Abstract：
AIM:Metronomic photodynamic therapy (mPDT) with a longer irradiation time and lower energy compared with acute (or classic) photodynamic therapy (aPDT) is a more effective treatment than aPDT for tumor cells, especially colorectal cancer. However, the underlying mechanisms of the superior effects of mPDT are unknown. METHODS:we used SWATH-MS (sequential window acquisition of all theoretical mass spectra) to identify differentially expressed proteins (DEPs) specific to aPDT (conventional fluence rate, 20 mW/cm2, 4 min 10 s), mPDT (metronomic fluence rate, 0.4 mW/cm2, 3.5 h), and control groups of SW837 cells. The photosensitizer used in both PDT methods was aminolevulinic acid which were incubated with the cells before irradiation. RESULTS:A total of 6805 proteins were identified in the three groups of SW837 cells. aPDT induced 333 DEPs and mPDT induced 1716 DEPs compared with the control. We identified 185 common DEPs in the two PDT groups, 148 different DEPs in the aPDT group, and 1531 different DEPs in the mPDT group. Most of the 185 common DEPs were involved in the extracellular component, participated in the processes of vesicle transport and secretion, binding, and hydrolase/catalytic activity. They were also involved in PI3K-Akt, cGMP-PKG, RAS, and aAMP signaling pathways. In addition, the 1531 different DEPs in the mPDT group participated in similar processes and molecular functions, but in a more complex manner than those in the aPDT group. CONCLUSION:our proteome data suggest that mPDT has a complex tumor destruction mechanism with more involved proteins compared with aPDT, which may explain the better tumor killing effect of mPDT.
journal_name
Photodiagnosis Photodyn Therjournal_title
Photodiagnosis and photodynamic therapyauthors
Gao H,Shi X,Chen Q,Che B,Yin H,Li Ydoi
10.1016/j.pdpdt.2020.101809subject
Has Abstractpub_date
2020-09-01 00:00:00pages
101809eissn
1572-1000issn
1873-1597pii
S1572-1000(20)30163-0journal_volume
31pub_type
杂志文章abstract：BACKGROUND:an accurate measurement of anterior chamber depth (ACD) both in eyes that underwent corneal refractive surgery (CRS) and in eyes that did not, can be crucial, as in case of intraocular lens (IOL) calculation in eyes that need to undergo cataract extraction, or during the evaluation of glaucomatous patients. ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.101976
更新日期：2020-12-01 00:00:00
abstract：:One of the factors limiting photodynamic therapy (PDT) is hypoxia in tumor cells during photodynamic action. PDT with pulse mode irradiation and appropriate irradiation parameters could be more effective in the singlet oxygen generation and tissue re-oxygenation than continuous wave (CW) mode. We theoretically demonst...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2016.01.003
更新日期：2016-03-01 00:00:00
abstract：BACKGROUND:Peritoneal carcinomatosis results when tumour cells implant and grow within the peritoneal cavity. Treatment and prognosis vary based on the primary cancer. Although therapy with intention-to-cure is offered to selective patients using cytoreductive surgery with chemotherapy, the prognosis remains poor for m...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章,评审
doi：10.1016/j.pdpdt.2017.10.021
更新日期：2017-12-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:To compare a single treatment of PDL-PDT with PDT for BCCs in terms of efficacy, aesthetic outcome, and pain in patients with multiple BCCs. STUDY DESIGN/MATERIALS AND METHODS:A prospective, controlled, intra-individual, investigator-blinded study was conducted on 15 patients with 62 BCCs. The...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2016.08.003
更新日期：2016-12-01 00:00:00
abstract：BACKGROUND:Conventional cancer treatment methods suffer from many limitations such as non-specificity in discrimination between healthy and malignant cells. The aim of this study was to investigate the role of polymeric gold-photoactive nanoparticles (PGPNPs) conjugated with folic acid (FA) as theranostic nanoparticles...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2019.101602
更新日期：2020-03-01 00:00:00
abstract：:Molecular modification of protoporphyrin IX (PpIX) was conducted to improve its water solubility and therapeutic performance for photodynamic therapy. The carboxylic acid and the two nitrogen atoms in the core of PpIX molecule were protonated following by conjugation with 3-aminopropyl triethoxysilane (APTES). Then, f...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2015.01.003
更新日期：2015-06-01 00:00:00
abstract：:Head and neck (H&N) cancer patients have a high incidence of local field change as well as second primary lung tumours. We have applied the Wolf Diagnostic Autofluorescence Endoscopy (DAFE) system in a novel way, combining autofluorescence evaluation of both H&N region and bronchial. New H&N cases as well as "old" cas...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2006.07.004
更新日期：2006-12-01 00:00:00
abstract：AIM:To compare the effectiveness of two protocols in the treatment of peri-implant mucositis (p-iM) among cigarette smokers. MATERIALS AND METHODS:Three groups were randomized into (i) single session of antimicrobial photodynamic therapy (aPDT) with mechanical debridement (MD) (Group-A), (ii) systemic AB with MD (Grou...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.pdpdt.2019.101587
更新日期：2020-03-01 00:00:00
abstract：:Basal cell carcinoma is one of the most common neoplasmatic diseases of the skin. Oral leukoplakia is precancerous stage. Early diagnosis of both diseases gives very good treatment results. Fluorescence spectroscopy is a new modality of diagnostics using excitation of absorbed PPIX in tumor. Spectroscopy is an objecti...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2008.10.001
更新日期：2008-12-01 00:00:00
abstract：:Antimicrobial photodynamic therapy uses a nontoxic photosensitizer with the assistance of harmless visible light to activate the photosensitizer. Consequently, the excited state of the photosensitizer interacts with molecular oxygen to produce reactive oxygen species, which have the antimicrobial effect. In this study...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2018.09.018
更新日期：2018-12-01 00:00:00
abstract：:Hidradenitis suppurativa (HS) is a chronic, inflammatory, and recurrent skin disease. Surgical excision of wounds appears to be the only curative treatment for the prevention of recurrence of moderate to severe stages. Magnetic resonance imaging (MRI) is a standard reference examination for the detection of HS peri-an...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2018.05.007
更新日期：2018-09-01 00:00:00
abstract：:We present the successful use of interstitial photodynamic therapy in the treatment of lacrimal gland mantle cell lymphoma associated with cervical and axillary lymphadenopathy in a 59-year-old female. ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2009.12.003
更新日期：2010-06-01 00:00:00
abstract：BACKGROUND:Optical coherence tomography (OCT) is a real-time non-invasive imaging tool, introduced in dermatology in the late 1990s. OCT uses near-infrared light impulses to produce images which can be displayed in cross-sectional and en-face mode. The technique has been used to image skin diseases especially non-melan...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章,评审
doi：10.1016/j.pdpdt.2017.02.003
更新日期：2017-06-01 00:00:00
abstract：BACKGROUND:Photodynamic therapy (PDT) is a well-known and effective treatment for non-melanoma skin-cancer. Numerous studies have also shown its effectiveness in mycosis fungoides. The aim of the study was to analyse MF patients treated with PDT at the Dermatology Unit of Bologna University. METHODS:We retrospectively...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2017.09.004
更新日期：2017-12-01 00:00:00
abstract：BACKGROUND:Nanosecond pulsed electric field (nsPEF) treatment is a new anti-cancer strategy with ultrashort pulse duration and high intensity of electric fields. The application of nsPEFs affects all intra- and extracellular membranes and independently initiates the process of apoptosis within cancer cells, leading the...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2015.11.002
更新日期：2015-12-01 00:00:00
abstract：BACKGROUND AND OBJECTIVES:This study aimed to compare the efficacy of fluorescein angiography (FA)-guided and indocyanine green angiography (ICGA)-guided half-time photodynamic therapy (PDT) for central serous chorioretinopathy (CSC). SUBJECTS AND METHODS:Medical records of 61 eyes of 61 CSC patients who underwent hal...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.101955
更新日期：2020-09-01 00:00:00
abstract：BACKGROUND:The objective of this paper was to verify if the oral administration of δ-aminolevulinic acid (ALA) in animals with prostate tumor can increase the sensitivity of cancer diagnosis by protoporphyrin IX blood autofluorescence. In this study, the autofluorescence of blood porphyrin was analyzed using fluorescen...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2010.12.006
更新日期：2011-03-01 00:00:00
abstract：INTRODUCTION:The aim of this study is to evaluate the antimicrobial activity of hypochlorite solutions and reciprocating instrumentation associated with photodynamic therapy (PDT). METHODS:One hundred and thirty two root canals were enlarged up to #35 K-file and inoculated with E.faecalis for 14 days. The 132 samples ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2018.07.015
更新日期：2018-09-01 00:00:00
abstract：:Folic acid functionalized carbon quantum dots (FA-CQDs) with ultrahigh quantum yield (50 %) were synthesized by one-pot hydrothermal route using citric acid. The synthesized CQDs were characterized by fluorescence spectroscopy, transmission electron microscopy (TEM), dynamic light scattering (DLS) and X-ray diffractio...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.101687
更新日期：2020-06-01 00:00:00
abstract：:Condyloma acuminata are a type of verrucous hyperplasia in the genital and perianal areas caused by human papilloma virus (HPV) infection. Traditional treatment methods for condyloma acuminata, such as topical medications, carbon dioxide (CO2) laser, cryotherapy, and surgical excision, can be effective at removing war...
journal_title：Photodiagnosis and photodynamic therapy
pub_type：
doi：10.1016/j.pdpdt.2020.101658
更新日期：2020-06-01 00:00:00
abstract：BACKGROUND:Recently, photodynamic diagnosis using 5-aminolevulinic acid (5-ALA) has gained attention for the diagnosis of neoplastic diseases. In the present study, anin vitro method of photodynamic cytodiagnosis (PDCD) performed using the reagent 5-ALA in the cytodiagnosis of solid pancreatic tumors was developed. Her...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2019.101581
更新日期：2019-10-16 00:00:00
abstract：:Lichen sclerosus et atrophicus (LSA) is disease of skin and mucosa, its pathogenesis remains unknown. Itching, pain and burning sensations and atrophy of vulva impair quality of life. Treatment is symptomatic. We report case of 30-year old woman with lesions in vulva in which series of topical PDT were carried out. We...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2012.02.002
更新日期：2012-06-01 00:00:00
abstract：BACKGROUND:Optical fibres can be placed in balloons to deliver light for intracranial photodynamic therapies. METHOD:A series of fibres, with either cleaved ends or terminating in cylindrical diffusers, housed in two different balloons, containing various solutions were investigated. The aim of the experiments perform...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2007.06.003
更新日期：2007-09-01 00:00:00
abstract：BACKGROUND:Active recruitment of inflammatory cells into tumors may be vital for antitumor immunity in cutaneous squamous cell carcinoma (cSCC) after photodynamic therapy. Chemokines play important roles in inflammatory cell recruitment. Moreover, C-X-C motif chemokine ligand 13 (CXCL13) is thought to be a pivotal chem...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2019.04.018
更新日期：2019-06-01 00:00:00
abstract：:Circumscribed choroidal hemangioma (CCH) is rarely associated with choroidal neovascularization (CNV), especially in HIV-infected patients. And the therapy is still controversial. In this study, we discussed the mechanism, clinical feature and treatment of such lesions, based on multi-modality imaging evidences. Photo...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2014.04.005
更新日期：2014-09-01 00:00:00
abstract：BACKGROUND:Optical coherence tomography (OCT) is a useful tool for the evaluation of structure and function of the kidney, but the image quality can be effected by many factors. OBJECTIVE:The objective of this study was to assess the image quality of different OCT systems in OCT imaging of the living kidney. METHODS:...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.101983
更新日期：2020-12-01 00:00:00
abstract：:Photophysical and in vitro photocytotoxicity studies were performed for the photosensitizer Dimegine, a disodium salt 2.4-di(alpha-methoxyethyl)-deuteroporphyrin-IX with very low systemic toxicity. The singlet oxygen and luminescence quantum yield were ΦΔ = 0,65 ± 0,06, and Φƒ=0,11 ± 0,01 respectively, and were indepe...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2018.11.006
更新日期：2019-03-01 00:00:00
abstract：BACKGROUND:Superluminescent diodes (sLED) appear to be an innovative and promising light source in photodynamic therapy (PDT), especially in actinic keratosis (AK) lesions treatment. AIM:Assessment of tolerability and efficacy of sLED in topical 5-aminolevulinic acid (ALA) PDT of AK lesions. METHODS:27 patients recei...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2018.04.010
更新日期：2018-06-01 00:00:00
abstract：BACKGROUND:Epithelial non-melanoma skin cancer (NMSC) like actinic keratosis (AK), Bowen's disease (BD) and basal cell carcinoma (BCC) represent the most common malignancies in the fair skinned population. Epidemiological data reveal high incidences, especially for actinic keratoses, which are basically non-invasive sq...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章,评审
doi：10.1016/j.pdpdt.2009.12.004
更新日期：2010-03-01 00:00:00
abstract：BACKGROUND:Colorectal cancer is one of the most common gastrointestinal malignancies. Photodynamic therapy (PDT) is a novel and non-invasive treatment for multiple tumors with its features of small trauma, good applicability, accurate targeting, etc. PDT is also a potential treatment for colon cancer that induces lots ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.102143
更新日期：2020-12-08 00:00:00
