Abstract：
BACKGROUND:Microbial keratitis is a potential cause of corneal blindness. We investigated the amoebicidal efficacy of photodynamic antimicrobial therapy with a light-emitting diode as the light source and the cationic chlorin derivative TONS504 as the photosensitizer for the elimination of Acanthamoeba, a causative organism of corneal infection and blindness. Acanthamoeba keratitis remains a challenge to treat because of limited available treatments. METHODS:Acanthamoeba castellani 50370 was exposed to TONS504 at various concentrations (0, 1, or 10 mg/L for trophozoites; 0, 1, 10, or 20 mg/L for cysts), irradiated at various light energies (0, 10, or 30 J/cm2 for trophozoites; 0, 30, or 60 J/cm2 for cysts), and incubated at 26 °C for 3 h. Assessment of cell viability by trypan blue staining revealed that photodynamic antimicrobial therapy attenuated the survival of trophozoites and cysts dependent on TONS504 concentration and light energy. RESULTS:Photodynamic antimicrobial therapy with 10 mg/L TONS504 and 30 J/cm2 light energy suppressed trophozoite viability by 77%, and 20 mg/L TONS504 and 60 J/cm2 light energy attenuated cyst survival by 42%. Staining with fluorescein isothiocyanate-conjugated annexin V and ethidium homodimer III revealed photodynamic antimicrobial therapy induced apoptosis and necrosis in trophozoites dependent upon the intensity of treatment, whereas apoptosis was the predominant form of cell death in cysts. CONCLUSIONS:Photodynamic antimicrobial therapy with TONS504 warrants further investigation as a potential treatment modality for Acanthamoeba keratitis.
journal_name
Photodiagnosis Photodyn Therjournal_title
Photodiagnosis and photodynamic therapyauthors
Pertiwi YD,Chikama T,Sueoka K,Ko JA,Kiuchi Y,Onodera M,Sakaguchi Tdoi
10.1016/j.pdpdt.2019.08.035subject
Has Abstractpub_date
2019-12-01 00:00:00pages
166-171eissn
1572-1000issn
1873-1597pii
S1572-1000(19)30149-8journal_volume
28pub_type
杂志文章abstract：BACKGROUND:The Yorkshire Laser Centre team have been engaged in photodynamic therapy (PDT) since 1990. In this article we review our experience in bronchoscopic PDT for lung cancer and outline our current indications and results. METHODS:160 patients in 2 groups entered into a prospective study: Group A (N=144) were s...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/S1572-1000(04)00047-X
更新日期：2004-11-01 00:00:00
abstract：BACKGROUND:The development of nanocarriers is an important approach to increase the bioavailability of hydrophilic drugs in target cells. In this work, we evaluated the anti-tumorigenic mechanisms and efficacy of NanoALA, a novel nanoformulation of aminolevulic acid (ALA) based on poly(lactide-co-glycolide) (PLGA) nano...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2017.08.011
更新日期：2017-12-01 00:00:00
abstract：:In the present study, the in vitro susceptibilities of five Candida spp. to photodynamic antimicrobial chemotherapy (PACT) with four phenothiazinium derivatives, methylene blue (MB), new methylene blue N (NMBN), toluidine blue O (TBO) and the novel pentacyclic phenothiazinium photosensitiser S137, in combination with ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2012.11.004
更新日期：2013-05-01 00:00:00
abstract：OBJECTIVES:The purpose of this study was to investigate the effects of 5-aminolaevulinic acid mediated photodynamic therapy (ALA-PDT) on the survival activity and apoptosis of human melanoma cell line A375 and non-melanoma skin carcinoma cell line A431 cells. The mechanism for cellular apoptosis was explored. METHODS:...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2018.01.004
更新日期：2018-03-01 00:00:00
abstract：:Glucose is the main energy substance to drive the physiological events of the cell.. Malignant cells exhibit a much higher rate of glycolysis than healthy cells to relieve the increased needs of energy. The higher metabolic rate induces the over-expression of the Glucose Transporter (GLUT) to transport more glucose in...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.102014
更新日期：2020-12-01 00:00:00
abstract：BACKGROUND:The potential application of TOOKAD(®)-PDT for the treatment of blood vessels was investigated. TOOKAD(®) (WST09), a novel palladium-bacteriopheophorbide absorbs light in the near IR with a high quantum yield of intersystem crossing. Our study assessed the efficacy of this drug in inducing vascular damage wi...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/S1572-1000(05)00006-2
更新日期：2005-03-01 00:00:00
abstract：:Palmatine hydrochloride (PaH) is a natural active compound from a traditional Chinese medicine (TCM). The present study aims to evaluate the effect of PaH as a new photosensitizer on colon adenocarcinoma HT-29 cells upon light irradiation. Firstly, the absorption and fluorescence spectra of PaH were measured using a U...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2016.05.005
更新日期：2016-09-01 00:00:00
abstract：AIM:Metronomic photodynamic therapy (mPDT) with a longer irradiation time and lower energy compared with acute (or classic) photodynamic therapy (aPDT) is a more effective treatment than aPDT for tumor cells, especially colorectal cancer. However, the underlying mechanisms of the superior effects of mPDT are unknown. ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.101809
更新日期：2020-09-01 00:00:00
abstract：BACKGROUND AND PURPOSE:Breast cancer is one of the most widely recognized tumors. .Diagnosis made in the early stage of disease may imporve outcomes. The discovery of malignant growth utilizing noninvasive light intrusive methods in lieu of conventional excisional biopsy may assist in achieving this goal. MATERIALS AN...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.101922
更新日期：2020-09-01 00:00:00
abstract：SIGNIFICANCE:The estimation of tissue oxygenation is vital in the diagnosis and therapeutic evaluation of a huge assortment of diseases. However, the hyperspectral (HS) imaging system is a rising innovation that can be utilized to build a highly sensitive, non-invasive, and tissue hemoglobin immersion map. OBJECTIVE:A...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2021.102190
更新日期：2021-01-25 00:00:00
abstract：:Head and neck (H&N) cancer patients have a high incidence of local field change as well as second primary lung tumours. We have applied the Wolf Diagnostic Autofluorescence Endoscopy (DAFE) system in a novel way, combining autofluorescence evaluation of both H&N region and bronchial. New H&N cases as well as "old" cas...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2006.07.004
更新日期：2006-12-01 00:00:00
abstract：BACKGROUND:Photodynamic therapy is a promising invention to treat infections and cancer where conventional treatments are insufficient and have many side effects. Photodynamic therapy is mainly emphasized as having minimal side effects on healthy cells during local applications, even so photosensitizer can accumulate i...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.101891
更新日期：2020-09-01 00:00:00
abstract：BACKGROUND:To investigate the changes in retinal and choriocapillaris vessel density (VD) in patients affected by early age-related macular degeneration (AMD) and reticular pseudodrusen (RPD), using Optical Coherence Tomography Angiography (OCTA). METHODS:A total of seventy-eight eyes of 78 patients (43 male, 35 femal...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.102094
更新日期：2020-11-11 00:00:00
abstract：:Non-melanotic cutaneous cancers and melanoma are the main common type skin cancers worldwide. Despite many therapeutic options, therapeutic efficacy is not satisfied in all patients with advanced skin cancers, especially in melanoma. Photodynamic therapy (PDT) is a technology for skin disease treatment because of its ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2019.08.023
更新日期：2019-12-01 00:00:00
abstract：AIM:The aim of this study was to evaluate the effectiveness of final decontamination protocols against Enterococcus faecalis and their influence on bond strength of filling material to root canal dentin. MATERIAL AND METHODS:Ninety root canals were enlarged with ProTaper system and inoculated with E. faecalis for 15da...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2016.11.004
更新日期：2017-03-01 00:00:00
abstract：BACKGROUND:Long treatment periods to reach a substantial inactivation of microorganisms are one of the critical challenges in the photodynamic therapy field. METHODS:Planktonic suspensions of Streptococcus mutans were treated in different groups: presence of rose bengal (RB at 2μM) and light exposure by a new high pot...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2016.05.001
更新日期：2016-09-01 00:00:00
abstract：:Wounds constitute severe problems in public health. Inappropriate manipulation to promote wound healing and indiscriminate use of antibiotics may contribute to failure in wound treatment, leading to bacterial growth and resistance. Appropriate and correct approaches to wound treatment are crucially important. Further,...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.101682
更新日期：2020-06-01 00:00:00
abstract：:Photodynamic therapy (PDT) is a clinically approved therapeutic modality used for the management of several types of tumors as well as non-malignant diseases. Most of the effects of this treatment regimen result from direct action of singlet oxygen and reactive oxygen species. However, accumulating evidence indicates ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/S1572-1000(05)00098-0
更新日期：2005-12-01 00:00:00
abstract：:A 35-year-old woman with a history of pulp canal obliteration (PCO) and apical periodontitis was treated with guided endodontics combined with antimicrobial photodynamic therapy (aPDT). The use of aPDT was paramount to the approach performed, and its application should be encouraged in cases of severely calcified cana...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.101935
更新日期：2020-09-01 00:00:00
abstract：BACKGROUND:To explore the surface modification of dentin using Er,Cr:YSGG phototherapy and bonding system on the shear bond strength and microleakage scores at two different distances and times of laser irradiation. MATERIALS AND METHODS:Ninety disease-free third molars were collected for the experiment. The four lase...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2018.10.017
更新日期：2019-03-01 00:00:00
abstract：BACKGROUND:Optical fibres can be placed in balloons to deliver light for intracranial photodynamic therapies. METHOD:A series of fibres, with either cleaved ends or terminating in cylindrical diffusers, housed in two different balloons, containing various solutions were investigated. The aim of the experiments perform...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2007.06.003
更新日期：2007-09-01 00:00:00
abstract：:A case of recalcitrant dissecting cellulitis of the scalp (DCS) in a 23-year-old male patient was treated with 4 times of topical ALA-PDT at intervals of 10-15 days. The patient responded well without any unbearable adverse effects and presented satisfactory effects. Before applying this therapy, the patient and his d...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2018.10.001
更新日期：2018-12-01 00:00:00
abstract：OBJECTIVES:To investigate the effects of low-dose aminolevulinic acid photodynamic therapy (ALA-PDT) on photoaging in human dermal fibroblasts (HDFs) and to explore the mechanism of Nuclear factor erythroid 2-related factor 2(Nrf2)-mediated photorejuvenation in vitro. METHODS:A photoaging model was established through...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2019.05.006
更新日期：2019-09-01 00:00:00
abstract：INTRODUCTION:Clinical studies have shown the usefulness of antimicrobial photodynamic therapy (aPDT) as an adjunctive in periodontal therapy. These studies did not utilize indocyanine green (ICG) as a recently introduced photosensitizer. The aim of this study was to perform a full-mouth double-blind randomized controll...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/j.pdpdt.2016.02.007
更新日期：2016-06-01 00:00:00
abstract：:Condyloma acuminata are a type of verrucous hyperplasia in the genital and perianal areas caused by human papilloma virus (HPV) infection. Traditional treatment methods for condyloma acuminata, such as topical medications, carbon dioxide (CO2) laser, cryotherapy, and surgical excision, can be effective at removing war...
journal_title：Photodiagnosis and photodynamic therapy
pub_type：
doi：10.1016/j.pdpdt.2020.101658
更新日期：2020-06-01 00:00:00
abstract：OBJECTIVE:To evaluate the clinical efficacy and safety of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) combined with a carbon dioxide laser (CO2 laser + PDT) versus CO2 laser for the treatment of low-grade vaginal intraepithelial neoplasms (vaginal LSIL). METHODS:We recruited 40 patients with vaginal LSIL and ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.101731
更新日期：2020-06-01 00:00:00
abstract：BACKGROUND:Vaginal intraepithelial neoplasia (VAIN) has a high-risk for recurrence and may precede genital cancers, such as vaginal cancer and/or other invasive diseases. Human papillomavirus (HPV)-induced VAIN may occur after loop electrosurgical excision procedures (LEEPs) or panhysterectomy. 5-aminolevulinic acid (A...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.101837
更新日期：2020-09-01 00:00:00
abstract：BACKGROUND:Photodynamic diagnosis (PDD) as well as thermovision belong to the category of non-invasive optical diagnosis techniques. Among many different skin cancer diseases, basal cell carcinoma (BCC) is the most frequently occurring one (almost 95% of all skin tumours). In contrast, seborrhoeic keratosis represents ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2012.07.001
更新日期：2013-02-01 00:00:00
abstract：:The relationship between cytomegalovirus (CMV), a severe complication in immunocompromised patients, and graft-versus-host disease (GVHD) has already been reported but remains without complete elucidation, especially concerning oral lesions. Considering that antimicrobial photodynamic therapy (aPDT) has an antimicrobi...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.101849
更新日期：2020-12-01 00:00:00
abstract：BACKGROUND:Although triple antibiotic paste (TAP) has been successfully used as an intracanal medicament for regenerative endodontic treatments, TAP has also been shown to cause discolouration. The aim of this study is to investigate the efficacy of different bleaching agents to bleach teeth discoloured from TAP. METH...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.101901
更新日期：2020-09-01 00:00:00
