Abstract:
:Transcutaneous immunization is the administration of a vaccine on the skin to generate efficient systemic and mucosal immune responses against an antigen. In the present study, reconstituted hepatitis B surface antigen vesicles (HBsAg-REVs) integrated with monophosphoryl lipid A were prepared by the delipidation-reconstitution method and tested as built-in adjuvanted vaccine, system for transcutaneous immunization using a combined approach of tape strippings, and enhanced antigen skin contact time. Prepared vesicles were extensively characterized for size, shape, zeta potential, and antigen protein loading efficiency. Following topical application, HBsAg-REVs skin permeation on isolated rat skin and cell uptake by bone marrow-derived dendritic cells were determined by confocal laser scanning microscopy and flow cytometry, respectively. The humoral and cellular immune responses elicited by HBsAg-REVs via transcutaneous immunization were comparable to the marketed intramuscular hepatitis B vaccine formulation with predefined immunization protocols. This study supports that delivery of reconstituted HBsAg vesicles via transcutaneous route may open a new vista for designing topical vaccines with possible immune protection against hepatitis B in future.
journal_name
J Pharm Scijournal_title
Journal of pharmaceutical sciencesauthors
Kaurav M,Kumar R,Jain A,Pandey RSdoi
10.1016/j.xphs.2019.07.007subject
Has Abstractpub_date
2019-11-01 00:00:00pages
3550-3559issue
11eissn
0022-3549issn
1520-6017pii
S0022-3549(19)30442-3journal_volume
108pub_type
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journal_title:Journal of pharmaceutical sciences
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