Abstract：
:Living systems integrate biochemical reactions that determine the functional state of each cell. Reactions are primarily mediated by proteins. In proteomic studies, these have been treated as independent entities, disregarding their higher-level organization into complexes that affects their activity and/or function and is thus of great interest for biological research. Here, we describe the implementation of an integrated technique to quantify cell-state-specific changes in the physical arrangement of protein complexes concurrently for thousands of proteins and hundreds of complexes. Applying this technique to a comparison of human cells in interphase and mitosis, we provide a systematic overview of mitotic proteome reorganization. The results recall key hallmarks of mitotic complex remodeling and suggest a model of nuclear pore complex disassembly, which we validate by orthogonal methods. To support the interpretation of quantitative SEC-SWATH-MS datasets, we extend the software CCprofiler and provide an interactive exploration tool, SECexplorer-cc.
journal_name
Cell Systjournal_title
Cell systemsauthors
Heusel M,Frank M,Köhler M,Amon S,Frommelt F,Rosenberger G,Bludau I,Aulakh S,Linder MI,Liu Y,Collins BC,Gstaiger M,Kutay U,Aebersold Rdoi
10.1016/j.cels.2020.01.001subject
Has Abstractpub_date
2020-02-26 00:00:00pages
133-155.e6issue
2eissn
2405-4712issn
2405-4720pii
S2405-4712(20)30001-6journal_volume
10pub_type
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