Abstract：
BACKGROUND:Photosensitizers in nanocarriers have been investigated for antimicrobial Photodynamic Therapy (aPDT). However, most studies are focused against microorganisms in planktonic or monospecies biofilm. Thus, this in vitro study evaluated the effect of aPDT using Chloroaluminium phthalocyanine (ClAlPc) in cationic nanoemulsion (NE) against Candida albicans, Candida glabrata and Streptococcus mutans grown as multispecies biofilm. METHODS:Standard suspensions of each microorganism were added into wells of a microtiter plate for biofilm growth for 48 h in a candle jar. The biofilms were incubated with ClAlPc in cationic NE at 31.8 μM for 30 min and illuminated with red light fluence of 39.3 J/cm2 (P+L+ group). Additional samples were treated only with photosensitizer (P+L-) or red light (P-L+) or neither (P-L-, control group). aPDT efficacy was assessed by colony quantification, biofilm's metabolic activity, total biomass, and confocal microscopy. Data were analyzed by ANOVA/Welch and post-hoc Tukey/Games-Howell tests (α = 0.05). RESULTS:aPDT (P+L+) reduced the colony count in 1.30 to 2.24 lg10 and the metabolic activity in 53.7% compared with the control group (P-L-). The total biomass showed no statistical difference among the groups. The confocal microscopy analyzes showed uptake of the PS in the biofilm, and dead cells were observed in the biofilm treated with aPDT. CONCLUSION:aPDT mediated by ClAlPc in cationic NE promoted photoinactivation of the multispecies biofilm, which was confirmed by colony quantification, metabolic activity, and confocal microscopy. However, the total biomass of the biofilm was not affected by the treatment.
journal_name
Photodiagnosis Photodyn Therjournal_title
Photodiagnosis and photodynamic therapyauthors
Trigo-Gutierrez JK,Sanitá PV,Tedesco AC,Pavarina AC,Mima EGOdoi
10.1016/j.pdpdt.2018.10.005subject
Has Abstractpub_date
2018-12-01 00:00:00pages
212-219eissn
1572-1000issn
1873-1597pii
S1572-1000(18)30097-8journal_volume
24pub_type
杂志文章abstract：:Metal-semiconductor core-shell type Pd@SiO2 nanoparticles (NPs) were successfully synthesized by Stober's method and the product was characterized by UV-vis, XRD, FT-IR, SEM, HR-TEM and EDX techniques. In vitro Photodynamic activity and DNA binding studies of Pd@SiO2 core shell nanoparticles were studied. Cell viabili...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2019.03.008
更新日期：2019-06-01 00:00:00
abstract：:Lichen sclerosus et atrophicus (LSA) is disease of skin and mucosa, its pathogenesis remains unknown. Itching, pain and burning sensations and atrophy of vulva impair quality of life. Treatment is symptomatic. We report case of 30-year old woman with lesions in vulva in which series of topical PDT were carried out. We...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2012.02.002
更新日期：2012-06-01 00:00:00
abstract：:In this review article we described the applications of various nanoparticles that can be used for photodynamic therapy (PDT), such as: plasmonic nanoparticles, quantum dots and upconversion nanoparticles. In comparison with typical organic photosensitizers such as hematoporphyrins, they exhibit higher photostability ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章,评审
doi：10.1016/j.pdpdt.2019.03.016
更新日期：2019-06-01 00:00:00
abstract：AIM:This study aimed to compare the antibacterial effect of antimicrobial photodynamic therapy (aPDT) by use of light emitting diode (LED) and diode laser light sources with toluidine blue (TBO) photosensitizer on Enterococcus faecalis (E. faecalis) biofilm in root canals of extracted single-canal human teeth. METHODS...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.102036
更新日期：2020-12-01 00:00:00
abstract：:Vibrio parahaemolyticus (V. parahaemolyticus) is currently a major cause of bacterial diarrhoea associated with seafood consumption. The objective of this study was to determine the inactivation effect of curcumin-mediated photodynamic action on V. parahaemolyticus. First of all, V. parahaemolyticus suspended in PBS b...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2016.05.004
更新日期：2016-09-01 00:00:00
abstract：AIM:To investigate the effects of ALA-PDT on biofilm structure, virulence factor secretion, and quorum sensing (QS) in Pseudomonas aeruginosa. MATERIALS AND METHODS:We used confocal laser scanning microscopy (CLSM), an XTT assay, scanning electron microscopy (SEM), a virulence factor assay and qRT-PCR in this study. ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2018.07.005
更新日期：2018-12-01 00:00:00
abstract：BACKGROUND:Previous in vivo studies on photodynamic therapy (PDT)-treated, high cellular density tumors showed evidences of a bystander effect accompanying the therapy, cellular death continuing beyond the limits of the photochemical reactions in time and space. This process is generated by the initially damaged cells ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2012.03.001
更新日期：2012-12-01 00:00:00
abstract：BACKGROUND:Multidrug resistance (MDR) of breast cancer is a major obstacle in chemotherapy of cancer treatments. Recently the anti-tumor effects of Chlorin e6 (Ce6) mediated photodynamic therapy (Ce6-PDT) were reported in skin cancer and hepatoma in vitro. However, its therapeutic potential in killing human breast canc...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2016.07.004
更新日期：2016-12-01 00:00:00
abstract：:Photodynamic therapy (PDT) is a clinically approved therapeutic modality used for the management of several types of tumors as well as non-malignant diseases. Most of the effects of this treatment regimen result from direct action of singlet oxygen and reactive oxygen species. However, accumulating evidence indicates ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/S1572-1000(05)00098-0
更新日期：2005-12-01 00:00:00
abstract：BACKGROUND:an accurate measurement of anterior chamber depth (ACD) both in eyes that underwent corneal refractive surgery (CRS) and in eyes that did not, can be crucial, as in case of intraocular lens (IOL) calculation in eyes that need to undergo cataract extraction, or during the evaluation of glaucomatous patients. ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.101976
更新日期：2020-12-01 00:00:00
abstract：OBJECTIVE:To analyze the clinical efficacy of hematoporphyrin monomethyl ether photodynamic therapy (HMME-PDT) in treating port-wine stains (PWS), and its clinical operation skills and operation essentials. METHOD:A total of 16 cases of PWS with negative HMME skin test results were included in this study. Their treate...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章,评审
doi：10.1016/j.pdpdt.2017.09.013
更新日期：2017-12-01 00:00:00
abstract：PURPOSE:To investigate the autofluorescence (AF) characteristics of punctate inner choroidopathy (PIC) and to evaluate the progression rate of retinal pigment epithelium (RPE) atrophy lesions in PIC using confocal scanning laser ophthalmoscopy. METHODS:Twenty-two eyes of 14 PIC cases and 21 eyes of 21 non-proliferativ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2014.07.002
更新日期：2014-12-01 00:00:00
abstract：SIGNIFICANCE:The estimation of tissue oxygenation is vital in the diagnosis and therapeutic evaluation of a huge assortment of diseases. However, the hyperspectral (HS) imaging system is a rising innovation that can be utilized to build a highly sensitive, non-invasive, and tissue hemoglobin immersion map. OBJECTIVE:A...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2021.102190
更新日期：2021-01-25 00:00:00
abstract：BACKGROUND:To evaluate the effect of the first acute anterior uveitis (AAU) attack on the choroid by using an image binarization tool on images from enhanced depth imaging (EDI)-optical coherence tomography (OCT) scans in patients with ankylosing spondylitis (AS). METHODS:In this study, three groups were formed: uveit...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.101778
更新日期：2020-09-01 00:00:00
abstract：:Perifolliculitis capitis abscedens et suffodiens (PCAS), also known as dissecting cellulitis of the scalp (DCS), is a rare, chronic, suppurative inflammatory disease of the scalp which may evolve into cicatricial alopecia. Its refractory nature has a detrimental impact on patients' quality of life. Here, we report fiv...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.101660
更新日期：2020-06-01 00:00:00
abstract：BACKGROUND:Bacterial resistance is a problem during the process of healing of infected wounds. As a therapy, photodynamic therapy (PDT) has broad-spectral antibacterial activity and non-selective action, which makes it possible to deal with antibiotic resistance.. Methylene blue is a commonly used medicine, but it is r...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.102051
更新日期：2020-12-01 00:00:00
abstract：BACKGROUND:Microorganisms treated by photo-activated disinfection (PAD) in combined periodontal-endodontic (perio-endo) lesions would be exposed to sub-lethal doses of PAD (sPAD). This study evaluated the effect of sPAD using toluidine blue O (TBO) in combination with diode laser irradiation on the growth and biofilm-f...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2016.08.013
更新日期：2016-12-01 00:00:00
abstract：:Today, the widespread use of laser instruments in various fields has resulted in many accidental retinal injuries. Here we describe a rare clinical case of full-thickness macular hole (MH) and focal choroidal excavation (FCE) caused by a 1064-nm Q-switched Nd:YAG laser. After pars plana vitrectomy and the following 14...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2017.07.001
更新日期：2017-09-01 00:00:00
abstract：BACKGROUND:Photodynamic therapy has apart from a direct cytotoxic effect also immunomodulatory properties. The aim of our study was to investigate how photodynamic therapy with 5-aminolevulinic acid (ALA-PDT) in sublethal doses influences the secretion of interleukins 6, 8 and 10 from colon cancer cells in vitro. METH...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2018.02.021
更新日期：2018-06-01 00:00:00
abstract：BACKGROUND:One of the most frequent type of malignant skin lesion (almost 95 percent of all skin tumours) is basal cell carcinoma (BCC). It is often treated by radiotherapy using ionizing radiation as well by photodynamic therapy (PDT) which is a selective method directed only on cancer cells and well tolerated by pati...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.101845
更新日期：2020-09-01 00:00:00
abstract：BACKGROUND/AIM:Laser-based photodynamic endoscopic diagnosis (LPDED) is a type of endoscopic diagnosis that uses the fluorescence caused by the photochemical reaction that occurs when a fluorescent substance is irradiated by a light of a specific wavelength. Although 5-aminolevulinic acid (5-ALA) can detect early gastr...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2018.02.005
更新日期：2018-06-01 00:00:00
abstract：:We have more than 10 years experience in the Photobiology Unit, in the use of topical photodynamic therapy (PDT) for non-melanoma skin cancer and other skin diseases. During this time we have undertaken approximately 5000 treatments and this article details the practical aspects of how we treat Bowen's disease with to...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2009.04.001
更新日期：2009-03-01 00:00:00
abstract：:Hidradenitis suppurativa (HS) is a chronic, inflammatory, and recurrent skin disease. Surgical excision of wounds appears to be the only curative treatment for the prevention of recurrence of moderate to severe stages. Magnetic resonance imaging (MRI) is a standard reference examination for the detection of HS peri-an...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2018.05.007
更新日期：2018-09-01 00:00:00
abstract：BACKGROUND:The treatment of periodontal disease focuses on eradication or suppression of the pathogenic microbiota within the periodontal pocket. There are some mechanical and chemical ways, and recently antimicrobial photodynamic therapy to eliminate the bacteria. The aim of this in vitro study was to compare the effe...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2016.04.003
更新日期：2016-09-01 00:00:00
abstract：BACKGROUND:Increasing resistance of oral pathogens to conventional antibacterial agents has resulted to find alternative therapies to overcome resistance development problems; hence this in vitro study was carried out to investigate the efficacy of photoelimination of Streptococcus mutans with two methods of photodynam...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2013.07.002
更新日期：2013-12-01 00:00:00
abstract：BACKGROUND:Photodynamic therapy (PDT) is a highly efficient approach for tumour therapy, though it also has its drawbacks, too. There are multiple mechanisms involved in cell death regulation that can be successfully targeted for improvement of PDT in particular cases. We assumed, however, that the potential to manage ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2013.04.003
更新日期：2013-12-01 00:00:00
abstract：OBJECTIVE:The aim of the present 6-month follow-up study was to assess the effect of mechanical debridement (MD) with adjunct antimicrobial photodynamic therapy (aPDT) in the treatment of peri-implant diseases in type-2 diabetic smokers and non-smokers. METHODS:Patients were divided into 2 groups: (a) Group-1: type-2 ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2016.11.005
更新日期：2017-03-01 00:00:00
abstract：:Photodynamic therapy (PDT) is a promising modality for the treatment of cancer. PDT involves administering a photosensitizing dye, i.e. photosensitizer, that selectively accumulates in tumors, and shining a light source on the lesion with a wavelength matching the absorption spectrum of the photosensitizer, that exert...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章,评审
doi：10.1016/j.pdpdt.2019.04.033
更新日期：2019-06-01 00:00:00
abstract：AIM:Metronomic photodynamic therapy (mPDT) with a longer irradiation time and lower energy compared with acute (or classic) photodynamic therapy (aPDT) is a more effective treatment than aPDT for tumor cells, especially colorectal cancer. However, the underlying mechanisms of the superior effects of mPDT are unknown. ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.101809
更新日期：2020-09-01 00:00:00
abstract：BACKGROUND:Photodynamic therapy (PDT) is a well-known and effective treatment for non-melanoma skin-cancer. Numerous studies have also shown its effectiveness in mycosis fungoides. The aim of the study was to analyse MF patients treated with PDT at the Dermatology Unit of Bologna University. METHODS:We retrospectively...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2017.09.004
更新日期：2017-12-01 00:00:00
