Determination by chromatography and cytotoxotoxic and oxidative effects of pyriproxyfen and pyridalyl.

Abstract:

:Pyriproxyfen (PPF) is a larvicide, used to combat the proliferation of Aedes aegypti larvae. The objective of this study was to analyze the compounds of pyriproxyfen and pyridalyl (PYL) in a commercial larvicide to analyze the cytotoxic and oxidative effects of PPF and PYL. The toxic potential of PPF and PYL were assessed based on lethal concentration (LC50) in Artemia salina, cytotoxicity based on the mitotic index and the chromosomal alterations in Allium cepa and the oxidative damage in Saccharomyces cerevisiae. The PPF and PYL compounds were identified by HPLC-PDA based on their retention times and spectral data. The wavelengths λmax (258 nm) and (271 nm) of the UV spectrum of PYL and PPF and the retention times (RT) (3.38 min) and (4.03 min), respectively. The toxicological potentials of PPF and PYL were significant at concentrations (1, 10, 100 and 1000 ppm), with an LC50 of 48 h (0.5 ppm). PPF and PYL pointed out a cytotoxic effect in A. cepa at all concentrations (0.0001, 0.001, 0.01, 0.1, 1.0, 100 and 1000 ppm), genotoxic effect at concentrations only (0.0001; 0.1; 1; 100 and 1000 ppm), and mutagenic for concentrations (0.1, 100 and 1000 ppm). In relation S. cerevisiae, PPF e PYL prompted oxidative damage at concentrations (100 and 1000 ppm) in all strains (SODWT, Sod1, Sod2, Sod1Sod2, Cat1 and Sod1Cat1). Therefore, the PPF and PYL identificated in commercial larvicide by HPLC-PDA produced cytotoxic and oxidative effects that could cause health and ecosystem risks.

journal_name

Chemosphere

journal_title

Chemosphere

authors

Alves PES,Oliveira MDDA,Marcos de Almeida P,Martins FA,Amélia de Carvalho Melo Cavalcante A,de Jesus Aguiar Dos Santos Andrade T,Feitosa CM,Rai M,Campinho Dos Reis A,Soares da Costa Júnior J

doi

10.1016/j.chemosphere.2019.02.037

subject

Has Abstract

pub_date

2019-06-01 00:00:00

pages

398-406

eissn

0045-6535

issn

1879-1298

pii

S0045-6535(19)30253-X

journal_volume

224

pub_type

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