Abstract：
BACKGROUND:Recently, photodynamic diagnosis using 5-aminolevulinic acid (5-ALA) has gained attention for the diagnosis of neoplastic diseases. In the present study, anin vitro method of photodynamic cytodiagnosis (PDCD) performed using the reagent 5-ALA in the cytodiagnosis of solid pancreatic tumors was developed. Here, we assess the accuracy of PDCD for malignancy. MATERIALS AND METHODS:EUS-FNA was performed from September 2015 to March 2018 in patients with solid pancreatic tumors at Osaka Rosai Hospital. Samples were diagnosed independently by an expert pathologist and a medical doctor with conventional cytology and PDCD. RESULTS:A total of 53 patients (35 males, average age: 70.2 years old) were enrolled. The definitive diagnoses were 7 benign lesions and 46 malignant lesions. Using thein vitro PDCD method, the detection of reddish fluorescence in cell samples indicated cancer cells. PDCD had a sensitivity of 91.3% (42/46) and a specificity of 100% (7/7), while conventional cytology had a sensitivity of 93.5% (43/46) and a specificity of 85.7% (6/7). Two patients were successfully diagnosed with malignancy only by the PDCD method. CONCLUSIONS:In vitro PDCD performed using the 5-ALA method can effectively and safely identify a diagnosis of pancreatic cancer without requiring an expert pathologist. The sensitivity of this technique could be increased in the diagnosis of pancreatic malignancy by combining it with the conventional method.
journal_name
Photodiagnosis Photodyn Therjournal_title
Photodiagnosis and photodynamic therapyauthors
Hirao M,Hosui A,Mimura A,Tanimoto T,Onishi K,Kusumoto Y,Yamaguchi T,Yamada T,Miwa H,Hiramatsu Ndoi
10.1016/j.pdpdt.2019.101581subject
Has Abstractpub_date
2019-10-16 00:00:00pages
101581eissn
1572-1000issn
1873-1597pii
S1572-1000(19)30432-6pub_type
杂志文章abstract：BACKGROUND:Photodynamic diagnosis (PDD) as well as thermovision belong to the category of non-invasive optical diagnosis techniques. Among many different skin cancer diseases, basal cell carcinoma (BCC) is the most frequently occurring one (almost 95% of all skin tumours). In contrast, seborrhoeic keratosis represents ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2012.07.001
更新日期：2013-02-01 00:00:00
abstract：:Photodynamic therapy (PDT) has emerged as an effective and minimally invasive treatment option for several diseases, including some forms of cancer. However, several drawbacks of the approved photosensitizers (PS), such as insufficient light absorption at therapeutically relevant wavelengths hampered the clinical effe...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章,评审
doi：10.1016/j.pdpdt.2015.07.001
更新日期：2016-03-01 00:00:00
abstract：:In this review article we described the applications of various nanoparticles that can be used for photodynamic therapy (PDT), such as: plasmonic nanoparticles, quantum dots and upconversion nanoparticles. In comparison with typical organic photosensitizers such as hematoporphyrins, they exhibit higher photostability ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章,评审
doi：10.1016/j.pdpdt.2019.03.016
更新日期：2019-06-01 00:00:00
abstract：BACKGROUND:Epithelial non-melanoma skin cancer (NMSC) like actinic keratosis (AK), Bowen's disease (BD) and basal cell carcinoma (BCC) represent the most common malignancies in the fair skinned population. Epidemiological data reveal high incidences, especially for actinic keratoses, which are basically non-invasive sq...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章,评审
doi：10.1016/j.pdpdt.2009.12.004
更新日期：2010-03-01 00:00:00
abstract：BACKGROUND:Although aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) photodynamic therapy (PDT) is an effective FDA-approved therapy for actinic keratosis (AK), a substantial fraction of patients (up to 25%) do not respond to treatment. This study examined the feasibility of using pre-treatment measurements o...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 临床试验,杂志文章
doi：10.1016/j.pdpdt.2015.10.006
更新日期：2015-12-01 00:00:00
abstract：BACKGROUND:Antibacterial photodynamic therapy (aPDT) has been proposed as an alternative strategy to inactivate bacteria. This study was designed to investigate the antibacterial effect of a novel photosensitizer S-Porphin sodium (S-PS) on plankton and biofilm cultures of Staphylococcus aureus (S. aureus) and its multi...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2019.08.031
更新日期：2019-12-01 00:00:00
abstract：INTRODUCTION:Clinical studies have shown the usefulness of antimicrobial photodynamic therapy (aPDT) as an adjunctive in periodontal therapy. These studies did not utilize indocyanine green (ICG) as a recently introduced photosensitizer. The aim of this study was to perform a full-mouth double-blind randomized controll...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/j.pdpdt.2016.02.007
更新日期：2016-06-01 00:00:00
abstract：BACKGROUND:Microorganisms treated by photo-activated disinfection (PAD) in combined periodontal-endodontic (perio-endo) lesions would be exposed to sub-lethal doses of PAD (sPAD). This study evaluated the effect of sPAD using toluidine blue O (TBO) in combination with diode laser irradiation on the growth and biofilm-f...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2016.08.013
更新日期：2016-12-01 00:00:00
abstract：BACKGROUND:The development of nanocarriers is an important approach to increase the bioavailability of hydrophilic drugs in target cells. In this work, we evaluated the anti-tumorigenic mechanisms and efficacy of NanoALA, a novel nanoformulation of aminolevulic acid (ALA) based on poly(lactide-co-glycolide) (PLGA) nano...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2017.08.011
更新日期：2017-12-01 00:00:00
abstract：BACKGROUND:Previous in vivo studies on photodynamic therapy (PDT)-treated, high cellular density tumors showed evidences of a bystander effect accompanying the therapy, cellular death continuing beyond the limits of the photochemical reactions in time and space. This process is generated by the initially damaged cells ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2012.03.001
更新日期：2012-12-01 00:00:00
abstract：BACKGROUND:Active recruitment of inflammatory cells into tumors may be vital for antitumor immunity in cutaneous squamous cell carcinoma (cSCC) after photodynamic therapy. Chemokines play important roles in inflammatory cell recruitment. Moreover, C-X-C motif chemokine ligand 13 (CXCL13) is thought to be a pivotal chem...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2019.04.018
更新日期：2019-06-01 00:00:00
abstract：OBJECTIVE:Evaluate the efficacy of 5-Aminolevulinic Acid Photodynamic Therapy (PDT) in medical treatment of nasal inverted papilloma (NIP). METHODS:Three patients with nasal inverted papilloma were treated with 5-Aminolevulinic Acid Photodynamic Therapy at our department from April to September 2012. The efficacy and ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2013.05.010
更新日期：2013-12-01 00:00:00
abstract：:A 35-year-old woman with a history of pulp canal obliteration (PCO) and apical periodontitis was treated with guided endodontics combined with antimicrobial photodynamic therapy (aPDT). The use of aPDT was paramount to the approach performed, and its application should be encouraged in cases of severely calcified cana...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.101935
更新日期：2020-09-01 00:00:00
abstract：AIM:The current clinical trial aimed to assess the effectiveness of adjunctive photodynamic therapy (aPDT) and adjunctive antibiotic gel therapy (aAGT) to treat peri-implantitis among patients with type 2 diabetes mellitus (T2DM). METHODS:Selected T2DM participants with peri-implantitis were distributed into 3 groups:...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.102077
更新日期：2020-12-01 00:00:00
abstract：OBJECTIVES:The purpose of this study was to investigate the effects of 5-aminolaevulinic acid mediated photodynamic therapy (ALA-PDT) on the survival activity and apoptosis of human melanoma cell line A375 and non-melanoma skin carcinoma cell line A431 cells. The mechanism for cellular apoptosis was explored. METHODS:...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2018.01.004
更新日期：2018-03-01 00:00:00
abstract：OBJECTIVE:The aim of the present 6-month follow-up study was to assess the effect of mechanical debridement (MD) with adjunct antimicrobial photodynamic therapy (aPDT) in the treatment of peri-implant diseases in type-2 diabetic smokers and non-smokers. METHODS:Patients were divided into 2 groups: (a) Group-1: type-2 ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2016.11.005
更新日期：2017-03-01 00:00:00
abstract：BACKGROUND:Photodynamic therapy has apart from a direct cytotoxic effect also immunomodulatory properties. The aim of our study was to investigate how photodynamic therapy with 5-aminolevulinic acid (ALA-PDT) in sublethal doses influences the secretion of interleukins 6, 8 and 10 from colon cancer cells in vitro. METH...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2018.02.021
更新日期：2018-06-01 00:00:00
abstract：BACKGROUND:Microbial keratitis is a potential cause of corneal blindness. We investigated the amoebicidal efficacy of photodynamic antimicrobial therapy with a light-emitting diode as the light source and the cationic chlorin derivative TONS504 as the photosensitizer for the elimination of Acanthamoeba, a causative org...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2019.08.035
更新日期：2019-12-01 00:00:00
abstract：:Photodynamic therapy (PDT) is a promising modality for the treatment of cancer. PDT involves administering a photosensitizing dye, i.e. photosensitizer, that selectively accumulates in tumors, and shining a light source on the lesion with a wavelength matching the absorption spectrum of the photosensitizer, that exert...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章,评审
doi：10.1016/j.pdpdt.2019.04.033
更新日期：2019-06-01 00:00:00
abstract：:We have more than 10 years experience in the Photobiology Unit, in the use of topical photodynamic therapy (PDT) for non-melanoma skin cancer and other skin diseases. During this time we have undertaken approximately 5000 treatments and this article details the practical aspects of how we treat Bowen's disease with to...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2009.04.001
更新日期：2009-03-01 00:00:00
abstract：:The tumor microenvironment (TME) affects not only tumor growth and metastasis, but also therapy efficacy. In the present study, an oxygen self-supplying delivery system for a photosensitizer (O2-PDS), with an oxygen source of calcium peroxide (CPO), has been designed to induce multi-path tumor apoptosis through intera...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2019.101642
更新日期：2020-03-01 00:00:00
abstract：BACKGROUND:The aim of this study was to evaluate the effect of multiple sessions of antimicrobial photodynamic therapy (aPDT) on the treatment of experimental periodontitis (EP) in rats treated with chemotherapy. METHODS:Chemotherapy using 5-fluorouracil (5-FU) consisted of intraperitoneal administration of 60 and 40 ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2018.11.014
更新日期：2019-03-01 00:00:00
abstract：INTRODUCTION:Photodynamic therapy (PDT) is a minimally invasive intervention used in the management of tissue disorders. In this retrospective study, a total of 62 patients with actinic keratosis (AKs) were treated with surface illumination PDT. Comparisons with the clinical features, rate of recurrence as well as mali...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2016.04.017
更新日期：2017-03-01 00:00:00
abstract：:Decreasing incubation time, as well as enhanced PpIX production, are present challenges for topical photodynamic therapy (PDT). There are reports concerning the existence of a strong relationship between site temperature and porphyrin synthesis in biological tissue, that suggest temperature increase in the tissue can ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.101786
更新日期：2020-06-01 00:00:00
abstract：BACKGROUND:Autofluorescence bronchoscopy (AFB) is a highly sensitive tool for the detection of early bronchial cancers. However, its specificity remains limited due to primarily false positive results induced by hyperplasia, metaplasia and inflammation. We have investigated the potential of blue-violet backscattered li...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2008.01.005
更新日期：2008-03-01 00:00:00
abstract：BACKGROUND:Nanosecond pulsed electric field (nsPEF) treatment is a new anti-cancer strategy with ultrashort pulse duration and high intensity of electric fields. The application of nsPEFs affects all intra- and extracellular membranes and independently initiates the process of apoptosis within cancer cells, leading the...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2015.11.002
更新日期：2015-12-01 00:00:00
abstract：AIM:Metronomic photodynamic therapy (mPDT) with a longer irradiation time and lower energy compared with acute (or classic) photodynamic therapy (aPDT) is a more effective treatment than aPDT for tumor cells, especially colorectal cancer. However, the underlying mechanisms of the superior effects of mPDT are unknown. ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.101809
更新日期：2020-09-01 00:00:00
abstract：:Photodynamic therapy is one of the hot topics in cancer studies. Photosensitizing chemical substrates are stimulated by light having a specific wavelength to cause fatal effect on different kinds of targets. In this study, axially 4-{[(1E)-2-furylmethylene]amino}phenol, 4-{[(1E)-2-thienylmethylene]amino}phenol and 4-{...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2018.05.009
更新日期：2018-09-01 00:00:00
abstract：BACKGROUND:Peritoneal carcinomatosis results when tumour cells implant and grow within the peritoneal cavity. Treatment and prognosis vary based on the primary cancer. Although therapy with intention-to-cure is offered to selective patients using cytoreductive surgery with chemotherapy, the prognosis remains poor for m...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章,评审
doi：10.1016/j.pdpdt.2017.10.021
更新日期：2017-12-01 00:00:00
abstract：:Hidradenitis Suppurativa (HS) is a distressing painful chronic inflammatory skin follicular condition. It is a challenge to achieve better therapeutic effect and lower recurrence rate. In this report, ALA-PDT combined with surgery for the treatment achieved ulcer healing, pain elimination, with no relapse during our f...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2019.07.024
更新日期：2019-12-01 00:00:00
