Abstract：
:In the present work we have revisited the application of quantitative ultrasound imaging (QUI) to cellular hydrogels, by using the reference phantom method (RPM) in combination with a local attenuation compensation algorithm. The investigated biological samples consisted of cell-laden collagen hydrogels with PC12 neural cells. These cell-laden hydrogels were used to calibrate the integrated backscattering coefficient (IBC) as a function of cell density, which was then used to generate parametric images of local cell density. The image resolution used for QUI and its impact on the relative IBC error was also investigated. Another important contribution of our work was the monitoring of PC12 cell proliferation. The cell number estimates obtained via the calibrated IBC compared well with data obtained using a conventional quantitative method, the MTS assay. Evaluation of spectral changes as a function of culture time also provided additional information on the cell cluster size, which was found to be in close agreement with that observed by microscopy. Last but not least, we also applied QUI on a 3D printed cellular construct in order to illustrate its capabilities for the evaluation of bioprinted structures. STATEMENT OF SIGNIFICANCE: While there is intensive research in the areas of polymer science, biology, and 3D bio-printing, there exists a gap in available characterisation tools for the non-destructive inspection of biological constructs in the three-dimensional domain, on the macroscopic scale, and with fast data acquisition times. Quantitative ultrasound imaging is a suitable characterization technique for providing essential information on the development of tissue engineered constructs. These results provide a detailed and comprehensive guide on the capabilities and limitations of the technique.
journal_name
Acta Biomaterjournal_title
Acta biomaterialiaauthors
Ruland A,Gilmore KJ,Daikuara LY,Fay CD,Yue Z,Wallace GGdoi
10.1016/j.actbio.2019.04.055subject
Has Abstractpub_date
2019-06-01 00:00:00pages
173-185eissn
1742-7061issn
1878-7568pii
S1742-7061(19)30298-3journal_volume
91pub_type
杂志文章abstract：:The objective of our study was to evaluate the influence of prolonged artificial ageing on oxidation resistance and the subsequent wear behaviour of vitamin E-stabilized, in comparison to standard and highly cross-linked remelted polyethylene (XLPE), and the degradation effect of third-body particles on highly cross-l...
journal_title：Acta biomaterialia
pub_type： 杂志文章
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abstract：:Spatiotemporally controlled release of growth factors (GFs) is critical for regenerative processes such as angiogenesis. A common strategy is to encapsulate the GF within hydrogels, with release being controlled via diffusion and/or gel degradation (i.e., hydrolysis and/or proteolysis). However, simple encapsulation s...
journal_title：Acta biomaterialia
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更新日期：2016-12-01 00:00:00
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journal_title：Acta biomaterialia
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journal_title：Acta biomaterialia
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abstract：:Blood-contacting medical devices are an integral part of modern medicine. Such devices may be used for only a few hours or may be implanted for life. Despite advances in biomaterial science, clotting on medical devices remains a common problem. Systemic administration of antiplatelet drugs or anticoagulants is often n...
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pub_type： 杂志文章,评审
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2018.02.026
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2013.05.001
更新日期：2013-08-01 00:00:00
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pub_type： 杂志文章
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journal_title：Acta biomaterialia
pub_type： 杂志文章,评审
doi：10.1016/j.actbio.2015.03.025
更新日期：2015-07-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2008.04.012
更新日期：2008-09-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2012.11.010
更新日期：2013-03-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2013.07.039
更新日期：2013-12-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.05.004
更新日期：2009-09-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2011.09.033
更新日期：2012-02-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2018.12.001
更新日期：2019-01-15 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2007.10.010
更新日期：2008-05-01 00:00:00
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journal_title：Acta biomaterialia
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journal_title：Acta biomaterialia
pub_type： 杂志文章,评审
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更新日期：2018-06-01 00:00:00
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journal_title：Acta biomaterialia
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更新日期：2021-02-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
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更新日期：2013-04-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2017.01.052
更新日期：2017-03-15 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
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更新日期：2020-03-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2018.04.022
更新日期：2018-06-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2019.04.023
更新日期：2019-06-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.08.052
更新日期：2016-11-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2014.05.025
更新日期：2014-10-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
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