Abstract：
:Temporal interference (TI) is a non-invasive neurostimulation technique that utilizes high-frequency external electric fields to stimulate deep neuronal structures without affecting superficial, off-target structures. TI represents a potential breakthrough for treating conditions, such as Parkinson's disease and chronic pain. However, early clinical work on TI stimulation was met with mixed outcomes challenging its fundamental mechanisms and applications. Here, we apply established physics to study the mechanisms of TI with the goal of optimizing it for clinical use. We argue that TI stimulation cannot work via passive membrane filtering, as previously hypothesized. Instead, TI stimulation requires an ion-channel mediated signal rectification process. Unfortunately, this mechanism is also responsible for high-frequency conduction block in off-target tissues, thus challenging clinical applications of TI. In consequence, we propose a set of experimental controls that should be performed in future experiments to refine our understanding and practice of TI stimulation. A record of this paper's transparent peer review process is included in the Supplemental Information.
journal_name
Cell Systjournal_title
Cell systemsauthors
Mirzakhalili E,Barra B,Capogrosso M,Lempka SFdoi
10.1016/j.cels.2020.10.004subject
Has Abstractpub_date
2020-12-16 00:00:00pages
557-572.e5issue
6eissn
2405-4712issn
2405-4720pii
S2405-4712(20)30372-0journal_volume
11pub_type
杂志文章相关文献
Cell Systems文献大全abstract：:Although genetic mutations that alter organisms' average lifespans have been identified in aging research, our understanding of the dynamic changes during aging remains limited. Here, we integrate single-cell imaging, microfluidics, and computational modeling to investigate phenotypic divergence and cellular heterogen...
journal_title：Cell systems
pub_type： 杂志文章
doi：10.1016/j.cels.2019.02.002
更新日期：2019-03-27 00:00:00
abstract：:Machine learning can be used to predict compounds acting synergistically, and this could greatly expand the universe of available potential treatments for diseases that are currently hidden in the dark chemical matter. ...
journal_title：Cell systems
pub_type： 杂志文章
doi：10.1016/j.cels.2015.12.005
更新日期：2015-12-23 00:00:00
abstract：:All mammals progress through similar physiological stages throughout life, from early development to puberty, aging, and death. Yet, the extent to which this conserved physiology reflects underlying genomic events is unclear. Here, we map the common methylation changes experienced by mammalian genomes as they age, foc...
journal_title：Cell systems
pub_type： 杂志文章
doi：10.1016/j.cels.2020.06.006
更新日期：2020-08-26 00:00:00
abstract：:Escherichia coli gene expression knockdown using synthetic small RNA (sRNA) can be fine-tuned by altering sRNA sequences to modulate target mRNA-binding ability, but this requires thorough checking for off-target effects. Here, we present an sRNA gene expression knockdown system fine-tuned by using different promoters...
journal_title：Cell systems
pub_type： 杂志文章
doi：10.1016/j.cels.2017.08.016
更新日期：2017-10-25 00:00:00
abstract：:How do rich biological behaviors arise from bi-molecular collisions? ...
journal_title：Cell systems
pub_type： 杂志文章
doi：10.1016/j.cels.2015.09.006
更新日期：2015-09-23 00:00:00
abstract：:One snapshot of the peer review process for "Death Rate of E. coli during Starvation Is Set by Maintenance Cost and Biomass Recycling" (Schink et al., 2019). ...
journal_title：Cell systems
pub_type： 评论,杂志文章
doi：10.1016/j.cels.2019.07.004
更新日期：2019-07-24 00:00:00
abstract：:Cell-to-cell variation in gene expression and the propagation of such variation (PoV or "noise propagation") from one gene to another in the gene network, as reflected by gene-gene correlation across single cells, are commonly observed in single-cell transcriptomic studies and can shape the phenotypic diversity of cel...
journal_title：Cell systems
pub_type： 杂志文章
doi：10.1016/j.cels.2017.03.002
更新日期：2017-04-26 00:00:00
abstract：:Here, we outline p-Creode, a new algorithm to construct multi-branching cell lineage trajectories from single-cell data. Application of this platform to diverse sources of single-cell data demonstrates its robustness and scalability, while the discovery of a new origin for rare gut tuft cells showcases the utility of ...
journal_title：Cell systems
pub_type： 评论,杂志文章
doi：10.1016/j.cels.2017.12.015
更新日期：2018-01-24 00:00:00
abstract：:While decades of research have elucidated many steps of the alphavirus lifecycle, the earliest replication dynamics have remained unclear. This missing time window has obscured early replicase strand-synthesis behavior and prevented elucidation of how the first events of infection might influence subsequent viral comp...
journal_title：Cell systems
pub_type： 杂志文章
doi：10.1016/j.cels.2020.12.005
更新日期：2021-01-21 00:00:00
abstract：:Medically induced loss of consciousness (mLOC) during anesthesia is associated with a macroscale breakdown of brain connectivity, yet the neural microcircuit correlates of mLOC remain unknown. To explore this, we applied different analytical approaches (t-SNE/watershed segmentation, affinity propagation clustering, PC...
journal_title：Cell systems
pub_type： 杂志文章
doi：10.1016/j.cels.2019.03.007
更新日期：2019-05-22 00:00:00
abstract：:Isogenic cells in a common environment show substantial cell-to-cell variation in gene expression, often referred to as "expression noise." Here, we use multiple single-cell RNA-sequencing datasets to identify features associated with high or low expression noise in mouse embryonic stem cells. These include the core p...
journal_title：Cell systems
pub_type： 杂志文章
doi：10.1016/j.cels.2017.10.003
更新日期：2017-11-22 00:00:00
abstract：:Understanding the complex interactions that occur between heterologous and native biochemical pathways represents a major challenge in metabolic engineering and synthetic biology. We present a workflow that integrates metabolomics, proteomics, and genome-scale models of Escherichia coli metabolism to study the effects...
journal_title：Cell systems
pub_type： 杂志文章
doi：10.1016/j.cels.2016.04.004
更新日期：2016-05-25 00:00:00
abstract：:Three studies demonstrate the potential of state-of-the-art mass spectrometry-based proteomics for rapid, deep characterization of proteomes. ...
journal_title：Cell systems
pub_type： 评论,杂志文章
doi：10.1016/j.cels.2016.03.002
更新日期：2016-03-23 00:00:00
abstract：:Two recent studies in Cell and Science demonstrate the reconstruction of global mechanistic networks and identification of regulatory principles from multi-omics data. ...
journal_title：Cell systems
pub_type： 评论,杂志文章
doi：10.1016/j.cels.2017.01.007
更新日期：2017-01-25 00:00:00
abstract：:The recent increase of immunopeptidomics data, obtained by mass spectrometry or binding assays, opens up possibilities for investigating endogenous antigen presentation by the highly polymorphic human leukocyte antigen class I (HLA-I) protein. State-of-the-art methods predict with high accuracy presentation by HLA all...
journal_title：Cell systems
pub_type： 杂志文章
doi：10.1016/j.cels.2020.11.005
更新日期：2020-12-11 00:00:00
abstract：:Chinese hamster ovary (CHO) cells dominate biotherapeutic protein production and are widely used in mammalian cell line engineering research. To elucidate metabolic bottlenecks in protein production and to guide cell engineering and bioprocess optimization, we reconstructed the metabolic pathways in CHO and associated...
journal_title：Cell systems
pub_type： 杂志文章
doi：10.1016/j.cels.2016.10.020
更新日期：2016-11-23 00:00:00
abstract：:Recent advances in synthetic biology and biological system engineering have allowed the design and construction of engineered live biotherapeutics targeting a range of human clinical applications. In this review, we outline how systems approaches have been used to move from simple constitutive systems, where a single ...
journal_title：Cell systems
pub_type： 杂志文章,评审
doi：10.1016/j.cels.2018.06.008
更新日期：2018-07-25 00:00:00
abstract：:Cell classifiers are genetic logic circuits that transduce endogenous molecular inputs into cell-type-specific responses. Designing classifiers that achieve optimal differential response between specific cell types is a hard computational problem because it involves selection of endogenous inputs and optimization of b...
journal_title：Cell systems
pub_type： 杂志文章
doi：10.1016/j.cels.2017.01.003
更新日期：2017-02-22 00:00:00
abstract：:Two studies show that noise is a key ingredient of new mechanisms for entraining the NF-κB system. ...
journal_title：Cell systems
pub_type： 杂志文章
doi：10.1016/j.cels.2016.12.008
更新日期：2016-12-21 00:00:00
abstract：:Cell-to-cell transcriptional variability in otherwise homogeneous cell populations plays an important role in tissue function and development. Single-cell RNA sequencing can characterize this variability in a transcriptome-wide manner. However, technical variation and the confounding between variability and mean expre...
journal_title：Cell systems
pub_type： 杂志文章
doi：10.1016/j.cels.2018.06.011
更新日期：2018-09-26 00:00:00
abstract：:The field of systems immunology has grown extensively over the last few years, spurred by the generation of large datasets, new analytical tools, and modeling approaches. In this piece and its counterpart in Trends in Immunology [http://www.cell.com/trends/immunology/fulltext/S1471-4906(18)30013-9], eight authors disc...
journal_title：Cell systems
pub_type： 社论,面试
doi：10.1016/j.cels.2018.02.005
更新日期：2018-02-28 00:00:00
abstract：:Adenocarcinoma accounts for more than 40% of lung malignancy, and microscopic pathology evaluation is indispensable for its diagnosis. However, how histopathology findings relate to molecular abnormalities remains largely unknown. Here, we obtained H&E-stained whole-slide histopathology images, pathology reports, RNA ...
journal_title：Cell systems
pub_type： 杂志文章
doi：10.1016/j.cels.2017.10.014
更新日期：2017-12-27 00:00:00
abstract：:Hemizygous deletion of a gene in tumor cells frequently causes reduced expression of its encoded mRNA and protein, as well as reduced protein-but not mRNA-expression of other members in the same protein complex. ...
journal_title：Cell systems
pub_type： 评论,杂志文章
doi：10.1016/j.cels.2017.10.005
更新日期：2017-10-25 00:00:00
abstract：:DNA shape adds specificity to sequence motifs but has not been explored systematically outside this context. We hypothesized that DNA-binding proteins (DBPs) preferentially occupy DNA with specific structures ("shape motifs") regardless of whether or not these correspond to high information content sequence motifs. We...
journal_title：Cell systems
pub_type： 杂志文章
doi：10.1016/j.cels.2018.12.001
更新日期：2019-01-23 00:00:00
abstract：:The human interactome is instrumental in the systems-level study of the cell and the contextualization of disease-associated gene perturbations. However, reference organismal interactomes do not capture the cell-type-specific context in which proteins and modules preferentially act. Here, we introduce SCINET, a comput...
journal_title：Cell systems
pub_type： 杂志文章
doi：10.1016/j.cels.2019.10.007
更新日期：2019-12-18 00:00:00
abstract：:Overlap of RNA and protein networks reveals glia cells as key players for the development of symptomatic Alzheimer's disease in humans. ...
journal_title：Cell systems
pub_type： 评论,杂志文章
doi：10.1016/j.cels.2017.02.006
更新日期：2017-02-22 00:00:00
abstract：:Clinically used RAF inhibitors are ineffective in RAS mutant tumors because they enhance homo- and heterodimerization of RAF kinases, leading to paradoxical activation of ERK signaling. Overcoming enhanced RAF dimerization and the resulting resistance is a challenge for drug design. Combining multiple inhibitors could...
journal_title：Cell systems
pub_type： 杂志文章
doi：10.1016/j.cels.2018.06.002
更新日期：2018-08-22 00:00:00
abstract：:Longitudinal DNA sequencing of cancer patients yields insight into how tumors evolve over time or in response to treatment. However, sequencing data from bulk tumor samples often have considerable ambiguity in clonal composition, complicating the inference of ancestral relationships between clones. We introduce Cancer...
journal_title：Cell systems
pub_type： 杂志文章
doi：10.1016/j.cels.2019.05.010
更新日期：2019-06-26 00:00:00
abstract：:Advances in biological engineering headline this month's Cell Systems call (Cell Systems 1, 307), alongside intriguing applications of modeling from the Elf, Goentoro, and Wolf groups. Check out our recent blogpost: http://crosstalk.cell.com/blog/a-call-for-papers-on-biological-engineering-and-synthetic-biology. ...
journal_title：Cell systems
pub_type： 杂志文章
doi：10.1016/j.cels.2016.08.006
更新日期：2016-08-01 00:00:00
abstract：:Network-based molecular modeling of physiological behaviors has proven invaluable in the study of complex diseases such as cancer, but these approaches remain largely untested in contexts involving interacting tissues such as autoimmunity. Here, using Alopecia Areata (AA) as a model, we have adapted regulatory network...
journal_title：Cell systems
pub_type： 杂志文章
doi：10.1016/j.cels.2015.11.001
更新日期：2015-11-25 00:00:00
