Abstract:
:Immunogenic cell death (ICD), induced by certain anticancer chemotherapeutics, leads to the emission of danger associated molecular patterns (DAMP) by cancer cells, which facilitates the attraction, activation and maturation of dendritic cells (DC) as well as the subsequent priming of effector T cells. In this context calreticulin (CALR) exposed at an early stage of ICD at the surface of the cancer cells serves as phagocytic signal and triggers the formation of immunological synapses between malignant cells and DC. Subsequent phagocytosis facilitates the transfer of tumor associated antigen and thus depicts a fundamental step in the generation of anticancer immunity. Here we provide an imaging flowcytometric protocol for the quantification of ICD-associated DC phagocytosis of cancer cells. As compared to the traditional flowcytometry-based analysis, the presented method offers additional means of differentiation between the transient formation of immunological synapses and the final DC-mediated phagocytosis of cancer cells.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Cerrato G,Liu P,Martins I,Kepp O,Kroemer Gdoi
10.1016/bs.mie.2019.07.021subject
Has Abstractpub_date
2020-01-01 00:00:00pages
27-37eissn
0076-6879issn
1557-7988pii
S0076-6879(19)30301-5journal_volume
632pub_type
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