Transcriptome analysis revealed pivotal transporters involved in the reduction of cadmium accumulation in pak choi (Brassica chinensis L.) by exogenous hydrogen-rich water.

Abstract:

:Hydrogen-rich water (HRW) has been widely used in research on plant resistance to Cd. However, the underlying molecular mechanism of HRW in ameliorating cadmium stress in vegetables is largely unknown. In this study, the RNA-sequencing analyses were used to characterize the role of HRW in the alleviation of Cd toxicity in Chinese cabbage seedlings. Based on the obtained results, two genes encoding metal ionic transporters, BcIRT1 and BcZIP2 were ultimately selected out. Then, a systematic validation of the metal ion transport function of these two ZIP-encoding genes of pak choi were performed via a yeast transformation system. The results showed that BcIRT1 and BcZIP2 increased the sensitivity of different yeast mutant strains to relative metal ionic stresses and facilitated the accumulation of metal ions (Cd2+, Mn2+, Zn2+, and Fe2+) in yeast; thus, it suggests that BcIRT1 and BcZIP2 probably have the ability to transport Cd2+, Mn2+, Zn2+ and Fe2+ in pak choi. The time-course and concentration-dependent expression profiles of BcIRT1 and BcZIP2 showed that as time with HRW increased, the effectiveness of the repression on the expression of BcIRT1 and BcZIP2 increased, and as the seedlings were exposed to increased Cd concentrations, the inhibition of BcIRT1 and BcZIP2 by HRW was also increased. Over all, these findings provide new insights into the genome-wide transcriptome profiles in pak choi and show that HRW reduced Cd uptake probably through inhibiting the expression of transporters related to Cd absorption, BcIRT1 and BcZIP2.

journal_name

Chemosphere

journal_title

Chemosphere

authors

Wu X,Zhu ZB,Chen JH,Huang YF,Liu ZL,Zou JW,Chen YH,Su NN,Cui J

doi

10.1016/j.chemosphere.2018.10.152

subject

Has Abstract

pub_date

2019-02-01 00:00:00

pages

684-697

eissn

0045-6535

issn

1879-1298

pii

S0045-6535(18)32014-9

journal_volume

216

pub_type

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