Abstract：
:We present a new module integrated into the widely adopted PatternLab for proteomics to enable analysis of isotope-labeled peptides produced using dimethyl or SILAC. The accurate quantitation of proteins lies within the heart of proteomics; dimethylation has shown to be reliable, inexpensive, and applicable to any sample type. We validate our algorithm using an M. tuberculosis dataset obtained from two biological conditions; we used three dimethyl labels, one serving as an internal control for labeling a mixture of samples from both biological conditions. This internal control certified the proper functioning of our software. Availability: http://patternlabforproteomics.org, freely available for academic use.
journal_name
J Proteomicsjournal_title
Journal of proteomicsauthors
Santos MDM,Lima DB,Silva ARF,Kurt LU,Clasen MA,Pinto AFM,Moresco JJ,Yates JR 3rd,Aquino P,Barbosa VC,Fischer JSG,Carvalho PCdoi
10.1016/j.jprot.2019.04.021subject
Has Abstractpub_date
2019-06-30 00:00:00pages
103371eissn
1874-3919issn
1876-7737pii
S1874-3919(19)30134-4journal_volume
202pub_type
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