobile genetic elements (MGEs), which have invaded their genomes throughout evolution. The variation in the distribution and nucleotide diversity of these elements appears to be the main distinction between different fungal taxa, making them ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01002

    authors: Kulik T,Brankovics B,van Diepeningen AD,Bilska K,Żelechowski M,Myszczyński K,Molcan T,Stakheev A,Stenglein S,Beyer M,Pasquali M,Sawicki J,Wyrȩbek J,Baturo-Cieśniewska A

    更新日期:2020-05-25 00:00:00

  • Description and Comparative Genomics of Macrococcus caseolyticus subsp. hominis subsp. nov., Macrococcus goetzii sp. nov., Macrococcus epidermidis sp. nov., and Macrococcus bohemicus sp. nov., Novel Macrococci From Human Clinical Material With Virulence P

    abstract::The genus Macrococcus is a close relative of the genus Staphylococcus. Whilst staphylococci are widespread as human pathogens, macrococci have not yet been reported from human clinical specimens. Here we investigated Gram-positive and catalase-positive cocci recovered from human clinical material and identified as Mac...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01178

    authors: Mašlaňová I,Wertheimer Z,Sedláček I,Švec P,Indráková A,Kovařovic V,Schumann P,Spröer C,Králová S,Šedo O,Krištofová L,Vrbovská V,Füzik T,Petráš P,Zdráhal Z,Ružičková V,Doškař J,Pantuček R

    更新日期:2018-06-13 00:00:00

  • Lactic Acid Treatment of Cereals and Dietary Phytase Modified Fecal Microbiome Composition Without Affecting Expression of Virulence Factor Genes in Growing Pigs.

    abstract::Besides the major nutrients, phosphorus (P) is an essential mineral for both the host animal and the porcine gut microbiota. Different strategies including phytase supplementation and more recently lactic acid (LA) are used to enhance the P availability from cereals in pig diets; however, their impact on the gut micro...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02345

    authors: Klinsoda J,Vötterl J,Zebeli Q,Metzler-Zebeli BU

    更新日期:2019-10-15 00:00:00

  • Pseudomonas orientalis F9: A Potent Antagonist against Phytopathogens with Phytotoxic Effect in the Apple Flower.

    abstract::In light of public concerns over the use of pesticides and antibiotics in plant protection and the subsequent selection for spread of resistant bacteria in the environment, it is inevitable to broaden our knowledge about viable alternatives, such as natural antagonists and their mode of action. The genus Pseudomonas i...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00145

    authors: Zengerer V,Schmid M,Bieri M,Müller DC,Remus-Emsermann MNP,Ahrens CH,Pelludat C

    更新日期:2018-02-09 00:00:00

  • Microbiota Succession and Chemical Composition Involved in the Radish Fermentation Process in Different Containers.

    abstract::Traditional Chinese fermented vegetables are a type of brine-salted fermented vegetable product. During the spontaneous fermentation, various compounds are produced, degraded, and converted, influencing the quality of the fermented pickle. To ascertain the effect of different containers on the fermentation process of ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00445

    authors: Liu L,She X,Chen X,Qian Y,Tao Y,Li Y,Guo S,Xiang W,Liu G,Rao Y

    更新日期:2020-04-03 00:00:00

  • Expanding the Current Knowledge About the Role of Interleukin-10 to Major Concerning Bacteria.

    abstract::Interleukin-10 (IL-10) is one of the most important anti-inflammatory cytokine produced during bacterial infection. Two related phenomena explain the importance of IL-10 production in this context: first, the wide range of cells able to produce this cytokine and second, the wide effects that it causes on target cells....

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2018.02047

    authors: Peñaloza HF,Noguera LP,Riedel CA,Bueno SM

    更新日期:2018-09-18 00:00:00

  • The Regulator PltZ Regulates a Putative ABC Transporter System PltIJKNOP of Pseudomonas aeruginosa ATCC 27853 in Response to the Antimicrobial 2,4-Diacetylphloroglucinol.

    abstract::Pseudomonas aeruginosa is an opportunistic pathogen commonly infecting immunocompromised patients with diseases like cystic fibrosis (CF) and cancers and has high rates of recurrence and mortality. The treatment efficacy can be significantly worsened by the multidrug resistance (MDR) of P. aeruginosa, and there is inc...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01423

    authors: Guo DD,Luo LM,Ma HL,Zhang SP,Xu H,Zhang H,Wang Y,Yuan Y,Wang Z,He YX

    更新日期:2020-07-08 00:00:00

  • Diversity, Abundance, and Niche Differentiation of Ammonia-Oxidizing Prokaryotes in Mud Deposits of the Eastern China Marginal Seas.

    abstract::The eastern China marginal seas (ECMS) are prominent examples of river-dominated ocean margins, whose most characteristic feature is the existence of isolated mud patches on sandy sediments. Ammonia-oxidizing prokaryotes play a crucial role in the nitrogen cycles of many marine environments, including marginal seas. H...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00137

    authors: Yu S,Yao P,Liu J,Zhao B,Zhang G,Zhao M,Yu Z,Zhang XH

    更新日期:2016-02-12 00:00:00

  • Weak Organic Acids Decrease Borrelia burgdorferi Cytoplasmic pH, Eliciting an Acid Stress Response and Impacting RpoN- and RpoS-Dependent Gene Expression.

    abstract::The spirochete Borrelia burgdorferi survives in its tick vector, Ixodes scapularis, or within various hosts. To transition between and survive in these distinct niches, B. burgdorferi changes its gene expression in response to environmental cues, both biochemical and physiological. Exposure of B. burgdorferi to weak m...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01734

    authors: Dulebohn DP,Richards CL,Su H,Lawrence KA,Gherardini FC

    更新日期:2017-09-29 00:00:00

  • May the Phage be With You? Prophage-Like Elements in the Genomes of Soft Rot Pectobacteriaceae: Pectobacterium spp. and Dickeya spp.

    abstract::Soft Rot Pectobacteriaceae (SRP; Pectobacterium spp. and Dickeya spp., formerly known as pectinolytic Erwinia spp.) are necrotrophic bacterial pathogens infecting a large number of plant species worldwide, including agriculturally-important crops. Despite the SRP importance in agriculture, little is known about the ba...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00138

    authors: Czajkowski R

    更新日期:2019-02-14 00:00:00